Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   SAGV69_RS04495 Genome accession   NZ_CP009681
Coordinates   865530..866027 (+) Length   165 a.a.
NCBI ID   WP_001803317.1    Uniprot ID   A0A2I7Y542
Organism   Staphylococcus aureus strain Gv69     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 860530..871027
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SAGV69_RS04455 (SAGV69_00898) - 860574..861560 (+) 987 WP_000161314.1 ROK family glucokinase -
  SAGV69_RS04460 (SAGV69_00899) - 861560..861889 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  SAGV69_RS04465 (SAGV69_00900) - 861886..862509 (+) 624 WP_042857058.1 MBL fold metallo-hydrolase -
  SAGV69_RS04470 (SAGV69_00901) comGA 862561..863535 (+) 975 WP_000697228.1 competence type IV pilus ATPase ComGA Machinery gene
  SAGV69_RS04475 (SAGV69_00902) comGB 863507..864577 (+) 1071 WP_000775708.1 competence type IV pilus assembly protein ComGB Machinery gene
  SAGV69_RS04480 (SAGV69_00903) comGC 864591..864902 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  SAGV69_RS04485 (SAGV69_00904) comGD 864880..865326 (+) 447 WP_001790850.1 competence type IV pilus minor pilin ComGD Machinery gene
  SAGV69_RS04490 (SAGV69_03578) comGE 865313..865612 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  SAGV69_RS04495 (SAGV69_03579) comGF 865530..866027 (+) 498 WP_001803317.1 competence type IV pilus minor pilin ComGF Machinery gene
  SAGV69_RS04500 - 866124..866270 (+) 147 WP_001789879.1 hypothetical protein -
  SAGV69_RS04505 (SAGV69_00907) - 866260..866784 (+) 525 WP_001015117.1 shikimate kinase -
  SAGV69_RS04510 (SAGV69_00908) gcvT 866943..868034 (+) 1092 WP_000093351.1 glycine cleavage system aminomethyltransferase GcvT -
  SAGV69_RS04515 (SAGV69_00909) gcvPA 868054..869400 (+) 1347 WP_042857059.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  SAGV69_RS04520 (SAGV69_00910) gcvPB 869393..870865 (+) 1473 WP_000202189.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19212.92 Da        Isoelectric Point: 10.2192

>NTDB_id=131134 SAGV69_RS04495 WP_001803317.1 865530..866027(+) (comGF) [Staphylococcus aureus strain Gv69]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMFISITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDI
LDDFKGVDRNDIEIRQHRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=131134 SAGV69_RS04495 WP_001803317.1 865530..866027(+) (comGF) [Staphylococcus aureus strain Gv69]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGTTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGTAAAACTTTTCTAATTGAAAGTAGGGATTTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGATGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACACCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATTAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(67-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2I7Y542

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

99.394

100

0.994
  comGF Staphylococcus aureus N315

95.758

100

0.958

Multiple sequence alignment