Detailed information    

insolico Bioinformatically predicted

Overview

Name   recG   Type   Machinery gene
Locus tag   ASZ83_RS01185 Genome accession   NZ_CP013315
Coordinates   226298..228376 (-) Length   692 a.a.
NCBI ID   WP_000080593.1    Uniprot ID   Q9KNM1
Organism   Vibrio cholerae strain M2140     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 221298..233376
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ASZ83_RS01165 (ASZ83_00222) greB 221792..222277 (+) 486 WP_000850496.1 transcription elongation factor GreB -
  ASZ83_RS01170 (ASZ83_00223) ompR 222415..223137 (+) 723 WP_000230023.1 two-component system response regulator OmpR -
  ASZ83_RS01175 (ASZ83_00224) envZ 223206..224522 (+) 1317 WP_001222687.1 two-component system sensor histidine kinase EnvZ -
  ASZ83_RS01180 (ASZ83_00225) - 224547..225953 (-) 1407 WP_000193487.1 uracil-xanthine permease family protein -
  ASZ83_RS01185 (ASZ83_00226) recG 226298..228376 (-) 2079 WP_000080593.1 ATP-dependent DNA helicase RecG Machinery gene
  ASZ83_RS01190 (ASZ83_00227) spoT 228495..230612 (-) 2118 WP_000280450.1 bifunctional GTP diphosphokinase/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase -
  ASZ83_RS01195 (ASZ83_00228) rpoZ 230644..230916 (-) 273 WP_000135052.1 DNA-directed RNA polymerase subunit omega -
  ASZ83_RS01200 (ASZ83_00229) gmk 231024..231647 (-) 624 WP_000515790.1 guanylate kinase -
  ASZ83_RS01205 (ASZ83_00230) - 232015..232686 (+) 672 WP_001015358.1 7-cyano-7-deazaguanine/7-aminomethyl-7- deazaguanine transporter -

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 76933.18 Da        Isoelectric Point: 7.7534

>NTDB_id=140530 ASZ83_RS01185 WP_000080593.1 226298..228376(-) (recG) [Vibrio cholerae strain M2140]
MSQLLSAVPLCELSGVGAKVAEKLEKVGLHTVQDLLFHLPLRYEDRTRVYPIVQLHHGLWAAVQGKVMAVDTLFGKRKML
TVKISDGNGTLTLRFFNFTAAMKNNFAEGKFVHAYGEIKRGNQGLEIIHPDYKFFTPAQTPDVEPNLTPVYPTTEGLRQL
TLRNLTDQALVLLEKSAVQELLPSGLYDQQMTLAQALKIIHRPSADIDLRLFEQGRHPAQIRLIMEELLAQNLSMLAIRS
QGQQDVALPLAPVHQLKQQLLAQLPFSPTKAQQRVVAEIEADLAKPHPMMRLVQGDVGSGKTLVAALAAVRAIEHGYQVA
LMAPTELLAEQHALNFAQWLEPMGIQVGWLAGKLKGKARETELARIASGEVKMVVGTHALFQEQVSFDHLALVIIDEQHR
FGVHQRLELREKGAKQGAYPHQLIMTATPIPRTLAMTAYADLETSVIDELPPGRTPIQTVAIPDTKRDEIVERIRHACLN
EGKQAYWVCTLIDESEVLEAQAAAETAEELQRKLPEVKIGLVHGRMKPAEKQAVMQAFKNNELHLLVATTVIEVGVDVPN
ASLMIIENPERLGLAQLHQLRGRVGRGTVASHCVLLFHAPLSKTAQKRLGVLRESNDGFVIAQRDLEIRGPGELLGTKQT
GLADFKIADLVRDQQLVPQVQRIARHIHERYPQNAQAIIDRWLGERDIYAKA

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=140530 ASZ83_RS01185 WP_000080593.1 226298..228376(-) (recG) [Vibrio cholerae strain M2140]
ATGTCACAACTCCTTTCAGCCGTTCCTCTTTGTGAACTCTCCGGTGTCGGGGCCAAAGTCGCCGAAAAACTGGAGAAAGT
CGGTTTGCACACGGTGCAGGATCTGCTGTTTCATCTTCCACTGCGTTATGAAGATCGCACTCGCGTTTATCCGATAGTGC
AACTGCACCACGGTTTATGGGCCGCGGTACAAGGCAAAGTGATGGCAGTGGATACCCTGTTTGGCAAACGCAAAATGCTC
ACCGTGAAAATCAGTGATGGCAACGGTACACTGACACTGCGTTTTTTTAACTTCACCGCGGCGATGAAAAACAACTTTGC
GGAAGGCAAATTTGTGCATGCCTATGGGGAAATCAAACGCGGTAATCAAGGCTTAGAGATCATCCATCCTGACTACAAAT
TCTTTACCCCTGCACAAACGCCCGATGTTGAGCCAAACCTGACTCCGGTTTACCCAACCACTGAAGGACTGCGCCAACTC
ACCCTACGTAATTTGACTGACCAAGCGTTGGTGCTACTGGAAAAATCTGCGGTGCAGGAGTTACTGCCATCCGGACTTTA
CGATCAGCAGATGACCTTAGCCCAAGCGCTGAAGATCATTCATCGCCCATCGGCGGATATCGATTTGCGCCTGTTTGAAC
AAGGGCGACATCCGGCGCAAATTCGGCTGATCATGGAAGAGCTGCTGGCGCAAAATCTCTCTATGCTCGCGATTCGCAGT
CAAGGCCAGCAAGATGTGGCCTTGCCACTGGCACCCGTCCACCAACTCAAACAACAACTCTTAGCACAGCTTCCCTTTTC
ACCGACTAAGGCGCAGCAGCGCGTGGTTGCCGAAATCGAAGCCGATTTAGCCAAGCCGCACCCAATGATGCGCTTAGTGC
AAGGCGATGTCGGCTCAGGCAAAACCTTAGTCGCAGCGCTTGCGGCAGTGCGGGCGATTGAGCATGGCTATCAAGTAGCC
TTGATGGCTCCTACCGAGCTACTGGCCGAGCAGCACGCGCTCAATTTTGCGCAGTGGCTAGAGCCGATGGGCATTCAAGT
CGGCTGGCTCGCGGGTAAGCTCAAAGGCAAAGCGCGGGAAACGGAGCTGGCACGCATCGCCAGTGGCGAAGTGAAAATGG
TGGTTGGCACCCATGCTCTGTTTCAAGAGCAAGTGTCGTTTGACCATCTTGCCTTGGTCATTATTGATGAGCAGCATCGC
TTCGGGGTACATCAGCGTTTAGAGCTGCGCGAGAAAGGCGCAAAACAAGGCGCGTACCCGCATCAGCTCATTATGACTGC
TACTCCGATCCCGCGTACTTTGGCGATGACGGCTTATGCCGATTTGGAAACCTCAGTGATTGATGAGTTACCCCCCGGGC
GTACTCCGATTCAAACCGTAGCGATTCCGGATACCAAACGCGATGAGATTGTCGAGCGCATTCGCCACGCCTGCCTCAAC
GAAGGCAAGCAAGCGTATTGGGTGTGTACTTTGATTGATGAATCCGAAGTGCTGGAAGCGCAAGCGGCCGCAGAAACGGC
CGAGGAACTGCAACGTAAACTGCCCGAAGTCAAAATCGGCTTAGTGCATGGCCGGATGAAACCCGCCGAAAAACAAGCGG
TAATGCAGGCGTTTAAAAACAATGAGCTGCATCTGCTCGTCGCCACCACAGTGATTGAAGTGGGCGTGGATGTGCCTAAT
GCCAGCTTGATGATCATTGAAAACCCCGAACGTTTGGGGCTAGCTCAACTTCACCAGCTACGCGGCCGTGTTGGGCGTGG
TACCGTAGCCAGCCATTGTGTGCTGCTGTTTCATGCACCACTCTCCAAAACCGCGCAAAAGCGTTTGGGCGTTTTGCGTG
AAAGCAACGATGGATTTGTCATCGCGCAGCGCGACTTGGAAATTCGCGGCCCCGGAGAGCTATTAGGCACCAAACAAACC
GGCTTAGCCGATTTCAAAATTGCTGATCTGGTACGCGATCAGCAACTGGTTCCGCAAGTGCAACGCATCGCGCGACATAT
CCATGAACGCTACCCACAGAATGCACAAGCCATCATCGACCGCTGGTTAGGCGAGCGCGATATTTACGCCAAAGCTTAG

Domains

Predicted by InterProScan.

RecG wedge domain (RecG_wedge)

(15-170)

Helicase conserved C-terminal domain (Helicase_C)

(479-587)

ATP-dependent DNA helicase RecG, domain 3, C-terminal (RecG_dom3_C)

(616-674)

DEAD/DEAH box helicase (DEAD)

(271-432)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9KNM1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recG Neisseria meningitidis strain C311

50.893

97.11

0.494
  recG/mmsA Streptococcus pneumoniae R6

39.227

97.254

0.381
  recG/mmsA Streptococcus pneumoniae R36A

39.227

97.254

0.381
  recG Bacillus subtilis subsp. subtilis str. 168

39.478

94.075

0.371