Detailed information NTG0141382

Bioinformatically predicted

Overview

• Name: comGA
• Type: Machinery gene
• Locus tag: UP17_RS16290
• Genome accession: NZ_CP011008
• Coordinates: 3508021..3508701 (-)
• Length: 226 a.a.
• NCBI ID: WP_061464028.1
• Uniprot ID: -
• Organism: Peribacillus simplex strain SH-B26
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3503021..3513701

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
UP17_RS16250 (UP17_16235)	-	3503879..3504676 (+)	798	WP_061464021.1	YqhG family protein	-
UP17_RS16255 (UP17_16240)	-	3504726..3504911 (-)	186	WP_061464022.1	YqzE family protein	-
UP17_RS16260 (UP17_16245)	comGG	3505034..3505420 (-)	387	WP_061464023.1	competence type IV pilus minor pilin ComGG	-
UP17_RS16265 (UP17_16250)	comGF	3505413..3505892 (-)	480	WP_061464024.1	competence type IV pilus minor pilin ComGF	-
UP17_RS16270 (UP17_16255)	-	3505840..3506178 (-)	339	WP_061464025.1	hypothetical protein	-
UP17_RS16275 (UP17_16260)	comGD	3506165..3506608 (-)	444	WP_061464026.1	competence type IV pilus minor pilin ComGD	-
UP17_RS16280 (UP17_16265)	comGC	3506616..3506924 (-)	309	WP_284149587.1	competence type IV pilus major pilin ComGC	-
UP17_RS16285 (UP17_16270)	comGB	3507003..3508037 (-)	1035	WP_167555989.1	competence type IV pilus assembly protein ComGB	-
UP17_RS16290 (UP17_16275)	comGA	3508021..3508701 (-)	681	WP_061464028.1	ATPase, T2SS/T4P/T4SS family	Machinery gene
UP17_RS16295 (UP17_16280)	-	3508766..3509683 (+)	918	WP_061464029.1	hypothetical protein	-
UP17_RS16300 (UP17_16285)	-	3510210..3510608 (-)	399	WP_061464030.1	hypothetical protein	-
UP17_RS16305 (UP17_16290)	-	3510899..3511111 (-)	213	WP_061464031.1	hypothetical protein	-
UP17_RS16310	-	3511574..3511954 (-)	381	WP_061464032.1	HNH endonuclease signature motif containing protein	-
UP17_RS16315 (UP17_16300)	-	3512048..3512431 (-)	384	WP_061464033.1	HNH endonuclease	-
UP17_RS16320 (UP17_16305)	-	3512459..3512638 (-)	180	WP_061464034.1	hypothetical protein	-

Sequence

Protein

• Length: 226 a.a.
• Molecular weight: 24843.76 Da
• Isoelectric Point: 7.3881

>NTDB_id=141382 UP17_RS16290 WP_061464028.1 3508021..3508701(-) (comGA) [Peribacillus simplex strain SH-B26]
MNKEKSSLNSLPTGSGKTTTLYSLLHHAHEMINRNIISLEDPIENVSEKVLQVQINEKAGITYSVGLKAVLRHDPDVIMV
GEVRDAETAKIAVRAALTGHLILTTMHTRDAQGAISRLLEFGVSLLEIEQSLIGVTAQRLVELSCVPCKGDCTAIACQMT
ARNKRASVYELLYGKSMANVLRMMGDEKGKGTVSHRQLKDEIGKAVAMGYVDCLEYERLVYDEAKK

Nucleotide

• Length: 681 bp

>NTDB_id=141382 UP17_RS16290 WP_061464028.1 3508021..3508701(-) (comGA) [Peribacillus simplex strain SH-B26]
ATGAATAAAGAAAAATCATCCCTGAATTCCCTTCCAACCGGAAGTGGAAAAACCACCACACTATATTCCCTGCTTCATCA
TGCCCATGAGATGATCAATCGAAATATCATATCACTTGAAGATCCCATTGAAAATGTATCCGAAAAGGTACTGCAAGTCC
AAATCAATGAAAAGGCAGGCATTACTTATTCTGTCGGACTAAAAGCTGTGCTAAGGCATGATCCCGATGTGATCATGGTT
GGGGAGGTCAGGGATGCCGAAACGGCCAAAATTGCAGTTCGTGCTGCACTAACCGGTCATTTGATACTTACAACCATGCA
TACAAGGGATGCACAGGGCGCTATCTCCAGGTTACTTGAATTTGGTGTCAGCTTGCTTGAAATTGAACAGAGTTTGATTG
GCGTGACAGCACAGCGGCTGGTTGAATTAAGCTGTGTACCATGTAAAGGCGACTGTACTGCTATTGCTTGCCAAATGACT
GCCAGGAACAAAAGGGCAAGTGTGTATGAATTGCTTTATGGAAAAAGCATGGCCAATGTCCTTCGGATGATGGGGGATGA
AAAAGGAAAGGGTACGGTAAGCCACCGCCAATTGAAGGATGAAATAGGAAAAGCGGTTGCAATGGGCTATGTGGATTGCC
TGGAATATGAACGGCTGGTATACGATGAAGCAAAAAAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (11-142)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGA	Bacillus subtilis subsp. subtilis str. 168	57.277	94.248	0.54
pilB	Haemophilus influenzae 86-028NP	43.284	88.938	0.385
pilB	Haemophilus influenzae Rd KW20	41.791	88.938	0.372