Detailed information NTG0136288

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: RP72_RS13830
• Genome accession: NZ_CP010314
• Coordinates: 2620006..2620623 (-)
• Length: 205 a.a.
• NCBI ID: WP_004398514.1
• Uniprot ID: A0AAE2SLR2
• Organism: Bacillus subtilis subsp. subtilis strain 3NA isolate 1970 (Michel and Millet)
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2615006..2625623

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RP72_RS13805 (RP72_13805)	rpsT	2615308..2615574 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
RP72_RS13810 (RP72_13810)	holA	2615589..2616632 (-)	1044	WP_003229987.1	DNA polymerase III subunit delta	-
RP72_RS23345	-	2616672..2616821 (-)	150	WP_003229985.1	hypothetical protein	-
RP72_RS22755 (RP72_13815)	yqzM	2616862..2616996 (+)	135	WP_003229983.1	YqzM family protein	-
RP72_RS13820 (RP72_13820)	comEC	2617036..2619366 (-)	2331	WP_009967776.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
RP72_RS13825 (RP72_13825)	comEB	2619370..2619939 (-)	570	WP_003229978.1	ComE operon protein 2	-
RP72_RS13830 (RP72_13830)	comEA	2620006..2620623 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
RP72_RS13835 (RP72_13835)	comER	2620707..2621528 (+)	822	WP_004398597.1	late competence protein ComER	-
RP72_RS13840 (RP72_13840)	yqeM	2621594..2622337 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
RP72_RS13845 (RP72_13845)	rsfS	2622334..2622690 (-)	357	WP_003229971.1	ribosome silencing factor	-
RP72_RS13850 (RP72_13850)	yqeK	2622708..2623268 (-)	561	WP_004399059.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
RP72_RS13855 (RP72_13855)	nadD	2623258..2623827 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
RP72_RS13860 (RP72_13860)	yhbY	2623839..2624129 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
RP72_RS13865 (RP72_13865)	aroE	2624123..2624965 (-)	843	WP_003229967.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21769.45 Da
• Isoelectric Point: 4.7220

>NTDB_id=136288 RP72_RS13830 WP_004398514.1 2620006..2620623(-) (comEA) [Bacillus subtilis subsp. subtilis strain 3NA isolate 1970 (Michel and Millet)]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=136288 RP72_RS13830 WP_004398514.1 2620006..2620623(-) (comEA) [Bacillus subtilis subsp. subtilis strain 3NA isolate 1970 (Michel and Millet)]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39