Detailed information NTG0140675

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: TO66_RS26835
• Genome accession: NZ_CP010892
• Coordinates: 5905113..5905985 (+)
• Length: 290 a.a.
• NCBI ID: WP_044465111.1
• Uniprot ID: A0A0C5ERL7
• Organism: Pseudomonas sp. MRSN 12121 strain MRSN12121
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5900113..5910985

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
TO66_RS26820 (TO66_26810)	-	5902504..5902860 (-)	357	WP_044465108.1	BON domain-containing protein	-
TO66_RS26825 (TO66_26815)	-	5903117..5903533 (-)	417	WP_044465109.1	pilin	-
TO66_RS26830 (TO66_26820)	pilC	5903893..5905110 (+)	1218	WP_044465110.1	type II secretion system F family protein	Machinery gene
TO66_RS26835 (TO66_26825)	pilD	5905113..5905985 (+)	873	WP_044465111.1	A24 family peptidase	Machinery gene
TO66_RS26840 (TO66_26830)	coaE	5905982..5906605 (+)	624	WP_044465112.1	dephospho-CoA kinase	-
TO66_RS26845 (TO66_26835)	yacG	5906602..5906805 (+)	204	WP_044465113.1	DNA gyrase inhibitor YacG	-
TO66_RS26850 (TO66_26840)	-	5906810..5907025 (-)	216	WP_044465114.1	hypothetical protein	-
TO66_RS26855 (TO66_26845)	-	5907089..5907778 (-)	690	WP_044465115.1	energy-coupling factor ABC transporter permease	-
TO66_RS26860 (TO66_26850)	-	5907775..5908242 (-)	468	WP_044465116.1	hypothetical protein	-
TO66_RS26865 (TO66_26855)	-	5908645..5910081 (+)	1437	WP_044465117.1	undecaprenyl-phosphate glucose phosphotransferase	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31839.09 Da
• Isoelectric Point: 8.2540

>NTDB_id=140675 TO66_RS26835 WP_044465111.1 5905113..5905985(+) (pilD) [Pseudomonas sp. MRSN 12121 strain MRSN12121]
MSLSEVLASAPLVFVLSTLVLGLIVGSFLNVLVWRLPKMLDQDWRAQAREILGLPAEPAGPTYNLLLPHSQCPHCAHPIR
PWENIPLLSYLLLRGRCSACKGPIGRRYPLVELACGLGSALVAWHFGFGWQAGLMLFLSWGLLAMSLIDADHQLLPDVLV
LPLLWLGLIVNGFALFVSLHDALWGAVAGYLCLWSVYWVFKLLTGKEGIGHGDFKLLALLGAWGGWQILPLTLLLASLTG
ALMGVIMLRRQRAQMSTPIPFGPFLAIAGWIALLWGGQITDLYLQFVGFR

Nucleotide

• Length: 873 bp

>NTDB_id=140675 TO66_RS26835 WP_044465111.1 5905113..5905985(+) (pilD) [Pseudomonas sp. MRSN 12121 strain MRSN12121]
ATGTCATTGAGTGAAGTCCTGGCCAGCGCCCCGCTGGTCTTCGTGTTATCGACGCTGGTCCTGGGCCTGATCGTCGGCAG
CTTCCTCAATGTGCTGGTCTGGCGCCTGCCGAAGATGCTCGACCAGGACTGGCGCGCCCAGGCCCGCGAGATCCTCGGGC
TGCCCGCCGAACCGGCCGGGCCGACCTACAACCTGCTGCTCCCCCACTCCCAATGCCCGCATTGCGCGCACCCGATCCGC
CCCTGGGAAAACATCCCGCTGCTCAGCTACCTGCTGCTGCGCGGCCGCTGCTCCGCCTGCAAGGGCCCGATCGGCCGGCG
CTACCCGCTGGTGGAGCTGGCCTGCGGCCTGGGCTCGGCGTTGGTGGCCTGGCATTTCGGTTTCGGCTGGCAGGCCGGGC
TGATGCTGTTCCTGAGCTGGGGCCTGCTGGCGATGAGCCTGATCGATGCCGATCATCAACTGTTGCCGGACGTGCTGGTG
CTGCCCCTGTTGTGGCTGGGCCTGATCGTCAATGGCTTCGCGCTGTTCGTCAGCCTGCACGACGCGCTGTGGGGCGCCGT
GGCCGGCTACCTGTGCCTGTGGTCGGTGTACTGGGTGTTCAAGCTGCTGACCGGCAAGGAAGGGATCGGCCACGGCGATT
TCAAGCTGCTGGCGCTGCTTGGCGCCTGGGGCGGCTGGCAGATTCTGCCGCTGACACTGCTGCTGGCCTCGCTGACAGGC
GCGCTGATGGGGGTGATCATGCTGCGGCGGCAGCGGGCGCAGATGTCCACGCCCATTCCTTTCGGTCCCTTTCTGGCCAT
CGCCGGCTGGATTGCCTTGCTCTGGGGTGGTCAAATAACCGACCTCTATTTGCAGTTTGTCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

• Type IV leader peptidase family (Peptidase_A24) (137-245)

3D structure

• AlphaFold DB: A0A0C5ERL7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	53.521	97.931	0.524
pilD	Vibrio campbellii strain DS40M4	50	97.241	0.486
pilD	Acinetobacter baumannii D1279779	48.339	93.448	0.452
pilD	Acinetobacter nosocomialis M2	48.339	93.448	0.452
pilD	Neisseria gonorrhoeae MS11	49.012	87.241	0.428