Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AXF35_RS06680 Genome accession   NZ_CP014255
Coordinates   1433382..1434866 (-) Length   494 a.a.
NCBI ID   WP_027221506.1    Uniprot ID   -
Organism   Legionella pneumophila subsp. pascullei strain F-4185     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1428382..1439866
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AXF35_RS06665 (AXF35_10160) cysS 1429341..1430711 (+) 1371 WP_027221503.1 cysteine--tRNA ligase -
  AXF35_RS06670 (AXF35_10155) - 1430883..1432796 (+) 1914 WP_027221504.1 DUF3413 domain-containing protein -
  AXF35_RS06675 (AXF35_10150) - 1432926..1433240 (+) 315 WP_027221505.1 DUF6482 family protein -
  AXF35_RS06680 (AXF35_10145) pilB 1433382..1434866 (-) 1485 WP_027221506.1 GspE family T2SS ATPase variant LspE Machinery gene
  AXF35_RS06685 (AXF35_10140) lspD 1434870..1437245 (-) 2376 WP_027221507.1 GspD family T2SS secretin variant LspD -
  AXF35_RS06690 (AXF35_10135) - 1437417..1438445 (+) 1029 WP_027221508.1 Gfo/Idh/MocA family protein -

Sequence

Protein


Download         Length: 494 a.a.        Molecular weight: 55106.51 Da        Isoelectric Point: 6.0369

>NTDB_id=144872 AXF35_RS06680 WP_027221506.1 1433382..1434866(-) (pilB) [Legionella pneumophila subsp. pascullei strain F-4185]
MENEEKSLKIPYGFAKSNGIVVGEIKENLAVVYHLPNTSLQAFAEIKRLLQCELDLRKVDESTFQQHLANIYQSKSSILD
AAEGMEEDMDLSLLASQLPVSEDLLENQDDAPIIRLLNALFTQAIKQKASDIHIETYEDRVLVRNRIDGVLQEVLEIQRA
IAPLVISRVKVMAKLDIAEKRIPQDGRISLRIGGHNIDVRVSTLPSNHGERVVLRILDKQAAQLDLNLLGMPKHTLKTVR
QMIAEPHGIILVTGPTGSGKTTSLYAMLTELNQVTRNILTIEDPIEYDLPGIGQTQVNTKVQMTFAKGLRAILRQDPDVV
MIGEIRDLETAEIAVQASLTGHLVLSTLHTNTALGALTRLHDMGVESFLLSSSIVGLIAQRLVRKLCSHCKTPHQLREEE
KELMGLKPDADVSQVFEPKGCDYCNHLGYKGRTGIYELIIVDETLRGMIHRNENLQTMENYLRPSTPSIREDGFKRVLCG
DTSLAEILRVTSQN

Nucleotide


Download         Length: 1485 bp        

>NTDB_id=144872 AXF35_RS06680 WP_027221506.1 1433382..1434866(-) (pilB) [Legionella pneumophila subsp. pascullei strain F-4185]
ATGGAAAATGAAGAAAAATCACTTAAAATTCCTTACGGTTTTGCCAAAAGCAATGGAATTGTTGTGGGAGAAATCAAAGA
AAATCTGGCTGTTGTCTATCATTTACCTAACACATCTCTACAAGCTTTCGCTGAGATAAAACGATTATTGCAATGCGAAC
TTGATTTAAGAAAAGTCGATGAGTCCACTTTTCAGCAGCATTTGGCTAATATTTACCAATCCAAATCATCTATACTGGAT
GCTGCTGAAGGCATGGAAGAAGACATGGATTTGTCCTTATTGGCTAGTCAACTACCCGTCAGTGAGGATTTATTGGAAAA
TCAGGATGATGCCCCAATTATCCGTTTACTCAATGCTCTATTCACTCAAGCCATTAAACAAAAAGCATCTGATATACACA
TTGAAACTTATGAAGACAGAGTATTGGTGCGTAACCGAATTGACGGAGTTCTACAAGAAGTCCTTGAAATTCAACGAGCG
ATCGCCCCTTTAGTCATTTCAAGAGTCAAGGTGATGGCCAAATTGGATATAGCAGAAAAGCGAATTCCCCAAGATGGGCG
AATTTCATTGCGTATAGGAGGGCACAATATTGATGTCAGGGTTTCAACTTTACCATCAAACCATGGCGAACGAGTGGTTT
TAAGGATATTGGATAAACAAGCCGCTCAATTGGATTTAAATCTTTTGGGTATGCCTAAACATACACTAAAAACAGTACGA
CAAATGATTGCAGAACCTCATGGCATCATTTTAGTCACAGGCCCTACAGGTTCAGGCAAAACAACCTCTCTCTATGCTAT
GCTGACTGAATTAAATCAGGTGACTCGTAATATTTTAACCATTGAAGATCCCATTGAATATGATTTGCCTGGTATAGGAC
AAACGCAAGTTAATACTAAAGTTCAAATGACTTTCGCCAAAGGATTAAGAGCTATATTGCGTCAAGATCCAGATGTTGTC
ATGATAGGAGAGATCCGTGATTTGGAAACGGCTGAAATTGCGGTTCAAGCCAGCTTAACCGGACATCTGGTACTATCAAC
CCTTCATACCAATACTGCATTGGGTGCGCTAACCCGCTTACATGATATGGGGGTTGAGTCCTTTTTATTGTCATCCAGTA
TAGTTGGCCTCATCGCGCAACGCCTGGTAAGAAAATTATGTTCTCATTGTAAAACACCTCATCAATTAAGAGAGGAAGAA
AAGGAGCTAATGGGTCTTAAGCCGGATGCTGATGTCTCACAAGTCTTTGAACCTAAGGGATGTGACTATTGCAACCACTT
GGGTTATAAAGGCAGAACCGGAATTTATGAATTAATTATCGTAGATGAAACTCTCCGTGGAATGATCCATCGTAATGAAA
ATCTTCAAACCATGGAAAATTATTTGCGGCCATCCACACCAAGTATACGAGAAGATGGTTTTAAAAGAGTATTATGTGGA
GATACGTCACTTGCTGAAATTTTAAGAGTAACCTCTCAAAACTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(119-385)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

43.319

93.927

0.407
  pilB/pilB1 Synechocystis sp. PCC 6803

42.094

90.891

0.383
  pilB Acinetobacter baumannii D1279779

45.433

84.211

0.383
  pilB Vibrio campbellii strain DS40M4

46.305

82.186

0.381
  pilB Acinetobacter baylyi ADP1

48.072

78.745

0.379
  pilB Vibrio parahaemolyticus RIMD 2210633

45.792

81.781

0.374
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.524

79.15

0.36

Multiple sequence alignment