Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   LT40_RS14235 Genome accession   NZ_CP009533
Coordinates   3216054..3217118 (-) Length   354 a.a.
NCBI ID   WP_043191355.1    Uniprot ID   A0A089YVV5
Organism   Pseudomonas rhizosphaerae strain DSM 16299     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3211054..3222118
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LT40_RS14210 (LT40_14465) aroK 3211642..3212160 (-) 519 WP_043191342.1 shikimate kinase AroK -
  LT40_RS14215 (LT40_14470) pilQ 3212165..3214267 (-) 2103 WP_043191344.1 type IV pilus secretin PilQ Machinery gene
  LT40_RS14220 (LT40_14475) - 3214304..3214831 (-) 528 WP_043191346.1 pilus assembly protein PilP -
  LT40_RS14225 (LT40_14480) pilO 3214828..3215487 (-) 660 WP_043191349.1 type 4a pilus biogenesis protein PilO -
  LT40_RS14230 (LT40_14485) - 3215484..3216053 (-) 570 WP_043191352.1 PilN domain-containing protein -
  LT40_RS14235 (LT40_14490) pilM 3216054..3217118 (-) 1065 WP_043191355.1 pilus assembly protein PilM Machinery gene
  LT40_RS14240 (LT40_14495) - 3217299..3219746 (+) 2448 WP_043191357.1 penicillin-binding protein 1A -
  LT40_RS14245 (LT40_14505) - 3220006..3220920 (+) 915 WP_043191366.1 Abi family protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37856.29 Da        Isoelectric Point: 4.8182

>NTDB_id=129892 LT40_RS14235 WP_043191355.1 3216054..3217118(-) (pilM) [Pseudomonas rhizosphaerae strain DSM 16299]
MRGLFSKKAHSLLGIDISSTSVKLLELSRTGNRFRVESYAVEPLPANAVVEKNIAELEGVGHALTRVLAKAKTGTRVVAV
AVAGSAVITKTIEMDAGLSDDELETQLKVEADQYIPYPLEEVAIDFEVQGYSARNPERVEVLLAACRKENVEVREAALAL
AGLSAKVVDVEAYALERAFGLLTAQLAGGNQALTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLSN
EEAGLAKKQGGLPDDYVAEVLQPFKDAVVQQVSRSLQFFFAAGQFNDVDYIMLAGGTASISGLDRLIQQRLGTPTLVANP
FADMALSSRVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=129892 LT40_RS14235 WP_043191355.1 3216054..3217118(-) (pilM) [Pseudomonas rhizosphaerae strain DSM 16299]
GTGCGTGGACTCTTCAGCAAGAAAGCACATTCGCTATTGGGCATCGATATCAGCTCCACGTCGGTCAAACTGCTCGAGCT
GAGCCGCACGGGCAACCGTTTTCGGGTCGAGTCGTATGCCGTGGAGCCGCTGCCGGCCAATGCCGTGGTCGAGAAGAACA
TCGCCGAGCTCGAGGGCGTAGGCCATGCCCTGACGCGCGTGCTGGCCAAGGCAAAAACCGGCACCCGCGTCGTGGCGGTG
GCGGTGGCCGGTTCGGCGGTCATCACCAAGACCATCGAGATGGACGCCGGGCTGTCCGACGACGAGCTCGAAACCCAGCT
CAAGGTCGAAGCCGACCAGTACATTCCCTATCCGCTGGAAGAAGTCGCCATCGACTTCGAGGTGCAGGGCTATTCGGCGC
GCAACCCGGAGCGGGTCGAGGTGCTGCTGGCCGCCTGTCGCAAGGAAAACGTCGAGGTCCGCGAGGCTGCGTTGGCTCTC
GCCGGGTTGAGCGCCAAGGTGGTCGACGTCGAGGCCTATGCGCTGGAGCGTGCCTTCGGCCTGCTCACCGCGCAGCTTGC
CGGCGGCAACCAGGCACTGACCGTGGCCGTGGTGGACATCGGTGCGACCATGACCACCCTCAGCGTGCTGCACAACGGCC
GCATCATCTATACCCGCGAGCAGTTGTTCGGCGGGCGTCAGCTGACCGAGGAAATCCAGCGCCGCTACGGCCTGTCCAAC
GAAGAAGCGGGCCTGGCCAAGAAGCAGGGCGGGTTGCCCGACGACTATGTGGCCGAAGTGCTGCAGCCGTTCAAGGATGC
CGTGGTGCAGCAGGTGTCGCGCTCGCTGCAGTTCTTCTTCGCTGCCGGCCAGTTCAACGATGTCGACTACATCATGCTCG
CCGGTGGCACCGCTTCCATTTCCGGCCTGGATCGGCTGATCCAGCAACGCCTGGGCACTCCGACCCTGGTGGCCAATCCG
TTCGCCGACATGGCCCTGAGCAGCCGGGTCAATGCCGGCGCCCTGGCCAGTGACGCACCGGCCCTGATGATCGCCTGTGG
CCTGGCGCTGAGGAGCTTCGACTAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A089YVV5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

58.192

100

0.582
  comM Acinetobacter nosocomialis M2

58.192

100

0.582
  comM Acinetobacter baylyi ADP1

54.52

100

0.545
  pilM Legionella pneumophila strain ERS1305867

46.045

100

0.46

Multiple sequence alignment