Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   RU53_RS08435 Genome accession   NZ_CP010526
Coordinates   1692358..1692855 (-) Length   165 a.a.
NCBI ID   WP_001829055.1    Uniprot ID   -
Organism   Staphylococcus aureus subsp. aureus ST772-MRSA-V strain DAR4145     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1687358..1697855
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RU53_RS08410 (RU53_1634) gcvPB 1687520..1688992 (-) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  RU53_RS08415 (RU53_1635) gcvPA 1688985..1690331 (-) 1347 WP_000019683.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  RU53_RS08420 (RU53_1636) gcvT 1690351..1691442 (-) 1092 WP_000093346.1 glycine cleavage system aminomethyltransferase GcvT -
  RU53_RS08425 (RU53_1638) - 1691601..1692125 (-) 525 WP_001015121.1 shikimate kinase -
  RU53_RS15325 - 1692115..1692261 (-) 147 WP_001789879.1 hypothetical protein -
  RU53_RS08435 (RU53_1639) comGF 1692358..1692855 (-) 498 WP_001829055.1 competence type IV pilus minor pilin ComGF Machinery gene
  RU53_RS08440 (RU53_1640) comGE 1692773..1693072 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  RU53_RS08445 (RU53_1641) comGD 1693059..1693505 (-) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  RU53_RS08450 (RU53_1642) comGC 1693483..1693794 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  RU53_RS08455 (RU53_1643) comGB 1693808..1694878 (-) 1071 WP_000776406.1 competence type IV pilus assembly protein ComGB Machinery gene
  RU53_RS08460 (RU53_1644) comGA 1694850..1695824 (-) 975 WP_000697227.1 competence type IV pilus ATPase ComGA Machinery gene
  RU53_RS08465 (RU53_1645) - 1695876..1696499 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  RU53_RS08470 (RU53_1646) - 1696496..1696825 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  RU53_RS08475 (RU53_1647) - 1696825..1697811 (-) 987 WP_000161314.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19183.92 Da        Isoelectric Point: 10.2192

>NTDB_id=139075 RU53_RS08435 WP_001829055.1 1692358..1692855(-) (comGF) [Staphylococcus aureus subsp. aureus ST772-MRSA-V strain DAR4145]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=139075 RU53_RS08435 WP_001829055.1 1692358..1692855(-) (comGF) [Staphylococcus aureus subsp. aureus ST772-MRSA-V strain DAR4145]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(68-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.182

100

0.982
  comGF Staphylococcus aureus MW2

98.182

100

0.982

Multiple sequence alignment