Detailed information    

experimental Experimentally validated

Overview

Name   CJE0566   Type   Regulator
Locus tag   CJE_RS02830 Genome accession   NC_003912
Coordinates   510498..511151 (-) Length   217 a.a.
NCBI ID   WP_011049704.1    Uniprot ID   -
Organism   Campylobacter jejuni RM1221     
Function   repress natural transformation   
Competence regulation

Function

Genetic transfer of the genes to a naturally transformable C. jejuni strain resulted in a decreased efficiency of natural transformation.


Genomic Context

Location: 505498..516151
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CJE_RS02790 (CJE0557) - 506324..506551 (-) 228 WP_002874355.1 helix-turn-helix domain-containing protein -
  CJE_RS02795 (CJE0558) - 506542..506799 (-) 258 WP_002865124.1 hypothetical protein -
  CJE_RS02800 (CJE0559) - 506790..507059 (-) 270 WP_002874357.1 hypothetical protein -
  CJE_RS02805 (CJE0561) - 507621..507893 (-) 273 WP_011049699.1 hypothetical protein -
  CJE_RS02810 (CJE0562) - 508073..508843 (+) 771 WP_041176815.1 SEL1-like repeat protein -
  CJE_RS02815 (CJE0563) - 508862..509245 (-) 384 WP_041176816.1 hypothetical protein -
  CJE_RS02820 (CJE0564) - 509223..509756 (-) 534 WP_011049702.1 hypothetical protein -
  CJE_RS02825 (CJE0565) - 509753..510418 (-) 666 WP_011049703.1 hypothetical protein -
  CJE_RS02830 (CJE0566) CJE0566 510498..511151 (-) 654 WP_011049704.1 DNA/RNA non-specific endonuclease Regulator
  CJE_RS02835 (CJE0567) - 511157..511864 (-) 708 WP_052805202.1 hypothetical protein -
  CJE_RS02840 (CJE0568) - 511857..512645 (-) 789 WP_011049706.1 HipA family kinase -
  CJE_RS02845 (CJE0569) - 512642..513376 (-) 735 WP_011049707.1 S24 family peptidase -
  CJE_RS09610 (CJE0570) - 513488..513712 (+) 225 WP_011049708.1 hypothetical protein -
  CJE_RS02850 (CJE0571) - 513709..514137 (+) 429 WP_011049709.1 hypothetical protein -
  CJE_RS02855 (CJE0572) - 514134..514511 (+) 378 WP_011049710.1 hypothetical protein -
  CJE_RS02860 (CJE0573) - 514508..515509 (+) 1002 WP_011049711.1 hypothetical protein -

Sequence

Protein


Download         Length: 217 a.a.        Molecular weight: 25608.19 Da        Isoelectric Point: 9.7916

>NTDB_id=1224 CJE_RS02830 WP_011049704.1 510498..511151(-) (CJE0566) [Campylobacter jejuni RM1221]
MKKLILLPLLSTLAFADYTQYKPSEEFAKYFTKQSCSQVLDKFYYLNCYDYNLKGTKAVAYKLEVDNLKGEQIKKRPRFE
DDTNIPKKYRTTWSDYKNSGYDRGHTLSNASMRKTTQAQRSTFLMSNITPQNPQINQRVWNKIEKRERQVASKLGSLEVL
NLVNYDNNPQRIRNQIAIPSSYIKILKGENFKECYQVPNHEVEDENIRKYKIDCDKI

Nucleotide


Download         Length: 654 bp        

>NTDB_id=1224 CJE_RS02830 WP_011049704.1 510498..511151(-) (CJE0566) [Campylobacter jejuni RM1221]
ATGAAAAAACTCATACTTTTACCATTGTTATCCACTCTAGCTTTTGCTGACTATACGCAATATAAACCAAGCGAAGAGTT
TGCTAAGTATTTTACTAAGCAAAGTTGTTCGCAAGTTTTAGATAAATTCTATTATCTAAATTGCTATGATTATAATCTAA
AAGGTACTAAAGCAGTGGCTTATAAATTAGAAGTAGATAATCTAAAAGGCGAACAAATCAAAAAGCGCCCGCGCTTTGAA
GATGATACAAATATCCCTAAAAAATATCGTACCACTTGGAGTGATTATAAAAATAGTGGTTATGATAGAGGACACACTCT
TTCTAATGCCTCAATGAGAAAAACAACTCAAGCTCAAAGAAGCACATTCTTAATGAGTAATATTACTCCACAAAATCCAC
AAATCAATCAAAGGGTTTGGAACAAGATTGAAAAAAGAGAAAGACAAGTAGCTTCAAAGCTTGGAAGTTTAGAAGTTTTA
AATTTAGTTAATTATGACAATAATCCACAAAGAATAAGAAATCAAATTGCTATTCCAAGCTCTTATATTAAAATCTTAAA
AGGTGAGAATTTTAAAGAATGTTACCAAGTACCAAATCATGAAGTTGAAGATGAGAATATAAGAAAATATAAGATTGATT
GTGATAAAATATAA

Domains

Predicted by InterProScan.

DNA/RNA non-specific endonuclease (Endonuclease_NS)

(25-216)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  CJE1441 Campylobacter jejuni RM1221

91.163

99.078

0.903

Multiple sequence alignment    



References

[1] Esther J Gaasbeek et al. (2010) Nucleases encoded by the integrated elements CJIE2 and CJIE4 inhibit natural transformation of Campylobacter jejuni. Journal of Bacteriology 192(4):936-41. [PMID: 20023031]