Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   NG74_RS16605 Genome accession   NZ_CP009679
Coordinates   3460749..3462134 (-) Length   461 a.a.
NCBI ID   WP_022553867.1    Uniprot ID   -
Organism   Bacillus velezensis strain JS25R     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3455749..3467134
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NG74_RS16570 (NG74_03431) flgL 3455924..3456841 (-) 918 WP_007407477.1 flagellar hook-associated protein FlgL -
  NG74_RS16575 (NG74_03432) flgK 3456853..3458370 (-) 1518 WP_014419008.1 flagellar hook-associated protein FlgK -
  NG74_RS16580 (NG74_03433) - 3458386..3458868 (-) 483 WP_022553865.1 flagellar protein FlgN -
  NG74_RS16585 (NG74_03434) flgM 3458883..3459149 (-) 267 WP_003151393.1 flagellar biosynthesis anti-sigma factor FlgM -
  NG74_RS16590 (NG74_03435) - 3459219..3459638 (-) 420 WP_014419009.1 TIGR03826 family flagellar region protein -
  NG74_RS16595 (NG74_03436) comFC 3459712..3460401 (-) 690 WP_197538213.1 ComF family protein Machinery gene
  NG74_RS16600 (NG74_03437) - 3460407..3460691 (-) 285 WP_022553866.1 late competence development ComFB family protein -
  NG74_RS16605 (NG74_03438) comFA 3460749..3462134 (-) 1386 WP_022553867.1 DEAD/DEAH box helicase Machinery gene
  NG74_RS16610 (NG74_03439) - 3462242..3463090 (-) 849 WP_003151387.1 DegV family protein -
  NG74_RS16615 (NG74_03440) degU 3463188..3463877 (-) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  NG74_RS16620 (NG74_03441) degS 3463954..3465117 (-) 1164 WP_007407484.1 two-component sensor histidine kinase DegS Regulator
  NG74_RS16625 (NG74_03442) - 3465340..3465987 (+) 648 WP_007407485.1 YigZ family protein -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52705.20 Da        Isoelectric Point: 10.3124

>NTDB_id=131104 NG74_RS16605 WP_022553867.1 3460749..3462134(-) (comFA) [Bacillus velezensis strain JS25R]
MSEGASEFSEVRDFFYGRHLLRSEIPFSDQRIKRFTEKEYITAEPSIIRRKNRYVCQRCGQSDQASFAAFWAPSAKRQIT
YCRACVMMGRADELTSLYSWNQTPENSWEPVKLSWEGTLTDGQKRAAAALTDAIKERQELLVWAVCGSGKTEMLFPGIEF
ALNHGLRVCVATPRTDVVLELLPRLKKAFEKVEVSALYGGSEDKGRLTPLMISTAHQLMRYRDIFDVMIIDEVDAFPFSA
DETLRFAVDKARKKNSALVYVTATPSDTLKKKAEAGLLKSVRIPARYHRKPLPEPRFLWCGNWKKKLQKGKLPRSVTDWV
RQKLQLQLPVFLFVPSVHVLKKTTDYFRKLNIRAEGVHAEDTFRKDKVKRFRDGRLDLLVTTTILERGVTVPKVQTCVLG
AEAPIFTESVLVQIAGRTGRHYKHFSGDVVMFHFGITNGMKKAKKHIEHMNKLAQKSKLLD

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=131104 NG74_RS16605 WP_022553867.1 3460749..3462134(-) (comFA) [Bacillus velezensis strain JS25R]
ATGTCCGAAGGTGCTTCAGAATTTTCAGAGGTGCGGGATTTCTTTTACGGCCGGCATTTATTGAGATCTGAGATACCGTT
CTCAGACCAGCGTATCAAGCGTTTTACAGAAAAAGAATATATAACGGCCGAGCCGTCAATCATCCGCCGGAAAAACCGTT
ACGTCTGCCAACGATGCGGTCAGTCTGATCAAGCCAGCTTTGCGGCATTTTGGGCGCCCTCTGCCAAAAGGCAGATTACC
TATTGCCGTGCCTGTGTCATGATGGGAAGGGCTGATGAACTGACATCATTATATTCTTGGAATCAAACCCCTGAAAACAG
CTGGGAGCCTGTAAAGCTTTCTTGGGAAGGAACACTGACAGACGGACAGAAGCGGGCGGCCGCCGCGCTTACAGATGCGA
TAAAAGAGAGACAAGAGCTGTTAGTATGGGCGGTTTGCGGATCGGGGAAAACAGAGATGCTGTTTCCGGGTATCGAGTTT
GCGCTGAATCACGGTTTGCGCGTCTGTGTCGCGACTCCGCGTACAGATGTCGTACTTGAACTGCTGCCGAGACTGAAAAA
AGCATTTGAGAAAGTCGAAGTCTCTGCTTTGTACGGCGGCAGCGAAGACAAAGGCCGCCTGACTCCGCTGATGATTTCAA
CCGCTCACCAGCTGATGCGCTACCGAGACATTTTTGATGTCATGATAATAGATGAAGTAGATGCGTTTCCTTTTTCAGCC
GATGAGACATTAAGATTCGCTGTTGATAAAGCGAGGAAGAAAAACAGCGCCCTTGTTTATGTAACGGCGACTCCTTCAGA
CACATTAAAAAAGAAGGCTGAAGCCGGTTTGCTGAAAAGCGTCCGCATACCGGCCAGATATCATCGGAAACCCCTCCCGG
AGCCGCGTTTTTTGTGGTGCGGCAATTGGAAGAAGAAGCTTCAGAAAGGAAAACTGCCGCGCTCTGTAACGGATTGGGTC
CGCCAAAAACTTCAATTACAGCTGCCGGTTTTCTTATTCGTCCCGTCTGTACACGTATTGAAAAAGACAACGGATTATTT
TCGGAAATTAAACATACGGGCAGAAGGCGTACATGCGGAAGACACATTCAGAAAGGATAAAGTGAAACGATTCAGAGACG
GCCGGCTTGACCTGCTGGTAACGACTACGATTTTAGAAAGAGGGGTTACCGTGCCCAAAGTTCAGACATGTGTCCTCGGC
GCAGAAGCACCTATCTTTACTGAGAGCGTGCTCGTACAGATCGCGGGCAGAACGGGAAGGCATTATAAACATTTCAGCGG
TGATGTCGTCATGTTTCATTTCGGAATTACAAACGGAATGAAAAAAGCAAAAAAACATATAGAACATATGAACAAATTGG
CACAAAAAAGTAAATTGTTGGACTAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(330-421)

Type III restriction enzyme, res subunit (ResIII)

(118-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

62.391

99.783

0.623

Multiple sequence alignment