Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilD   Type   Machinery gene
Locus tag   PG1C_RS00895 Genome accession   NZ_CP010554
Coordinates   161758..162615 (-) Length   285 a.a.
NCBI ID   WP_202635577.1    Uniprot ID   A0A0C5J6W4
Organism   Rugosibacter aromaticivorans strain Ca6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 156758..167615
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PG1C_RS00855 (PG1C_00830) - 156895..157392 (+) 498 WP_202635569.1 DoxX family protein -
  PG1C_RS14940 - 157389..157604 (+) 216 WP_202635570.1 cysteine-rich CWC family protein -
  PG1C_RS00865 (PG1C_00835) nudB 157579..158019 (-) 441 WP_202635571.1 dihydroneopterin triphosphate diphosphatase -
  PG1C_RS00870 - 158043..158507 (-) 465 WP_202635572.1 barstar family protein -
  PG1C_RS00875 (PG1C_00845) - 158504..158848 (-) 345 WP_202635573.1 ribonuclease domain-containing protein -
  PG1C_RS00880 (PG1C_00850) aspS 158855..160660 (-) 1806 WP_202635574.1 aspartate--tRNA ligase -
  PG1C_RS00885 (PG1C_00855) - 160709..161329 (-) 621 WP_202635575.1 DUF502 domain-containing protein -
  PG1C_RS00890 (PG1C_00860) - 161359..161673 (-) 315 WP_202635576.1 FmdB family zinc ribbon protein -
  PG1C_RS00895 (PG1C_00865) pilD 161758..162615 (-) 858 WP_202635577.1 prepilin peptidase Machinery gene
  PG1C_RS00900 (PG1C_00870) pilC 162625..163857 (-) 1233 WP_202635578.1 type II secretion system F family protein Machinery gene
  PG1C_RS00905 (PG1C_00875) - 163915..165186 (-) 1272 WP_202635579.1 HlyC/CorC family transporter -
  PG1C_RS00910 (PG1C_00880) - 165210..166058 (-) 849 WP_202635580.1 inner membrane protein YpjD -
  PG1C_RS00915 (PG1C_00885) ffh 166099..167454 (+) 1356 WP_202635581.1 signal recognition particle protein -

Sequence


Protein


Download         Length: 285 a.a.        Molecular weight: 31038.00 Da        Isoelectric Point: 8.3327

>NTDB_id=139171 PG1C_RS00895 WP_202635577.1 161758..162615(-) (pilD) [Rugosibacter aromaticivorans strain Ca6]
MSALWFTDPSVVVLIASVLGLMVGSFLNVVIHRLPIMMERDWAVQCADLRGDTPPVFEPLSLSRPRSRCPKCSHAITATE
NIPLFSWFFLRGRCSACKTPISIRYPLIELATAALFAFAAMHFGLTAAGWGALIFIAALISLTGIDYDTRLLPDDITLPL
LWIGLLFNLFGTYTDLKSAVIGAIAGYLSLWSVYWLFKLVTGKEGMGYGDFKLLAALGAWLGWQMLPLTILLSSLVGAVV
GILLMVMSKHGRQEPIPFGPYLAAAGIVALIWGKPLTQAYLGTTL

Nucleotide


Download         Length: 858 bp        

>NTDB_id=139171 PG1C_RS00895 WP_202635577.1 161758..162615(-) (pilD) [Rugosibacter aromaticivorans strain Ca6]
ATGAGCGCCTTGTGGTTTACGGATCCGTCGGTGGTTGTTTTGATCGCCAGCGTGCTGGGCTTGATGGTTGGCAGCTTTCT
TAATGTCGTGATTCACCGTTTGCCTATCATGATGGAGCGCGATTGGGCCGTGCAATGTGCCGATTTGCGCGGTGACACGC
CACCCGTTTTTGAGCCACTCAGTCTGTCTCGTCCGCGCTCGCGTTGCCCCAAATGTAGCCATGCCATTACGGCGACTGAG
AATATCCCGCTCTTTAGCTGGTTTTTTCTGCGTGGCCGTTGCAGCGCTTGCAAAACACCGATCTCTATTCGCTACCCCTT
GATAGAACTAGCGACAGCGGCGTTGTTTGCCTTCGCTGCCATGCACTTTGGTTTAACTGCTGCGGGTTGGGGCGCGCTAA
TTTTTATCGCGGCGCTGATTTCACTCACAGGCATCGACTACGATACGCGACTGCTACCCGATGACATTACGCTACCGCTG
CTATGGATTGGTTTGCTATTTAATCTGTTTGGCACATATACCGATCTTAAAAGTGCAGTGATTGGGGCTATAGCAGGCTA
TCTGTCCCTTTGGAGCGTGTATTGGCTATTCAAGCTTGTCACCGGCAAAGAGGGCATGGGTTACGGCGACTTTAAATTAT
TGGCGGCCCTCGGTGCCTGGCTGGGGTGGCAAATGCTTCCCTTGACTATTCTGTTATCCTCCCTGGTGGGCGCCGTGGTC
GGCATCCTGCTCATGGTGATGTCAAAACACGGCCGCCAGGAGCCCATTCCTTTCGGGCCTTATCTTGCCGCAGCGGGGAT
CGTTGCCTTGATCTGGGGCAAACCGCTCACTCAGGCTTATCTCGGCACCACGCTGTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A0C5J6W4

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

55.282

99.649

0.551

  pilD Vibrio campbellii strain DS40M4

53.214

98.246

0.523

  pilD Acinetobacter nosocomialis M2

50

98.947

0.495

  pilD Acinetobacter baumannii D1279779

49.291

98.947

0.488

  pilD Neisseria gonorrhoeae MS11

52.918

90.175

0.477


Multiple sequence alignment