Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   AM599_RS27465 Genome accession   NZ_CP012585
Coordinates   5981974..5982597 (-) Length   207 a.a.
NCBI ID   WP_003116135.1    Uniprot ID   A0A0H2ZIF6
Organism   Pseudomonas aeruginosa strain PA_D1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5976974..5987597
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AM599_RS27445 (AM599_27445) aroB 5977581..5978687 (-) 1107 WP_069376936.1 3-dehydroquinate synthase -
  AM599_RS27450 (AM599_27450) aroK 5978737..5979255 (-) 519 WP_069376937.1 shikimate kinase AroK -
  AM599_RS27455 (AM599_27455) pilQ 5979267..5981399 (-) 2133 WP_069376938.1 type 4a pilus secretin PilQ Machinery gene
  AM599_RS27460 (AM599_27460) comL/pilP 5981453..5981977 (-) 525 WP_003116136.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  AM599_RS27465 (AM599_27465) pilO 5981974..5982597 (-) 624 WP_003116135.1 type 4a pilus biogenesis protein PilO Machinery gene
  AM599_RS27470 (AM599_27470) pilN 5982594..5983190 (-) 597 WP_003141783.1 type 4a pilus biogenesis protein PilN -
  AM599_RS27475 (AM599_27475) pilM 5983190..5984254 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  AM599_RS27480 (AM599_27480) - 5984439..5986910 (+) 2472 WP_043088268.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22958.35 Da        Isoelectric Point: 4.9107

>NTDB_id=134660 AM599_RS27465 WP_003116135.1 5981974..5982597(-) (pilO) [Pseudomonas aeruginosa strain PA_D1]
MSLASSLESLRKIDVNDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLRDLQTQLEQQAAQEESLKQQFATKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVTQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPASPDNTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=134660 AM599_RS27465 WP_003116135.1 5981974..5982597(-) (pilO) [Pseudomonas aeruginosa strain PA_D1]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGACGTAAACGACCTCGATCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTCCGTGACT
TGCAAACACAGCTCGAACAGCAGGCTGCGCAAGAAGAATCGCTCAAGCAGCAATTTGCTACCAAAGCGTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAGGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGTCTGGGCAGCGGCCTGGAGTTCGAGGAGATCAAGCTGCTCC
CTGAAGTCACTCAGCAGTTCTATATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTTACCCTGCATGACTTCGAGATCAAGCCGGCTTCGCCCGATAACACCTC
GAAGCTGCGCATGAGCATCTTGGCCAAGACCTACCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZIF6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

45.596

93.237

0.425
  pilO Acinetobacter baumannii D1279779

45.789

91.787

0.42
  comO/pilO Acinetobacter baylyi ADP1

43.617

90.821

0.396