Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   NG74_RS05685 Genome accession   NZ_CP009679
Coordinates   1126919..1128748 (+) Length   609 a.a.
NCBI ID   WP_096145021.1    Uniprot ID   -
Organism   Bacillus velezensis strain JS25R     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 1121919..1133748
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NG74_RS05660 (NG74_01161) - 1122583..1123161 (+) 579 WP_007409110.1 GNAT family N-acetyltransferase -
  NG74_RS05665 (NG74_01162) spx 1123340..1123735 (+) 396 WP_003155034.1 transcriptional regulator Spx -
  NG74_RS05670 (NG74_01163) - 1123792..1124448 (-) 657 WP_014417421.1 TerC family protein -
  NG74_RS05675 (NG74_01164) mecA 1124724..1125380 (+) 657 WP_038461347.1 adaptor protein MecA Regulator
  NG74_RS05680 (NG74_01165) - 1125531..1126691 (+) 1161 WP_022553555.1 competence protein CoiA family protein -
  NG74_RS05685 (NG74_01166) pepF 1126919..1128748 (+) 1830 WP_096145021.1 oligoendopeptidase F Regulator
  NG74_RS20235 (NG74_01167) - 1128786..1128953 (-) 168 WP_003155026.1 hypothetical protein -
  NG74_RS05695 (NG74_01169) spxH 1129240..1130142 (-) 903 WP_003155024.1 protease adaptor protein SpxH -
  NG74_RS05700 (NG74_01170) - 1130139..1130537 (-) 399 WP_003155023.1 thiol management oxidoreductase -
  NG74_RS05705 (NG74_01171) - 1130766..1131452 (-) 687 WP_022553553.1 lytic transglycosylase domain-containing protein -
  NG74_RS05710 (NG74_01172) - 1131457..1132029 (-) 573 WP_021494986.1 CYTH domain-containing protein -
  NG74_RS05715 (NG74_01173) - 1132154..1132519 (+) 366 WP_012117290.1 hypothetical protein -
  NG74_RS05720 (NG74_01174) yjbM 1132547..1133182 (+) 636 WP_003155019.1 GTP diphosphokinase -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 69831.38 Da        Isoelectric Point: 5.1368

>NTDB_id=131064 NG74_RS05685 WP_096145021.1 1126919..1128748(+) (pepF) [Bacillus velezensis strain JS25R]
MTEENKAAQLPDRSEVKAEHTWRLEDIFPSDEAWNKEFQAVKELIPNLSSFKGTLAHSADNLYAALTAQDEVTNRLGKLY
AYAHMRSDQDTGNSFYQGLNDKAASLYSQAASATAYIEPEILAIQEDKLHQFILEKEELKLYSHAIEEITKQRPHVLSEQ
EEALLAEASEALSSPSNTFSVLNNADITFPSIKDENGKETQITHGNFINFLESSNREVRKNAFEAVYKTYGQYKNTMATT
LSGTVKKDNFYARVKNYKSAREAALSNNSIPEEVYDNLIKTINKHLPLLHRYIDLRKKVLGLDEVHIYDLYTPLVKDSGM
KVTYEEAKDYMLKGLAPLGEEYSSILKEGLENRWVDVYENKGKRSGAYSSGTYGTNPYILMNWHDNVNNLFTLVHEFGHS
VHSYYTRKYQPYPYGNYSIFVAEVASTTNEALLGEYMLNSLKDEKQRLYLLNHMLEGFRSTVFRQTMFAEFEHLIHTKAQ
EGEPLTPELLNELYYDLNKKYFGDNMVIDKEISLEWSRIPHFYYNYYVYQYATGYSAAQALSSQILKEGKPAVERYTEFL
KAGSSEYPIDILKKAGVDMTSPEPIEAACKMFEEKLNEMEELLSKVNPS

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=131064 NG74_RS05685 WP_096145021.1 1126919..1128748(+) (pepF) [Bacillus velezensis strain JS25R]
ATGACTGAGGAAAATAAAGCAGCACAGCTGCCTGACAGAAGTGAAGTAAAGGCAGAGCATACATGGAGACTAGAGGATAT
TTTTCCTAGTGATGAGGCCTGGAATAAAGAATTTCAAGCAGTTAAAGAATTAATTCCGAATTTATCTTCATTTAAAGGCA
CTCTTGCTCATTCGGCTGATAATTTATACGCGGCGCTTACGGCACAGGATGAAGTGACGAACCGCCTTGGGAAACTTTAT
GCATACGCACATATGCGCTCAGACCAGGATACGGGAAATTCTTTCTACCAAGGGCTGAACGACAAAGCCGCAAGCCTGTA
TTCCCAGGCTGCCAGCGCGACTGCTTACATTGAACCGGAAATTCTGGCGATTCAGGAGGACAAGCTGCACCAGTTCATTC
TGGAAAAAGAAGAGCTTAAGCTTTACTCCCATGCAATTGAAGAAATTACAAAACAGCGTCCGCACGTGCTGAGCGAACAA
GAAGAGGCCCTGCTTGCGGAAGCCTCAGAAGCGCTGTCGTCGCCTTCCAATACATTCAGTGTTTTAAATAACGCCGATAT
TACGTTTCCGTCGATTAAAGATGAAAACGGCAAGGAAACTCAAATCACACACGGCAACTTTATTAATTTCCTGGAAAGCA
GCAATCGTGAAGTCCGCAAAAACGCATTTGAAGCCGTATATAAAACATACGGACAGTATAAAAACACGATGGCGACTACA
CTCAGCGGCACTGTAAAAAAAGATAATTTTTACGCCCGTGTGAAAAACTATAAATCAGCACGGGAAGCGGCTTTATCCAA
TAACAGCATTCCTGAAGAAGTTTACGATAACCTTATCAAAACGATCAATAAACATCTTCCGTTATTACACCGTTACATTG
ACCTGCGGAAAAAGGTGCTCGGCCTTGATGAAGTCCATATTTATGACCTGTACACACCGCTTGTAAAAGATTCGGGAATG
AAGGTCACATATGAGGAAGCGAAAGATTATATGCTCAAAGGACTTGCTCCGCTGGGCGAGGAATACTCGTCTATTTTAAA
AGAAGGACTTGAAAACCGCTGGGTCGATGTATACGAAAACAAAGGAAAACGCAGCGGTGCTTATTCGTCCGGTACATACG
GCACGAATCCGTACATCTTAATGAACTGGCATGATAATGTCAATAATCTGTTTACGCTCGTTCACGAGTTCGGCCATTCC
GTGCACAGCTACTATACGAGAAAATATCAGCCGTATCCGTACGGTAACTACAGCATTTTTGTCGCTGAAGTCGCTTCAAC
GACTAATGAAGCGCTGCTCGGTGAATATATGCTGAACAGCTTAAAGGATGAAAAACAGCGTCTGTACCTGCTCAACCACA
TGCTTGAAGGATTCAGAAGCACAGTGTTCAGACAGACGATGTTCGCTGAATTTGAGCATCTGATTCATACGAAAGCGCAG
GAAGGCGAGCCGCTGACGCCTGAACTGCTGAACGAACTTTATTATGATCTGAATAAAAAGTATTTCGGTGACAACATGGT
GATTGATAAAGAAATCAGTCTGGAATGGTCAAGAATTCCGCATTTCTATTACAATTATTATGTGTATCAATATGCGACAG
GCTACAGTGCGGCCCAAGCGCTGAGCAGCCAGATTTTAAAAGAAGGCAAGCCTGCGGTGGAGCGCTATACCGAATTCTTA
AAAGCGGGAAGCTCGGAATATCCGATCGACATACTGAAAAAAGCGGGAGTCGATATGACATCGCCTGAACCGATCGAGGC
GGCCTGCAAAATGTTTGAAGAAAAACTGAATGAAATGGAAGAGCTTCTTTCGAAAGTGAACCCTTCGTAA

Domains

Predicted by InterproScan.

Oligopeptidase F (Peptidase_M3_N)

(120-189)

Peptidase family M3 (Peptidase_M3)

(211-590)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

50

97.537

0.488

Multiple sequence alignment