Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   bslm_RS12330 Genome accession   NZ_CP011101
Coordinates   2484808..2485425 (-) Length   205 a.a.
NCBI ID   WP_004398514.1    Uniprot ID   A0AAE2SLR2
Organism   Bacillus sp. LM 4-2     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2479808..2490425
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  bslm_RS12305 (BsLM_2513) rpsT 2480110..2480376 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  bslm_RS12310 (BsLM_2514) holA 2480391..2481434 (-) 1044 WP_014480310.1 DNA polymerase III subunit delta -
  bslm_RS21360 (BsLM_2515) - 2481474..2481623 (-) 150 WP_003229985.1 hypothetical protein -
  bslm_RS20840 - 2481664..2481798 (+) 135 WP_003229983.1 YqzM family protein -
  bslm_RS12320 (BsLM_2517) comEC 2481838..2484168 (-) 2331 WP_046381195.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  bslm_RS12325 (BsLM_2518) - 2484172..2484741 (-) 570 WP_046381196.1 ComE operon protein 2 -
  bslm_RS12330 (BsLM_2519) comEA 2484808..2485425 (-) 618 WP_004398514.1 competence protein ComEA Machinery gene
  bslm_RS12335 (BsLM_2520) comER 2485509..2486330 (+) 822 WP_014480313.1 late competence protein ComER -
  bslm_RS12340 (BsLM_2521) - 2486396..2487139 (-) 744 WP_003229973.1 class I SAM-dependent methyltransferase -
  bslm_RS12345 (BsLM_2522) rsfS 2487136..2487492 (-) 357 WP_014480315.1 ribosome silencing factor -
  bslm_RS12350 (BsLM_2523) yqeK 2487510..2488070 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  bslm_RS12355 (BsLM_2524) - 2488060..2488629 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  bslm_RS12360 (BsLM_2525) yhbY 2488641..2488931 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  bslm_RS12365 (BsLM_2526) aroE 2488925..2489767 (-) 843 WP_041351695.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21769.45 Da        Isoelectric Point: 4.7220

>NTDB_id=142204 bslm_RS12330 WP_004398514.1 2484808..2485425(-) (comEA) [Bacillus sp. LM 4-2]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=142204 bslm_RS12330 WP_004398514.1 2484808..2485425(-) (comEA) [Bacillus sp. LM 4-2]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

100

100

1
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39

Multiple sequence alignment