Detailed information NTG0143637

Bioinformatically predicted

Overview

• Name: amiA
• Type: Regulator
• Locus tag: SMQ301_RS06915
• Genome accession: NZ_CP011217
• Coordinates: 1324244..1326211 (-)
• Length: 655 a.a.
• NCBI ID: WP_046206489.1
• Uniprot ID: A0A3G6K5C4
• Organism: Streptococcus thermophilus strain SMQ-301
• Function: internalize XIP (predicted from homology) Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IScluster/Tn	1326567..1335024	1324244..1326211	flank	356

Gene organization within MGE regions

Location: 1324244..1335024

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SMQ301_RS06915 (SMQ301_1430)	amiA	1324244..1326211 (-)	1968	WP_046206489.1	peptide ABC transporter substrate-binding protein	Regulator
SMQ301_RS06920 (SMQ301_1431)	-	1326567..1327744 (+)	1178	Protein_1301	ISL3 family transposase	-
SMQ301_RS06925 (SMQ301_1432)	amiA3	1327789..1329762 (-)	1974	WP_011681419.1	peptide ABC transporter substrate-binding protein	Regulator
SMQ301_RS06935 (SMQ301_1434)	-	1330067..1331214 (+)	1148	WP_095559415.1	IS3 family transposase	-
SMQ301_RS11150	-	1331252..1331436 (-)	185	Protein_1304	IS3 family transposase	-
SMQ301_RS12545	-	1331430..1331840 (-)	411	Protein_1305	ATP-binding cassette domain-containing protein	-
SMQ301_RS06950 (SMQ301_1437)	-	1331872..1332186 (-)	315	Protein_1306	TatD family hydrolase	-
SMQ301_RS06955 (SMQ301_1438)	-	1332532..1333737 (+)	1206	WP_011681420.1	OFA family MFS transporter	-

Sequence

Protein

• Length: 655 a.a.
• Molecular weight: 71731.83 Da
• Isoelectric Point: 5.1056

>NTDB_id=143637 SMQ301_RS06915 WP_046206489.1 1324244..1326211(-) (amiA) [Streptococcus thermophilus strain SMQ-301]
MKLKKILGITGVALLSVGMLAACSSKSSTSGTTYSNIYGSDPETLDYITSIMGGTKDVLTNGVDGLMEADKYGNLVPSVA
EDWSVSKDGLTYTYKIRKGIKWYTSEGEEYANVTAKDFVTGLKHAADAKSGALYLVQDSIAGLSDYLSGANKDFSNVGVK
AIDDYTLQYTLKKQEPYWNSKTTYGVLFPVNEDFLKNKGKDFGKSTDPTSILYNGPFLLKSLTAKSSIELTKNENYWDKK
NVHFDSIKFSYSDGSDLESIERSFSDGALSIARVFPTSSNYASVEKKYKDNIFYTEPGASTSVIGVNIDRQSYKFSAKKT
DAEKTSTKKALLNKDFRQSINFAIDRTAYQSQVNGKDGAALALRNLFVPYDFVSAGDKTFGDLVTEKMSSYGDEWSGVNF
ADGQDGLYNAKKAKTEFAKAKEALQGEGVQFPVHLDLPVDQSSKLSVAQAQSLKQTIEKSLGSENVVIDINQMSSDDMQN
ATSNAANAAAEDWDISNGVGWGPDYQDPSTYLDILKTTSSENTKAFNGYDDPNNAAAAQVGLKDYDALIDSAASETTDLN
ARYDRYAQAQAWLEDSSLVIPLTVSNGAAPIISRLTPFTGASIQVGDKGSSYLKYVKSQEKVVTKKEYEQSREKWLKERK
ASNEKAQKDLEKHIK

Nucleotide

• Length: 1968 bp

>NTDB_id=143637 SMQ301_RS06915 WP_046206489.1 1324244..1326211(-) (amiA) [Streptococcus thermophilus strain SMQ-301]
ATGAAACTTAAAAAAATTCTTGGAATTACGGGTGTAGCCCTTCTAAGTGTTGGTATGTTAGCGGCTTGTTCTTCTAAGTC
AAGTACTAGTGGAACGACTTATAGTAATATTTATGGCTCAGATCCAGAAACCTTGGATTATATTACATCAATTATGGGAG
GGACAAAAGATGTTTTGACTAATGGTGTAGATGGTCTTATGGAAGCGGACAAATATGGTAATCTTGTTCCATCAGTAGCT
GAAGACTGGTCTGTGTCTAAAGATGGTCTTACTTATACCTATAAAATTCGTAAAGGGATCAAATGGTATACTTCTGAAGG
TGAAGAGTACGCTAATGTTACTGCTAAGGACTTTGTAACGGGTCTTAAGCATGCAGCTGATGCCAAATCTGGAGCCCTTT
ATTTGGTACAAGATTCTATTGCTGGATTGTCTGACTACCTTTCTGGTGCCAATAAAGATTTCTCAAATGTTGGCGTTAAA
GCAATCGACGATTACACTCTGCAATACACACTAAAAAAACAGGAACCATACTGGAACTCTAAGACAACCTATGGTGTGCT
CTTCCCAGTTAACGAAGACTTTCTTAAGAACAAAGGGAAAGATTTTGGTAAGTCAACAGACCCAACCTCTATCCTATATA
ACGGTCCTTTCCTTCTTAAATCTTTGACAGCTAAATCATCTATCGAGTTGACTAAGAATGAGAATTATTGGGATAAGAAA
AACGTGCATTTTGACTCTATCAAATTCTCATATTCTGATGGATCGGATCTAGAATCAATAGAACGTAGTTTCTCTGACGG
CGCTCTCAGCATTGCACGTGTCTTCCCTACGAGTTCAAACTATGCATCGGTAGAAAAAAAATATAAAGATAATATTTTTT
ACACAGAACCAGGTGCATCTACATCGGTTATTGGTGTAAACATTGACCGCCAAAGCTATAAATTCTCAGCTAAGAAGACA
GATGCTGAAAAGACATCAACTAAGAAGGCTCTTCTAAACAAAGACTTTCGTCAATCAATCAACTTCGCTATTGATCGTAC
AGCCTATCAATCACAAGTAAACGGTAAAGATGGCGCAGCTCTTGCTCTACGTAACCTTTTTGTTCCTTATGATTTTGTCA
GTGCAGGTGATAAGACCTTTGGTGATCTTGTGACAGAAAAAATGAGCTCTTATGGTGATGAGTGGTCTGGTGTTAACTTT
GCAGATGGTCAAGACGGTCTCTACAATGCTAAAAAAGCTAAGACTGAGTTTGCCAAAGCCAAAGAGGCTCTTCAAGGAGA
AGGGGTACAATTCCCAGTTCACTTAGACCTTCCAGTTGATCAAAGTTCTAAACTTAGTGTTGCTCAAGCTCAATCTTTGA
AACAAACCATTGAAAAATCATTGGGTAGCGAAAATGTTGTTATAGATATTAACCAAATGTCATCTGATGATATGCAAAAT
GCGACTAGTAATGCGGCAAATGCTGCAGCAGAAGACTGGGATATTTCTAACGGTGTGGGTTGGGGTCCAGATTATCAAGA
TCCATCAACTTATCTTGATATCTTAAAAACAACATCAAGTGAAAATACCAAGGCTTTCAATGGTTATGATGATCCAAACA
ATGCAGCAGCAGCCCAAGTAGGTCTTAAAGATTATGATGCCTTGATTGATTCAGCAGCTTCAGAAACAACAGACCTCAAC
GCTCGTTACGACCGTTATGCTCAAGCCCAAGCTTGGTTGGAAGATAGCTCTCTTGTCATTCCATTGACTGTTAGTAACGG
TGCAGCACCGATTATCTCACGTTTGACACCATTTACGGGTGCTAGCATACAGGTGGGTGATAAGGGCTCATCCTACCTCA
AATATGTGAAATCACAAGAAAAAGTAGTTACTAAGAAAGAATATGAACAAAGTCGTGAAAAATGGCTTAAAGAAAGGAAA
GCATCTAACGAAAAAGCTCAAAAAGATCTTGAGAAACACATTAAATAA

Domains

Predicted by InterproScan.

• Bacterial extracellular solute-binding proteins, family 5 Middle (SBP_bac_5) (75-520)

3D structure

• AlphaFold DB: A0A3G6K5C4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiA	Streptococcus salivarius strain HSISS4	89.024	100	0.892
amiA3	Streptococcus thermophilus LMD-9	86.606	100	0.869
amiA3	Streptococcus thermophilus LMG 18311	86.454	100	0.867