Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   FORC12_RS03895 Genome accession   NZ_CP010998
Coordinates   782600..783097 (-) Length   165 a.a.
NCBI ID   WP_020978197.1    Uniprot ID   -
Organism   Staphylococcus aureus strain FORC_012 isolate human sputum     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 777600..788097
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC12_RS03870 (FORC12_0722) gcvPB 777762..779234 (-) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  FORC12_RS03875 (FORC12_0723) gcvPA 779227..780573 (-) 1347 WP_000019681.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  FORC12_RS03880 (FORC12_0724) gcvT 780593..781684 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  FORC12_RS03885 (FORC12_0725) - 781843..782367 (-) 525 WP_001015121.1 shikimate kinase -
  FORC12_RS03890 - 782357..782503 (-) 147 WP_001789879.1 hypothetical protein -
  FORC12_RS03895 (FORC12_0726) comGF 782600..783097 (-) 498 WP_020978197.1 competence type IV pilus minor pilin ComGF Machinery gene
  FORC12_RS03900 (FORC12_0727) comGE 783015..783314 (-) 300 WP_000844410.1 hypothetical protein Machinery gene
  FORC12_RS03905 (FORC12_0728) comGD 783301..783747 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  FORC12_RS03910 (FORC12_0729) comGC 783725..784036 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  FORC12_RS03915 (FORC12_0730) comGB 784050..785120 (-) 1071 WP_000776401.1 competence type IV pilus assembly protein ComGB Machinery gene
  FORC12_RS03920 (FORC12_0731) comGA 785092..786066 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  FORC12_RS03925 (FORC12_0732) - 786118..786741 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  FORC12_RS03930 (FORC12_0733) - 786738..787067 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  FORC12_RS03935 (FORC12_0734) - 787067..788053 (-) 987 WP_000161314.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19158.95 Da        Isoelectric Point: 10.1964

>NTDB_id=141162 FORC12_RS03895 WP_020978197.1 782600..783097(-) (comGF) [Staphylococcus aureus strain FORC_012 isolate human sputum]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISIILLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILQKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=141162 FORC12_RS03895 WP_020978197.1 782600..783097(-) (comGF) [Staphylococcus aureus strain FORC_012 isolate human sputum]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAATTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACAGAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(69-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.788

100

0.988
  comGF Staphylococcus aureus MW2

96.364

100

0.964

Multiple sequence alignment