Detailed information    

insolico Bioinformatically predicted

Overview


Name   sinI   Type   Regulator
Locus tag   AAV29_RS12500 Genome accession   NZ_CP011346
Coordinates   2600838..2601011 (+) Length   57 a.a.
NCBI ID   WP_003153105.1    Uniprot ID   A7Z6M7
Organism   Bacillus velezensis strain JJ-D34     
Function   inhibit the expression of sinR (predicted from homology)   
Competence regulation

Genomic Context


Location: 2595838..2606011
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAV29_RS12485 (AAV29_12485) gcvT 2596656..2597756 (-) 1101 WP_014305405.1 glycine cleavage system aminomethyltransferase GcvT -
  AAV29_RS12490 (AAV29_12490) - 2598179..2599849 (+) 1671 WP_046559872.1 DEAD/DEAH box helicase -
  AAV29_RS12495 (AAV29_12495) - 2599867..2600661 (+) 795 WP_014305407.1 YqhG family protein -
  AAV29_RS12500 (AAV29_12500) sinI 2600838..2601011 (+) 174 WP_003153105.1 anti-repressor SinI Regulator
  AAV29_RS12505 (AAV29_12505) sinR 2601045..2601380 (+) 336 WP_003153104.1 transcriptional regulator SinR Regulator
  AAV29_RS12510 (AAV29_12510) tasA 2601428..2602213 (-) 786 WP_003153102.1 biofilm matrix protein TasA -
  AAV29_RS12515 (AAV29_12515) sipW 2602277..2602861 (-) 585 WP_046559873.1 signal peptidase I SipW -
  AAV29_RS12520 (AAV29_12520) tapA 2602833..2603504 (-) 672 WP_046559874.1 amyloid fiber anchoring/assembly protein TapA -
  AAV29_RS12525 (AAV29_12525) - 2603763..2604092 (+) 330 WP_003153097.1 DUF3889 domain-containing protein -
  AAV29_RS12530 (AAV29_12530) - 2604132..2604311 (-) 180 WP_003153093.1 YqzE family protein -
  AAV29_RS12535 (AAV29_12535) comGG 2604368..2604745 (-) 378 WP_046559875.1 competence type IV pilus minor pilin ComGG Machinery gene
  AAV29_RS12540 (AAV29_12540) comGF 2604746..2605141 (-) 396 WP_046559876.1 competence type IV pilus minor pilin ComGF -
  AAV29_RS12545 (AAV29_12545) comGE 2605155..2605469 (-) 315 WP_015388003.1 competence type IV pilus minor pilin ComGE Machinery gene
  AAV29_RS12550 (AAV29_12550) comGD 2605453..2605890 (-) 438 WP_044053464.1 competence type IV pilus minor pilin ComGD Machinery gene

Sequence


Protein


Download         Length: 57 a.a.        Molecular weight: 6695.72 Da        Isoelectric Point: 9.8170

>NTDB_id=144860 AAV29_RS12500 WP_003153105.1 2600838..2601011(+) (sinI) [Bacillus velezensis strain JJ-D34]
MKNAKMEFSQLDKEWVELILEAQKANISLEEIRKYLLSHKKSSQHIQAARSHTVNPF

Nucleotide


Download         Length: 174 bp        

>NTDB_id=144860 AAV29_RS12500 WP_003153105.1 2600838..2601011(+) (sinI) [Bacillus velezensis strain JJ-D34]
ATGAAAAATGCAAAAATGGAATTTTCGCAATTAGATAAGGAATGGGTTGAGCTGATATTAGAAGCCCAAAAAGCAAATAT
CAGCCTAGAAGAAATACGCAAATACCTGCTTTCGCATAAAAAGTCTTCTCAACATATCCAGGCAGCCAGAAGTCATACCG
TAAATCCTTTCTGA

Domains


Predicted by InterproScan.

(11-37)


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A7Z6M7

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sinI Bacillus subtilis subsp. subtilis str. 168

70.175

100

0.702


Multiple sequence alignment