Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   TO66_RS26830 Genome accession   NZ_CP010892
Coordinates   5903893..5905110 (+) Length   405 a.a.
NCBI ID   WP_044465110.1    Uniprot ID   -
Organism   Pseudomonas sp. MRSN 12121 strain MRSN12121     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5898893..5910110
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TO66_RS26815 (TO66_26805) - 5899395..5902316 (+) 2922 WP_044465107.1 DUF748 domain-containing protein -
  TO66_RS26820 (TO66_26810) - 5902504..5902860 (-) 357 WP_044465108.1 BON domain-containing protein -
  TO66_RS26825 (TO66_26815) - 5903117..5903533 (-) 417 WP_044465109.1 pilin -
  TO66_RS26830 (TO66_26820) pilC 5903893..5905110 (+) 1218 WP_044465110.1 type II secretion system F family protein Machinery gene
  TO66_RS26835 (TO66_26825) pilD 5905113..5905985 (+) 873 WP_044465111.1 A24 family peptidase Machinery gene
  TO66_RS26840 (TO66_26830) coaE 5905982..5906605 (+) 624 WP_044465112.1 dephospho-CoA kinase -
  TO66_RS26845 (TO66_26835) yacG 5906602..5906805 (+) 204 WP_044465113.1 DNA gyrase inhibitor YacG -
  TO66_RS26850 (TO66_26840) - 5906810..5907025 (-) 216 WP_044465114.1 hypothetical protein -
  TO66_RS26855 (TO66_26845) - 5907089..5907778 (-) 690 WP_044465115.1 energy-coupling factor ABC transporter permease -
  TO66_RS26860 (TO66_26850) - 5907775..5908242 (-) 468 WP_044465116.1 hypothetical protein -
  TO66_RS26865 (TO66_26855) - 5908645..5910081 (+) 1437 WP_044465117.1 undecaprenyl-phosphate glucose phosphotransferase -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44107.89 Da        Isoelectric Point: 10.6557

>NTDB_id=140674 TO66_RS26830 WP_044465110.1 5903893..5905110(+) (pilC) [Pseudomonas sp. MRSN 12121 strain MRSN12121]
MAVKAAKVRVYLWEGTDRQGRKLRGELSGHTPSLVRAQLRKQGISPGRVRKKAPSLFQRTPPVKARDISLFTRQLATLVK
AGVPLLQAFDIIAEGFDNPNMRKLMEELKQDIAAGSSFTSALRKRPRYFDELYCSLVDAGEQAGALETLLERVATYKEKS
ENLKTRIRKAMTYPLAVIGVALVVTGILLVKVVPQFQAMFSGFGAQLPVFTQLVIALSVFVQQWGWLLAGGLVASILGLR
HANRRSPRFRQWLDALLLKLPVIAPLVHKSAVARYARTLSTTFAAGVPLVEALGSVAGATGNQVFKQAVNRIRQEVATGM
QLHFAMRTSGVFPGMAVQMTAIGEESGALDAMLDKVAIHYEDEVDTLVDTLGSLMEPAIMVVLGTLVGGLVVAMYLPIFQ
LGTAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=140674 TO66_RS26830 WP_044465110.1 5903893..5905110(+) (pilC) [Pseudomonas sp. MRSN 12121 strain MRSN12121]
ATGGCGGTCAAGGCAGCAAAGGTCAGGGTCTACCTGTGGGAAGGCACCGACAGGCAAGGCCGCAAACTGCGCGGCGAATT
GAGCGGCCATACGCCCTCGCTGGTCAGGGCGCAGCTGCGCAAGCAGGGCATCAGCCCGGGCAGGGTCCGCAAGAAAGCCC
CTTCCCTGTTCCAGCGCACGCCCCCCGTAAAGGCACGCGACATCAGCCTGTTCACCCGCCAACTGGCGACCCTGGTCAAG
GCAGGCGTACCGCTGCTGCAAGCCTTCGACATCATTGCCGAGGGCTTCGACAACCCGAACATGCGCAAGCTGATGGAAGA
GCTGAAACAGGACATCGCCGCCGGCAGCAGCTTCACCAGCGCCCTGCGCAAGCGCCCGCGGTATTTCGACGAGCTGTATT
GCAGCCTGGTGGATGCGGGGGAGCAGGCCGGCGCCCTGGAAACCCTGCTGGAGCGGGTAGCGACCTACAAGGAAAAAAGC
GAAAACCTCAAGACCCGGATCCGCAAGGCCATGACCTATCCGCTGGCGGTGATCGGCGTGGCGCTGGTGGTCACCGGCAT
CCTGCTGGTCAAGGTGGTGCCGCAATTCCAGGCGATGTTCTCGGGCTTCGGCGCGCAGTTGCCGGTGTTTACCCAACTGG
TGATCGCCCTTTCGGTATTCGTGCAGCAATGGGGATGGCTGCTGGCGGGCGGCCTGGTCGCCAGCATCCTGGGGCTGCGC
CACGCCAATAGGCGCTCGCCGCGTTTTCGCCAGTGGCTGGATGCCCTCCTGCTGAAGCTGCCGGTGATCGCGCCGCTGGT
GCACAAGTCTGCGGTGGCGCGCTACGCCCGCACCCTGTCCACCACCTTCGCCGCTGGAGTACCGCTGGTGGAGGCCCTGG
GCTCGGTGGCCGGTGCCACGGGCAACCAGGTGTTCAAGCAGGCGGTGAACCGGATCAGGCAGGAAGTGGCGACCGGCATG
CAACTGCACTTCGCCATGCGCACCAGCGGCGTGTTTCCCGGCATGGCCGTCCAGATGACCGCCATCGGCGAAGAGTCGGG
CGCCCTGGACGCCATGCTGGACAAGGTCGCGATCCACTACGAGGACGAGGTCGACACCCTGGTCGATACCCTCGGCAGTC
TCATGGAGCCCGCGATCATGGTAGTTTTGGGCACCCTCGTCGGCGGCCTGGTGGTCGCCATGTACTTACCCATCTTCCAA
CTCGGCACAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

64.938

100

0.649
  pilC Acinetobacter baylyi ADP1

56.863

100

0.573
  pilC Acinetobacter baumannii D1279779

55.854

100

0.565
  pilC Legionella pneumophila strain ERS1305867

51.768

97.778

0.506
  pilC Vibrio cholerae strain A1552

42.145

99.012

0.417
  pilG Neisseria gonorrhoeae MS11

42.04

99.259

0.417
  pilG Neisseria meningitidis 44/76-A

41.294

99.259

0.41
  pilC Vibrio campbellii strain DS40M4

40.05

99.259

0.398
  pilC Thermus thermophilus HB27

37.065

99.259

0.368

Multiple sequence alignment