Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYF   Type   Machinery gene
Locus tag   VT08_RS00670 Genome accession   NZ_CP011069
Coordinates   104556..104990 (+) Length   144 a.a.
NCBI ID   WP_002992738.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain STAB09014     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 99556..109990
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VT08_RS00640 (VT08_00640) - 101225..101590 (+) 366 WP_002986560.1 DUF1033 family protein -
  VT08_RS00645 (VT08_00645) comYA 101683..102621 (+) 939 WP_011284420.1 competence type IV pilus ATPase ComGA Machinery gene
  VT08_RS00650 (VT08_00650) comYB 102557..103591 (+) 1035 WP_227868694.1 competence type IV pilus assembly protein ComGB Machinery gene
  VT08_RS00655 (VT08_00655) comYC 103593..103919 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  VT08_RS00660 (VT08_00660) comGD 103894..104322 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  VT08_RS00665 (VT08_00665) comGE 104279..104563 (+) 285 WP_011284422.1 competence type IV pilus minor pilin ComGE -
  VT08_RS00670 (VT08_00670) comYF 104556..104990 (+) 435 WP_002992738.1 competence type IV pilus minor pilin ComGF Machinery gene
  VT08_RS00675 (VT08_00675) comGG 104974..105300 (+) 327 WP_010921801.1 competence type IV pilus minor pilin ComGG -
  VT08_RS00680 (VT08_00680) comYH 105398..106351 (+) 954 WP_010921802.1 class I SAM-dependent methyltransferase Machinery gene
  VT08_RS00685 (VT08_00685) - 106410..107606 (+) 1197 WP_011284424.1 acetate kinase -
  VT08_RS00690 (VT08_00690) - 107793..108101 (+) 309 WP_011284425.1 hypothetical protein -
  VT08_RS00695 (VT08_00695) proC 108184..108954 (-) 771 WP_011284426.1 pyrroline-5-carboxylate reductase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16645.27 Da        Isoelectric Point: 10.2526

>NTDB_id=141963 VT08_RS00670 WP_002992738.1 104556..104990(+) (comYF) [Streptococcus pyogenes strain STAB09014]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIEQSQICITLKWKSGLERTFYYAFQD

Nucleotide


Download         Length: 435 bp        

>NTDB_id=141963 VT08_RS00670 WP_002992738.1 104556..104990(+) (comYF) [Streptococcus pyogenes strain STAB09014]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACCCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAAACCCTCCTTAAACGTAGCCATTATCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGATTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAA
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTTCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGAGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(41-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

51.079

96.528

0.493
  comYF Streptococcus mutans UA159

50.36

96.528

0.486
  comGF Lactococcus lactis subsp. cremoris KW2

46.715

95.139

0.444
  comGF/cglF Streptococcus mitis NCTC 12261

44.776

93.056

0.417
  comGF/cglF Streptococcus pneumoniae Rx1

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae D39

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae R6

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae TIGR4

43.609

92.361

0.403
  comGF/cglF Streptococcus mitis SK321

42.857

92.361

0.396

Multiple sequence alignment