Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   GBS85147_RS01825 Genome accession   NZ_CP010319
Coordinates   330252..331541 (+) Length   429 a.a.
NCBI ID   WP_000432963.1    Uniprot ID   -
Organism   Streptococcus agalactiae strain GBS85147     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 325252..336541
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GBS85147_RS01805 (GBS85147_0366) - 326559..327227 (+) 669 WP_000394892.1 fructose-6-phosphate aldolase -
  GBS85147_RS01810 (GBS85147_0367) - 327295..328383 (+) 1089 WP_017647121.1 glycerol dehydrogenase -
  GBS85147_RS01815 (GBS85147_0368) cysK 328535..329461 (-) 927 WP_000036946.1 cysteine synthase A -
  GBS85147_RS01820 (GBS85147_0369) - 329552..330196 (-) 645 WP_001108150.1 YigZ family protein -
  GBS85147_RS01825 (GBS85147_0370) comFA/cflA 330252..331541 (+) 1290 WP_000432963.1 DEAD/DEAH box helicase Machinery gene
  GBS85147_RS01830 (GBS85147_0371) - 331541..332206 (+) 666 WP_017647122.1 ComF family protein -
  GBS85147_RS01835 (GBS85147_0372) hpf 332283..332837 (+) 555 WP_000599096.1 ribosome hibernation-promoting factor, HPF/YfiA family -

Sequence

Protein


Download         Length: 429 a.a.        Molecular weight: 48689.51 Da        Isoelectric Point: 9.9941

>NTDB_id=136357 GBS85147_RS01825 WP_000432963.1 330252..331541(+) (comFA/cflA) [Streptococcus agalactiae strain GBS85147]
MENYLGRLWTKAQLSEQLRKIAISLPSFIKKGSDYICTRCSSSVAKNCQLPTGNYYCRECIVFGRVTSNENLYYFPQKTF
SKTNSLKWKGGLTPYQNEVSEELLKGISSKENLLVHAVTGAGKTEMIYHSVAKVIDTGGSVCIASPRIDVCLELHKRLSN
DFRCAITLMHGESPSYQRSPLTIATTHQLLKFYHAFDLLIVDEVDAFPYVDNPILYHGVKQALKENGTSIFLTATSTTEL
ERKVARKELKKLHLARRFHANPLVIPEMVWVSGIQKSLQTQKLPPKLYQLINKQRQTRYPLLLFFPHISEGQVFTEMLRQ
AFPMEKIGFVSSKSTSRLKLVQDFRDNKLSILVSTTILERGVTFPSVDVFVIQANHHLFTKSSLVQISGRVGRALERPEG
LLYFLHDGKSKSMHQAIKEIKNMNHIGGF

Nucleotide


Download         Length: 1290 bp        

>NTDB_id=136357 GBS85147_RS01825 WP_000432963.1 330252..331541(+) (comFA/cflA) [Streptococcus agalactiae strain GBS85147]
ATGGAAAATTATTTAGGTAGACTTTGGACAAAAGCACAATTATCTGAGCAACTTAGAAAAATTGCAATTAGCTTGCCATC
TTTTATAAAGAAAGGAAGCGACTATATTTGTACCCGATGCTCTAGTTCTGTTGCCAAAAATTGCCAATTACCAACTGGAA
ATTATTATTGTAGAGAATGTATAGTTTTTGGAAGAGTTACTAGTAATGAAAATTTATATTATTTCCCACAAAAAACATTT
TCTAAAACGAATTCTTTAAAATGGAAAGGGGGACTGACACCTTATCAAAATGAGGTTTCAGAAGAGTTGTTGAAAGGTAT
TTCAAGTAAGGAAAATCTTCTTGTTCATGCTGTGACAGGGGCTGGTAAAACAGAAATGATATACCACTCTGTCGCCAAAG
TAATTGATACTGGAGGAAGCGTTTGTATTGCCAGTCCTAGAATTGATGTTTGTTTAGAATTGCATAAGCGACTTTCAAAC
GATTTTAGATGTGCCATAACATTAATGCATGGAGAATCTCCTTCTTATCAGAGATCCCCTTTAACTATTGCCACAACACA
TCAATTGTTAAAATTTTATCATGCTTTTGATTTGCTAATTGTTGATGAAGTTGATGCTTTTCCATATGTCGACAACCCGA
TACTATACCATGGTGTAAAACAGGCTTTAAAGGAAAACGGAACAAGTATTTTTCTGACTGCAACTTCAACGACTGAGTTA
GAAAGAAAAGTTGCAAGAAAAGAACTAAAAAAATTACATCTAGCTAGGAGATTTCATGCCAATCCGTTAGTGATACCCGA
GATGGTTTGGGTAAGTGGTATCCAGAAAAGCTTGCAGACACAAAAACTACCTCCCAAATTATATCAGCTGATAAATAAAC
AGCGACAAACGAGATATCCTTTGTTATTATTTTTCCCCCATATTTCAGAAGGTCAGGTATTCACCGAAATGTTAAGACAA
GCCTTTCCTATGGAAAAAATTGGTTTTGTTTCAAGTAAGAGCACATCTAGGTTAAAGTTAGTGCAAGATTTTCGAGATAA
CAAATTGTCTATCCTAGTGTCAACTACCATTCTAGAGAGAGGAGTAACTTTCCCTAGCGTCGACGTTTTTGTTATACAAG
CAAATCATCATTTGTTTACGAAAAGTTCCTTGGTACAAATTTCGGGAAGAGTTGGAAGAGCATTAGAAAGGCCCGAAGGG
CTCCTTTACTTCCTTCATGATGGGAAAAGTAAATCGATGCACCAAGCTATAAAGGAGATTAAAAACATGAATCATATAGG
AGGATTTTAA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(286-394)

DEAD/DEAH box helicase (DEAD)

(93-240)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis NCTC 12261

60.656

99.534

0.604
  comFA/cflA Streptococcus pneumoniae Rx1

60.187

99.534

0.599
  comFA/cflA Streptococcus pneumoniae D39

60.187

99.534

0.599
  comFA/cflA Streptococcus pneumoniae R6

60.187

99.534

0.599
  comFA/cflA Streptococcus mitis SK321

60.187

99.534

0.599
  comFA/cflA Streptococcus pneumoniae TIGR4

60.187

99.534

0.599
  comFA Lactococcus lactis subsp. cremoris KW2

50

91.841

0.459
  comFA Bacillus subtilis subsp. subtilis str. 168

39.066

94.872

0.371
  comFA Latilactobacillus sakei subsp. sakei 23K

36.322

100

0.368

Multiple sequence alignment