Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   AO356_RS09420 Genome accession   NZ_CP012831
Coordinates   2129946..2130569 (-) Length   207 a.a.
NCBI ID   WP_060739559.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain FW300-N2C3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2124946..2135569
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AO356_RS09400 (AO356_09385) aroB 2125514..2126614 (-) 1101 WP_060739555.1 3-dehydroquinate synthase -
  AO356_RS09405 (AO356_09390) aroK 2126794..2127312 (-) 519 WP_060739556.1 shikimate kinase AroK -
  AO356_RS09410 (AO356_09395) pilQ 2127317..2129404 (-) 2088 WP_060739557.1 type IV pilus secretin PilQ Machinery gene
  AO356_RS09415 (AO356_09400) - 2129401..2129949 (-) 549 WP_060739558.1 pilus assembly protein PilP -
  AO356_RS09420 (AO356_09405) pilO 2129946..2130569 (-) 624 WP_060739559.1 type 4a pilus biogenesis protein PilO Machinery gene
  AO356_RS09425 (AO356_09410) - 2130566..2131132 (-) 567 WP_060739560.1 PilN domain-containing protein -
  AO356_RS09430 (AO356_09415) comM 2131132..2132196 (-) 1065 WP_060739561.1 pilus assembly protein PilM Machinery gene
  AO356_RS09435 (AO356_09420) - 2132405..2134849 (+) 2445 WP_203225789.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 23087.41 Da        Isoelectric Point: 4.3966

>NTDB_id=136714 AO356_RS09420 WP_060739559.1 2129946..2130569(-) (pilO) [Pseudomonas fluorescens strain FW300-N2C3]
MRPGEWLDALRKIDVSDLDTNNIGSWPAPIKWLAGILLVALVLGLGYNFALSDLENQLQQVREEESTLKAQFAGKAHMAA
NLERYTEQMKQMETSFGVLLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVTQPFYIELPIQITVTGGYHDLATF
VSGVAGLPRIVTLHDFEIAPMEPEAGTKLRMSIQAKTYRYNEQEAQP

Nucleotide


Download         Length: 624 bp        

>NTDB_id=136714 AO356_RS09420 WP_060739559.1 2129946..2130569(-) (pilO) [Pseudomonas fluorescens strain FW300-N2C3]
ATGAGGCCGGGGGAATGGCTCGACGCGTTGCGCAAGATCGACGTCAGTGACCTGGACACCAACAACATCGGTTCCTGGCC
GGCGCCCATCAAATGGCTGGCGGGCATTCTGCTGGTGGCCCTGGTGCTGGGGCTGGGCTATAACTTTGCCTTGAGCGACC
TGGAAAACCAGTTGCAGCAGGTGCGCGAGGAAGAAAGTACCCTCAAGGCACAATTCGCGGGCAAGGCCCACATGGCCGCG
AACCTGGAACGCTACACCGAGCAGATGAAGCAGATGGAAACGTCTTTCGGTGTACTGTTGCGGCAATTGCCCAGTGATAC
CGAGGTGCCGGGCCTGCTGGAAGACATTACCCGCACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTGC
CGGAAGTCACCCAACCGTTCTACATCGAGCTGCCGATCCAGATCACCGTGACCGGCGGCTACCACGACCTGGCGACATTC
GTCAGTGGAGTGGCCGGACTGCCCCGGATCGTCACGCTGCACGATTTCGAAATCGCCCCGATGGAGCCCGAGGCCGGCAC
GAAGCTGCGCATGAGCATCCAGGCCAAGACCTACCGCTACAACGAGCAGGAGGCGCAGCCATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

42.347

94.686

0.401
  pilO Acinetobacter baumannii D1279779

43.085

90.821

0.391
  comO/pilO Acinetobacter baylyi ADP1

43.548

89.855

0.391