Detailed information    

insolico Bioinformatically predicted

Overview

Name   phrF   Type   Regulator
Locus tag   BIS30_RS08460 Genome accession   NZ_CP011051
Coordinates   1601356..1601475 (+) Length   39 a.a.
NCBI ID   WP_003222023.1    Uniprot ID   A0A9Q4EZF5
Organism   Bacillus spizizenii strain T30     
Function   antagonize RapF (predicted from homology)   
Competence regulation

Genomic Context

Location: 1596356..1606475
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BIS30_RS08435 (BIS30_08435) - 1596527..1597810 (+) 1284 WP_003222013.1 pitrilysin family protein -
  BIS30_RS08440 (BIS30_08440) albG 1597807..1598508 (+) 702 WP_003222015.1 antilisterial bacteriocin subtilosin biosynthesis protein AlbG -
  BIS30_RS08445 (BIS30_08445) - 1598645..1600000 (-) 1356 WP_003222017.1 YncE family protein -
  BIS30_RS08455 (BIS30_08455) rapF 1600227..1601372 (+) 1146 WP_003222022.1 response regulator aspartate phosphatase RapF Regulator
  BIS30_RS08460 (BIS30_08460) phrF 1601356..1601475 (+) 120 WP_003222023.1 phosphatase RapF inhibitor PhrF Regulator
  BIS30_RS08465 (BIS30_08465) speB 1601628..1602500 (-) 873 WP_003222025.1 agmatinase -
  BIS30_RS08470 (BIS30_08470) speE 1602561..1603391 (-) 831 WP_003222027.1 spermidine synthase -
  BIS30_RS08475 (BIS30_08475) - 1603593..1605668 (+) 2076 WP_003222029.1 transglycosylase domain-containing protein -

Sequence

Protein


Download         Length: 39 a.a.        Molecular weight: 4226.12 Da        Isoelectric Point: 10.4997

>NTDB_id=141783 BIS30_RS08460 WP_003222023.1 1601356..1601475(+) (phrF) [Bacillus spizizenii strain T30]
MKLKSKLLLSCLALSTVFIATNIANAPTHQIEVAQRGMI

Nucleotide


Download         Length: 120 bp        

>NTDB_id=141783 BIS30_RS08460 WP_003222023.1 1601356..1601475(+) (phrF) [Bacillus spizizenii strain T30]
ATGAAATTGAAGTCTAAACTATTACTCTCTTGTCTGGCCCTAAGCACTGTTTTCATCGCAACAAATATTGCAAATGCACC
TACCCACCAAATTGAAGTTGCACAGCGAGGAATGATTTAA

Domains

Predicted by InterproScan.

Rap-phr extracellular signalling (PhrC_PhrF)

(3-39)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  phrF Bacillus subtilis subsp. subtilis str. 168

92.593

69.231

0.641
  phrC Bacillus subtilis subsp. subtilis str. 168

48.718

100

0.487

Multiple sequence alignment