Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   ACJ74_RS08010 Genome accession   NZ_CP011915
Coordinates   1574057..1574716 (-) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain PSUO2     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 1569057..1579716
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJ74_RS07980 (ACJ74_07700) ygiN 1569958..1570272 (-) 315 WP_000633738.1 putative quinol monooxygenase -
  ACJ74_RS07985 (ACJ74_07705) mdaB 1570303..1570884 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  ACJ74_RS26545 (ACJ74_07710) - 1571134..1571613 (+) 480 WP_000065329.1 Hcp family type VI secretion system effector -
  ACJ74_RS26550 (ACJ74_07715) - 1571616..1572326 (+) 711 WP_000834024.1 hypothetical protein -
  ACJ74_RS08000 (ACJ74_07720) ygiZ 1572333..1572665 (+) 333 WP_000914696.1 DUF2645 family protein -
  ACJ74_RS08005 (ACJ74_07725) qseC 1572711..1574060 (-) 1350 WP_000673367.1 quorum sensing histidine kinase QseC -
  ACJ74_RS08010 (ACJ74_07730) ciaR 1574057..1574716 (-) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  ACJ74_RS08015 (ACJ74_07735) ygiW 1574868..1575260 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  ACJ74_RS08020 (ACJ74_07740) ygiV 1575313..1575795 (+) 483 WP_000183508.1 AraC family transcriptional regulator -
  ACJ74_RS08025 (ACJ74_07745) ygiS 1575904..1577511 (+) 1608 WP_001518890.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=129812 ACJ74_RS08010 WP_001221493.1 1574057..1574716(-) (ciaR) [Escherichia coli strain PSUO2]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=129812 ACJ74_RS08010 WP_001221493.1 1574057..1574716(-) (ciaR) [Escherichia coli strain PSUO2]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAAGCGCTATATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCGGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTATTGATCCTGACTGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGCAACGTCATGC
TAGACCCTGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACGCTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment