Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   BG07_RS08195 Genome accession   NZ_CP009651
Coordinates   1173699..1177424 (-) Length   1241 a.a.
NCBI ID   WP_040119299.1    Uniprot ID   -
Organism   Bacillus pseudomycoides strain BTZ     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1168699..1182424
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BG07_RS08145 (BG07_1180) - 1169323..1169829 (-) 507 WP_029427741.1 HXXEE domain-containing protein -
  BG07_RS08150 (BG07_1181) - 1169798..1170697 (-) 900 WP_018783050.1 fumarylacetoacetate hydrolase family protein -
  BG07_RS08155 (BG07_1182) - 1170939..1171118 (+) 180 WP_018783051.1 aspartyl-phosphate phosphatase Spo0E family protein -
  BG07_RS08160 (BG07_1183) - 1171254..1171475 (+) 222 WP_006093916.1 spore germination protein -
  BG07_RS08165 (BG07_1184) - 1171492..1171683 (+) 192 WP_006093915.1 spore germination protein GerPB -
  BG07_RS08170 (BG07_1185) - 1171767..1172381 (+) 615 WP_006093914.1 spore germination protein GerPC -
  BG07_RS08175 (BG07_1186) - 1172388..1172582 (+) 195 WP_018766557.1 hypothetical protein -
  BG07_RS08180 (BG07_1187) - 1172595..1172981 (+) 387 WP_006093913.1 spore germination protein GerPE -
  BG07_RS08185 (BG07_1188) gerPF 1173027..1173242 (+) 216 WP_003195867.1 spore germination protein GerPF -
  BG07_RS08190 (BG07_1189) - 1173328..1173621 (-) 294 WP_006093912.1 RNA polymerase alpha subunit C-terminal domain-containing protein -
  BG07_RS08195 (BG07_1190) addA 1173699..1177424 (-) 3726 WP_040119299.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  BG07_RS08200 (BG07_1191) addB 1177421..1180933 (-) 3513 WP_040119298.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  BG07_RS08205 (BG07_1192) lepB 1181059..1181622 (-) 564 WP_018783054.1 signal peptidase I -
  BG07_RS08210 (BG07_1193) - 1181681..1182274 (-) 594 WP_006093908.1 TVP38/TMEM64 family protein -

Sequence

Protein


Download         Length: 1241 a.a.        Molecular weight: 143291.71 Da        Isoelectric Point: 4.9577

>NTDB_id=130974 BG07_RS08195 WP_040119299.1 1173699..1177424(-) (addA) [Bacillus pseudomycoides strain BTZ]
MIENWPQKPEGSQWTDDQWKAVVRDGRDILVAAAAGSGKTAVLVERIIRKIINAENPVDVDRLLVVTFTNAAAQEMKNRI
GEALEKVLIDEPGSGHVRKQLSLLNKASISTIHSFCLQVIRTYYYMLDVDPRFRIANQTENELLKEEVLDDILEEEYGIE
GNDIFFELVDRYTSDRSDDDLQRMILALHTESRAHPDPEKWLDKLVEAYDVEGKSIEDLSYATYLLEDVKFQLETAEKHI
RKAIELAMLPDGPAPRIETLQADLVLLGTLSSAAHQSWTSVYEAMQNVSWQTLKRIKKSDYNEEIVKQVDSLRNKAKDEV
KKLQEELFSRKPESFLRDFKDMHPILEKLVQIVKVFTERFQTIKRDKGMVDFTDLEHFCLQILSERTNDGELRPSLVALQ
YRNKFAEVLVDEYQDTNFVQESIIKFVTKDFEQEGNLFMVGDVKQSIYRFRLAEPGLFLGKYKRFTPEGIDGGMKIDLAK
NFRSRHEVLAGTNFIFKQIMGETVGEIDYDADAELKLGANYPEGEDVAAELLCIHQSEGEESVEGEDGVEVEKAQLEARL
MAQRIKAMVDSGYEVYDRKTDSMRPVKYRDFVILLRSMPWAPQIMEELKLQGIPVYAELATGYFEATEVTVMMNVFRVID
NPMQDIPLAAVLRSPIVGLNDEELAMLRAHEKKGSFYEVMRSFLQGAPLEEEAELHGKLQWFYNLLQVWREFARQQSLSD
LIWKVYRETGYYDFVGGLPAGKQRQANLRVLYDRARQYEATSFRGLFRFLRFIERILERGDDMGTARALGEQEDVVRIMT
IHKSKGLEFPVVFVAGLGRRFNTQDLMKRFLLHKDFGFGSQFIDPRKRIKYTTISQLAIKRKMKMELIAEEMRVLYVALT
RAKEKLILIGTVKDKEKEIEKWLDSREHTDWLLPDYVRAGASCYLDWIAPSLYRHRDSEMLLELGQGNIPNEIYEYDASW
KVEVVDGSTLLAPEPVQEEKQELLEALREKKPVPLESERKEEVYARLTWEYAYEGATAHRAKQSVTEIKRNYQSEDGSDN
AFIKKLRTPIKTRPRFMEKKGLTYAERGTAVHAVMQHVNLQKPITLETVQEQIAEMVNKELLTFEQAEEIAIDRIVAFFD
TDLGKRVLAAKSVEREVPFTMMLSAEEAYQDWQNQSRESVLVQGVIDCMIEEEDGVVLIDFKTDTIEGKFSGGFDQAKPI
LEERYKVQLSLYAKALEKSLHHPVKEKCLYFFDGNHIVHVD

Nucleotide


Download         Length: 3726 bp        

>NTDB_id=130974 BG07_RS08195 WP_040119299.1 1173699..1177424(-) (addA) [Bacillus pseudomycoides strain BTZ]
ATGATAGAGAATTGGCCACAGAAGCCTGAAGGTAGTCAATGGACAGATGATCAATGGAAGGCAGTCGTCAGAGATGGCCG
TGATATTTTAGTAGCGGCGGCAGCGGGATCGGGAAAGACAGCTGTACTTGTAGAAAGAATTATTCGCAAAATTATTAATG
CTGAGAATCCAGTAGATGTGGATCGCTTGCTTGTCGTAACATTTACAAATGCAGCGGCACAAGAGATGAAAAACAGAATT
GGTGAAGCGTTAGAAAAAGTATTAATTGATGAGCCAGGATCAGGGCATGTTCGGAAACAGCTCAGCTTATTAAATAAAGC
ATCCATTTCAACCATTCACTCGTTCTGTTTACAAGTGATTCGAACGTACTATTATATGCTCGACGTTGATCCACGTTTTC
GCATTGCTAATCAGACGGAAAATGAATTGTTAAAAGAAGAAGTATTAGATGACATATTAGAAGAGGAATATGGAATCGAA
GGAAATGACATTTTCTTTGAACTAGTTGATCGATATACAAGCGATCGTAGTGATGATGACCTGCAGCGTATGATTTTAGC
GCTTCATACGGAATCGAGGGCACATCCAGATCCAGAGAAGTGGCTGGATAAGCTTGTCGAAGCATATGATGTGGAAGGGA
AATCAATTGAAGACTTATCGTATGCAACTTATTTATTAGAAGATGTGAAGTTTCAGCTGGAAACTGCTGAAAAGCATATT
CGAAAAGCAATTGAGTTAGCTATGCTTCCGGATGGGCCAGCGCCTCGCATTGAGACATTACAAGCGGACCTTGTGTTACT
CGGGACGTTATCTAGTGCAGCACATCAGTCTTGGACAAGTGTATATGAAGCGATGCAAAATGTTTCGTGGCAAACGTTAA
AGCGTATTAAAAAGAGTGATTACAATGAAGAGATTGTCAAACAAGTAGATTCACTTAGAAATAAAGCGAAAGATGAAGTG
AAGAAGCTACAAGAAGAGTTGTTTAGCCGGAAACCAGAAAGCTTTTTACGTGATTTCAAGGATATGCACCCTATACTAGA
AAAGCTTGTTCAGATTGTAAAAGTATTTACTGAGCGTTTTCAAACGATAAAGCGTGATAAAGGAATGGTTGATTTCACAG
ATTTAGAGCATTTTTGCTTACAAATTTTAAGTGAACGTACGAACGATGGTGAGCTTCGTCCATCACTAGTTGCGCTTCAA
TATCGGAATAAATTTGCCGAAGTACTTGTCGATGAATATCAAGATACGAACTTTGTGCAGGAGTCAATTATTAAGTTTGT
AACGAAAGACTTTGAACAAGAAGGTAATCTGTTTATGGTCGGCGACGTAAAACAGTCGATATACCGATTCCGATTAGCAG
AGCCTGGCTTATTTCTAGGAAAGTATAAACGTTTTACACCTGAAGGAATAGACGGCGGGATGAAGATTGATTTAGCCAAA
AACTTCCGCAGTCGTCATGAAGTACTAGCAGGTACGAACTTTATTTTTAAACAAATTATGGGCGAAACTGTCGGGGAAAT
CGATTACGATGCTGATGCTGAACTAAAGTTAGGTGCGAACTACCCAGAAGGAGAAGATGTAGCAGCCGAACTGTTATGTA
TTCATCAGTCTGAGGGAGAAGAATCGGTAGAGGGAGAAGACGGTGTAGAAGTAGAAAAAGCACAGCTTGAAGCACGTTTA
ATGGCGCAGCGAATTAAAGCGATGGTTGATTCAGGTTATGAAGTATATGATCGTAAAACAGATAGCATGCGCCCTGTGAA
ATACCGTGACTTTGTCATTTTACTTCGCTCGATGCCGTGGGCTCCGCAAATTATGGAAGAGTTAAAATTACAAGGCATTC
CTGTTTACGCTGAGCTCGCGACAGGTTATTTTGAAGCGACAGAAGTGACCGTTATGATGAATGTGTTTCGGGTAATTGAT
AATCCGATGCAAGATATTCCGCTTGCTGCTGTGCTTCGTTCCCCGATTGTTGGGTTAAATGATGAAGAGCTTGCGATGTT
ACGTGCTCATGAGAAAAAAGGTTCGTTTTATGAAGTGATGCGTTCCTTCTTACAAGGCGCGCCACTTGAAGAAGAGGCAG
AATTACATGGGAAATTACAATGGTTCTATAATCTCTTGCAAGTGTGGCGTGAATTTGCGCGCCAGCAGTCTCTTTCTGAT
TTAATTTGGAAAGTATATCGTGAGACGGGTTATTACGATTTCGTTGGTGGTCTTCCAGCGGGAAAACAGCGTCAAGCTAA
CTTACGCGTACTATATGATCGCGCAAGGCAGTACGAAGCAACATCTTTCCGCGGGTTATTCCGTTTCCTTCGCTTTATTG
AACGTATTTTAGAGCGCGGGGACGACATGGGAACAGCGAGAGCGCTTGGCGAACAAGAAGATGTGGTTCGAATTATGACG
ATTCATAAAAGTAAAGGGTTAGAGTTCCCAGTTGTATTTGTAGCGGGGCTTGGACGCCGTTTTAATACACAAGATTTAAT
GAAACGATTCTTATTACACAAAGATTTCGGTTTTGGATCACAGTTTATTGATCCTCGTAAACGAATTAAATATACAACGA
TATCACAACTAGCCATTAAACGTAAAATGAAGATGGAACTAATTGCGGAAGAGATGCGCGTATTATATGTTGCTCTAACG
CGTGCAAAAGAAAAACTAATTTTAATTGGTACGGTGAAAGATAAAGAAAAAGAAATTGAAAAGTGGCTTGACTCGCGTGA
ACATACAGATTGGTTGTTACCGGATTACGTTCGTGCAGGAGCATCTTGTTACTTAGATTGGATTGCACCGTCACTATATA
GACATCGTGATAGCGAAATGCTTCTTGAACTTGGACAAGGAAATATACCAAATGAAATTTATGAATACGATGCGAGCTGG
AAAGTGGAAGTGGTTGATGGTAGCACTTTACTTGCACCGGAACCAGTACAAGAAGAAAAGCAAGAATTGTTAGAAGCGCT
TCGTGAGAAAAAACCTGTTCCTCTAGAAAGTGAACGAAAAGAAGAGGTGTATGCTCGTTTAACTTGGGAATATGCGTATG
AAGGTGCAACAGCGCATCGGGCGAAGCAGTCTGTTACTGAGATAAAGCGGAATTATCAATCGGAAGATGGAAGTGATAAT
GCCTTTATTAAAAAACTTCGTACTCCTATAAAAACGCGTCCTCGTTTTATGGAGAAAAAGGGGTTAACTTATGCTGAACG
GGGGACAGCTGTTCACGCCGTAATGCAACATGTTAATTTACAAAAACCGATCACGCTTGAAACCGTTCAAGAGCAAATTG
CGGAAATGGTAAATAAAGAATTACTAACATTTGAGCAAGCAGAAGAAATTGCGATTGATCGAATTGTAGCATTTTTTGAT
ACGGATCTTGGTAAGCGTGTGTTAGCTGCGAAAAGTGTAGAACGTGAAGTGCCGTTTACGATGATGCTTTCAGCGGAAGA
AGCGTATCAAGATTGGCAAAATCAGAGCAGAGAATCTGTACTTGTCCAAGGGGTTATCGATTGTATGATAGAAGAGGAAG
ACGGAGTTGTATTAATCGATTTTAAAACAGATACAATTGAAGGAAAGTTCTCAGGTGGATTCGATCAAGCAAAGCCGATT
TTAGAAGAACGTTATAAAGTACAGCTTTCATTATATGCGAAAGCGTTAGAGAAAAGTTTACATCATCCAGTGAAAGAAAA
GTGTTTATATTTCTTTGATGGAAATCACATAGTACATGTTGATTAA

Domains

Predicted by InterproScan.

UvrD-like helicase C-terminal domain (UvrD_C)

(476-891)

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1062-1232)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(14-465)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

53.569

100

0.538

Multiple sequence alignment