Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   LT40_RS14215 Genome accession   NZ_CP009533
Coordinates   3212165..3214267 (-) Length   700 a.a.
NCBI ID   WP_043191344.1    Uniprot ID   A0A089YQ07
Organism   Pseudomonas rhizosphaerae strain DSM 16299     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3207165..3219267
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LT40_RS14200 (LT40_14455) - 3208814..3210388 (-) 1575 WP_043191329.1 SPOR domain-containing protein -
  LT40_RS14205 (LT40_14460) aroB 3210444..3211547 (-) 1104 WP_043191340.1 3-dehydroquinate synthase -
  LT40_RS14210 (LT40_14465) aroK 3211642..3212160 (-) 519 WP_043191342.1 shikimate kinase AroK -
  LT40_RS14215 (LT40_14470) pilQ 3212165..3214267 (-) 2103 WP_043191344.1 type IV pilus secretin PilQ Machinery gene
  LT40_RS14220 (LT40_14475) - 3214304..3214831 (-) 528 WP_043191346.1 pilus assembly protein PilP -
  LT40_RS14225 (LT40_14480) pilO 3214828..3215487 (-) 660 WP_043191349.1 type 4a pilus biogenesis protein PilO -
  LT40_RS14230 (LT40_14485) - 3215484..3216053 (-) 570 WP_043191352.1 PilN domain-containing protein -
  LT40_RS14235 (LT40_14490) pilM 3216054..3217118 (-) 1065 WP_043191355.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 700 a.a.        Molecular weight: 75413.55 Da        Isoelectric Point: 4.9203

>NTDB_id=129891 LT40_RS14215 WP_043191344.1 3212165..3214267(-) (pilQ) [Pseudomonas rhizosphaerae strain DSM 16299]
MNRILSAFGISLGIAMLSPIVMAANLKTLDVASLPGDRVELKLSFDGPVTAPRGYTTEQPARIALDLPGVTSQLGARSRD
LGVGNARSVVVVEATDRTRIIVNLTKLAPYSTRVQGNDLFVLVGEGAASLQGTAIPAPTAARPAPRPAAAPTPARAAVPA
GRAIRNVDFQRGELGEGNVIIDLSDPTISPDIQEQGGKILLNFAKTQLPDPLRVRLDVKDFATPVQFVNSSVSATGATVT
IEPSGTFDYSSYQTENRLTISVRPLTNEDVARRNVERNVYTGDKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGIT
LRLQNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQRQIQELAPLRRELMQVNYAKAADIAKLFQSV
TRGENVETERGSITVDERTNNIIAYQTQDRLDELRRIVTQLDIPVRQVMIEARIVEANVGYNKALGVRWGGSTNLRGKNN
WTAYGLDNNGDEAGNTGADVGSNTPFIDLGAADATSGIGLGFVTNNVLLDLELSAMESTGNGEIVSQPKVVTSDKETAKI
LKGTEVPYQEASSSGATTVSFKEASLSLEVTPQITPDSRIIMEVKVTKDEPDYLNAVLGVPPISKNEVNAKVLVNDGETI
VIGGVFSNTQSKTVDKVPFLGDVPYVGRLFRRDVVSEAKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2103 bp        

>NTDB_id=129891 LT40_RS14215 WP_043191344.1 3212165..3214267(-) (pilQ) [Pseudomonas rhizosphaerae strain DSM 16299]
ATGAACAGGATTCTCTCAGCCTTCGGTATTTCGCTAGGGATAGCGATGCTTTCACCGATCGTCATGGCCGCGAACTTGAA
GACGCTCGACGTTGCCTCCCTGCCCGGTGATCGCGTCGAGCTCAAGTTGTCGTTCGACGGTCCGGTTACCGCACCGCGCG
GGTACACCACCGAGCAACCGGCACGCATCGCCCTCGACCTGCCGGGTGTCACCAGCCAGCTGGGCGCGCGCAGCCGCGAC
CTGGGCGTGGGCAATGCCCGCAGCGTGGTGGTGGTCGAGGCCACCGACCGCACGCGGATCATCGTCAACCTGACCAAGCT
GGCGCCCTACAGCACCCGTGTGCAGGGCAACGACCTGTTCGTGCTGGTGGGCGAAGGTGCAGCCTCCCTGCAAGGCACTG
CCATACCGGCGCCGACTGCGGCCCGACCGGCGCCGCGACCTGCAGCCGCGCCAACTCCCGCGCGGGCGGCGGTGCCGGCG
GGACGCGCCATTCGCAATGTCGATTTCCAGCGCGGCGAGCTGGGCGAGGGCAATGTGATCATCGATCTCAGCGACCCGAC
CATCAGCCCCGACATTCAGGAGCAGGGCGGCAAGATCCTGCTCAATTTCGCCAAGACCCAACTGCCCGATCCCTTGCGCG
TGCGCCTGGACGTCAAGGATTTCGCGACCCCGGTGCAGTTCGTCAACTCGTCGGTCAGCGCTACGGGCGCGACGGTCACC
ATCGAACCTAGTGGCACCTTCGACTACTCCTCGTACCAGACCGAGAATCGCCTGACCATCAGCGTGCGGCCACTGACCAA
CGAGGACGTGGCGCGGCGCAATGTCGAGCGCAACGTCTATACCGGCGACAAGCTCTCGCTGAATTTCCAGGACATCGACG
TGCGTTCGGTGCTGCAGTTGATCGCCGATTTCACCAACCTGAACCTGGTGGCCAGCGACACGGTGCAGGGCGGGATTACC
CTGCGCCTGCAGAACGTCCCCTGGGACCAGGCGCTTGACCTGGTACTCAAGACCAAGGGCCTGGACAAGCGCAAGGTCGG
CAACGTGCTGCTGGTGGCCCCGGCCGACGAGATCGCCGCCCGCGAGCGCCAGGAGCTGGAGTCGCAGCGGCAGATCCAGG
AGCTTGCGCCCCTGCGCCGCGAGCTGATGCAGGTCAACTATGCCAAGGCCGCCGACATCGCCAAGCTGTTCCAGTCGGTG
ACCCGCGGTGAAAATGTCGAGACCGAGCGCGGCTCGATCACCGTCGACGAACGCACCAACAACATCATTGCCTACCAGAC
CCAGGACCGACTGGACGAGCTGCGGCGCATCGTCACCCAGCTGGACATCCCGGTGCGTCAGGTGATGATCGAGGCGCGCA
TCGTCGAGGCCAACGTGGGCTACAACAAGGCGCTCGGCGTGCGCTGGGGCGGTTCCACCAACTTGCGCGGCAAGAACAAC
TGGACCGCCTATGGCTTGGACAACAATGGCGACGAAGCCGGCAACACCGGTGCCGACGTGGGCAGCAACACACCCTTCAT
CGACCTGGGCGCCGCCGATGCGACCTCCGGCATCGGCCTGGGCTTCGTGACCAACAACGTCCTGCTCGACCTCGAGCTCA
GTGCCATGGAAAGCACCGGCAACGGCGAGATCGTCTCGCAGCCCAAGGTGGTGACCTCCGACAAGGAAACCGCGAAGATC
CTCAAGGGCACCGAGGTGCCGTACCAGGAGGCCAGCTCCAGCGGTGCCACCACGGTATCGTTCAAGGAGGCGTCGCTGTC
GCTCGAGGTGACGCCGCAGATCACCCCGGACAGCCGCATCATCATGGAAGTGAAGGTCACCAAGGATGAGCCCGACTACC
TGAACGCGGTGCTCGGCGTGCCGCCCATCAGCAAGAACGAGGTCAACGCCAAGGTGCTGGTCAACGACGGCGAAACCATC
GTCATCGGTGGGGTTTTCTCCAACACGCAAAGTAAAACCGTGGACAAGGTGCCCTTTTTGGGCGACGTGCCGTATGTTGG
GCGCCTTTTCCGTCGGGATGTCGTGTCGGAAGCCAAATCCGAGCTGCTGGTATTCTTGACACCGCGTATCATGAATAACC
AGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(38-121)

Secretin and TonB N terminus short domain (STN)

(308-354)

Bacterial type II and III secretion system protein (Secretin)

(535-691)

Bacterial type II/III secretion system short domain (Secretin_N)

(383-447)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A089YQ07

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.677

100

0.756
  comQ Acinetobacter baylyi ADP1

41.486

100

0.439
  pilQ Acinetobacter baumannii strain A118

38.338

100

0.409
  pilQ Acinetobacter baumannii D1279779

38.338

100

0.409
  pilQ Legionella pneumophila strain Lp02

39.27

97.857

0.384
  pilQ Legionella pneumophila strain ERS1305867

39.27

97.857

0.384

Multiple sequence alignment