Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ASZ84_RS10985 Genome accession   NZ_CP013307
Coordinates   2383513..2384103 (-) Length   196 a.a.
NCBI ID   WP_000157690.1    Uniprot ID   A0A366AJW1
Organism   Vibrio cholerae strain E506     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2378513..2389103
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ASZ84_RS10965 (ASZ84_02167) aroB 2379440..2380525 (-) 1086 WP_000439805.1 3-dehydroquinate synthase -
  ASZ84_RS10970 (ASZ84_02168) aroK 2380553..2381077 (-) 525 WP_000818616.1 shikimate kinase AroK -
  ASZ84_RS10975 (ASZ84_02169) pilQ 2381264..2382979 (-) 1716 WP_000788426.1 type IV pilus secretin PilQ Machinery gene
  ASZ84_RS10980 (ASZ84_02170) pilP 2383005..2383523 (-) 519 WP_000792032.1 pilus assembly protein PilP Machinery gene
  ASZ84_RS10985 (ASZ84_02171) pilO 2383513..2384103 (-) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  ASZ84_RS10990 (ASZ84_02172) pilN 2384096..2384680 (-) 585 WP_000902745.1 PilN domain-containing protein Machinery gene
  ASZ84_RS10995 (ASZ84_02173) pilM 2384670..2385149 (-) 480 Protein_2069 type IV pilus assembly protein PilM -
  ASZ84_RS11000 (ASZ84_02174) - 2385418..2386155 (-) 738 WP_000005494.1 LexA family transcriptional regulator -
  ASZ84_RS11005 (ASZ84_02175) - 2386308..2386532 (+) 225 WP_000373567.1 helix-turn-helix domain-containing protein -
  ASZ84_RS11010 (ASZ84_02176) - 2386544..2388547 (+) 2004 WP_001895336.1 transposase domain-containing protein -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22877.27 Da        Isoelectric Point: 4.4169

>NTDB_id=140249 ASZ84_RS10985 WP_000157690.1 2383513..2384103(-) (pilO) [Vibrio cholerae strain E506]
MASLQELELDEISEWPLLPQLVVILLIMLLIQGAGYWFYLMPKQDEIALLKQEEETVKATLRIKANKVAVLPQIQAQLDE
LKERYDFLLRQLPVQKELASMLASVNQLGLDSSLTFTRIDWGERESQEFLYRLPLNIELTGSYHDIGDFSQAIAKLPRII
NFDDVDWQRVSQESSTLHFRVRAYTYQFKQEVDDEK

Nucleotide


Download         Length: 591 bp        

>NTDB_id=140249 ASZ84_RS10985 WP_000157690.1 2383513..2384103(-) (pilO) [Vibrio cholerae strain E506]
ATGGCTAGTCTACAAGAGCTAGAACTGGATGAAATTAGTGAATGGCCGCTGCTGCCTCAGTTGGTGGTGATTCTGCTTAT
CATGCTACTGATCCAAGGTGCTGGTTATTGGTTCTATCTCATGCCCAAACAAGACGAGATTGCGCTGTTAAAGCAGGAAG
AGGAAACCGTCAAGGCGACACTGCGCATCAAAGCCAATAAAGTGGCGGTATTGCCGCAGATCCAAGCTCAGTTGGACGAG
CTCAAAGAGCGTTACGATTTTTTACTGCGCCAGCTACCGGTGCAAAAAGAGTTAGCCAGCATGCTGGCGTCAGTCAACCA
GCTTGGGCTCGATAGTTCGCTTACCTTTACGCGGATTGATTGGGGGGAGCGTGAAAGCCAAGAGTTTCTTTACCGCTTAC
CGCTCAATATCGAACTGACGGGGAGCTATCACGATATTGGCGATTTTTCGCAAGCGATTGCCAAGTTACCGCGCATCATC
AACTTCGACGATGTGGACTGGCAGCGTGTCAGCCAAGAGAGTAGCACGCTGCACTTTCGAGTCAGAGCCTACACCTACCA
ATTTAAGCAGGAGGTGGATGATGAAAAATAA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(50-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A366AJW1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

100

100

1
  pilO Vibrio campbellii strain DS40M4

71.958

96.429

0.694