Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   TK49_RS05095 Genome accession   NZ_CP010799
Coordinates   1052808..1054307 (-) Length   499 a.a.
NCBI ID   WP_045785734.1    Uniprot ID   -
Organism   Ralstonia mannitolilytica strain SN82F48     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1047808..1059307
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  TK49_RS05065 (TK49_05070) - 1048319..1048972 (-) 654 WP_045785729.1 LON peptidase substrate-binding domain-containing protein -
  TK49_RS05070 (TK49_05075) rapZ 1049065..1049955 (-) 891 WP_045785730.1 RNase adapter RapZ -
  TK49_RS05075 (TK49_05080) - 1050050..1050373 (-) 324 WP_045785731.1 PsiF family protein -
  TK49_RS05080 (TK49_05085) hprK 1050560..1051534 (-) 975 WP_045785732.1 HPr(Ser) kinase/phosphatase -
  TK49_RS05085 (TK49_05090) ptsN 1051605..1052060 (-) 456 WP_004633390.1 PTS IIA-like nitrogen regulatory protein PtsN -
  TK49_RS05090 (TK49_05095) hpf 1052403..1052756 (-) 354 WP_045785733.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  TK49_RS05095 (TK49_05100) rpoN/rpoN1 1052808..1054307 (-) 1500 WP_045785734.1 RNA polymerase factor sigma-54 Machinery gene
  TK49_RS05100 (TK49_05105) lptB 1054415..1055203 (-) 789 WP_045785735.1 LPS export ABC transporter ATP-binding protein -
  TK49_RS05105 (TK49_05110) lptA 1055232..1055798 (-) 567 WP_045785736.1 lipopolysaccharide transport periplasmic protein LptA -
  TK49_RS05110 (TK49_05115) lptC 1055857..1056489 (-) 633 WP_045785737.1 LPS export ABC transporter periplasmic protein LptC -
  TK49_RS05115 (TK49_05120) - 1056494..1057078 (-) 585 WP_045785738.1 KdsC family phosphatase -
  TK49_RS05120 (TK49_05125) - 1057095..1058078 (-) 984 WP_045785739.1 KpsF/GutQ family sugar-phosphate isomerase -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 55341.80 Da        Isoelectric Point: 4.9093

>NTDB_id=139874 TK49_RS05095 WP_045785734.1 1052808..1054307(-) (rpoN/rpoN1) [Ralstonia mannitolilytica strain SN82F48]
MKQSLQLRLSQHLALTPQLQQSIRLLQLSTIELQQEVEQALTENPLLERENEWLDTSARIGADGSVNALQSSSATPLPTE
SPPPAPNGTDSTDAPETSYESSADFDSDYGSDRSDWSLDDFARKPQSDEDDRAPMQLREAEPTLREFLMEQLAPLKLSMR
DKGLVIFLIESLDDEGYLSGTLDEILADLPSELEVELDELQAALRMLQSFDPPGIGARSAAECLSLQLHRLESPAKTLAL
TIVNHHLELLAARDYTRLKKALSVDEPALKAAHELIRSLAPFPGHAFGRAEADFVVPDVVVRKTSAGWMAQLNPEVMPRL
RINDMYAQILRSSRGEAGAANLQQKLQEARWLIKNIQQRFDTILRVSQAIVERQKSFFTHGEIAMRPLVLREIADTLGLH
ESTISRVTTNKYMATPMGTFELKYFFGSHVSTETGGAASSTAIRALIKQLVGAEDPKNPLSDSRIAELLGEQGFVVARRT
VAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=139874 TK49_RS05095 WP_045785734.1 1052808..1054307(-) (rpoN/rpoN1) [Ralstonia mannitolilytica strain SN82F48]
ATGAAACAGTCGCTCCAGCTTCGTCTCTCTCAGCATCTGGCGCTCACGCCGCAGTTGCAGCAGTCGATCCGGCTGCTGCA
GTTGTCGACCATCGAACTCCAGCAGGAAGTCGAACAGGCTCTGACCGAAAACCCGCTGCTGGAGCGGGAAAACGAGTGGC
TCGACACGTCCGCGCGCATCGGCGCAGATGGCTCCGTCAACGCGCTGCAAAGCAGCAGCGCCACACCGCTGCCCACCGAA
TCGCCACCGCCCGCGCCCAACGGCACGGACAGCACCGACGCTCCCGAAACCAGCTACGAGAGCAGCGCCGACTTCGACAG
CGATTACGGATCCGACCGCTCCGACTGGAGCCTCGACGACTTCGCCCGCAAGCCGCAATCGGACGAAGACGACCGCGCGC
CCATGCAGCTGCGTGAAGCCGAGCCGACGCTGCGCGAATTCCTCATGGAGCAGCTCGCGCCGCTCAAGCTGTCGATGCGC
GACAAGGGCCTCGTCATCTTCCTGATCGAATCGCTCGATGACGAAGGCTATCTCTCGGGCACACTCGACGAAATCCTCGC
CGATCTGCCGTCGGAACTCGAGGTCGAACTCGACGAACTGCAAGCCGCGCTGCGCATGCTGCAGAGCTTCGACCCGCCCG
GTATCGGCGCGCGCTCGGCCGCCGAATGCCTGTCGCTGCAGTTGCACCGGCTCGAATCACCGGCGAAGACGCTCGCGCTC
ACGATCGTCAATCACCATCTCGAACTGCTCGCCGCACGCGATTACACACGGCTGAAGAAGGCACTGTCCGTCGACGAGCC
GGCGCTCAAGGCGGCACACGAGCTCATTCGTTCATTGGCGCCGTTTCCAGGGCATGCGTTCGGCCGCGCAGAGGCCGACT
TTGTCGTGCCCGACGTCGTCGTGCGCAAGACCAGTGCGGGCTGGATGGCGCAGTTGAATCCCGAAGTGATGCCGCGTCTG
CGCATCAACGACATGTACGCGCAGATCCTGCGCAGCAGCCGCGGTGAAGCCGGCGCCGCCAACCTGCAGCAGAAGCTGCA
GGAAGCACGTTGGCTGATCAAGAACATCCAGCAGCGTTTCGATACGATCCTGCGTGTCTCGCAAGCCATTGTCGAGCGTC
AAAAGAGCTTCTTCACGCACGGTGAAATCGCCATGCGGCCCTTGGTTTTGCGGGAAATAGCCGATACACTGGGTCTACAC
GAGTCCACGATCTCCCGTGTGACGACCAACAAGTACATGGCGACCCCCATGGGCACCTTCGAGCTGAAGTACTTCTTCGG
CAGCCATGTATCGACGGAAACCGGCGGGGCTGCGTCATCCACTGCGATACGCGCGCTCATCAAGCAACTTGTAGGAGCCG
AAGACCCGAAGAATCCTCTGTCCGATAGCAGAATTGCCGAGCTGTTAGGCGAACAAGGATTCGTGGTGGCCCGTCGGACG
GTGGCCAAGTACCGCGAAGCCCTGAAGATCCCAGCAGTGAATCTGCGCAAGTCCTTATAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(136-326)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(341-497)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

94.188

100

0.942

Multiple sequence alignment