Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   LG36_RS10535 Genome accession   NZ_CP009472
Coordinates   2193327..2194688 (-) Length   453 a.a.
NCBI ID   WP_003130563.1    Uniprot ID   -
Organism   Lactococcus lactis strain AI06     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 2195315..2208314 2193327..2194688 flank 627


Gene organization within MGE regions


Location: 2193327..2208314
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LG36_RS10535 (LG36_2013) radA 2193327..2194688 (-) 1362 WP_003130563.1 DNA repair protein RadA Machinery gene
  LG36_RS10545 (LG36_2014) rplQ 2195315..2195695 (-) 381 WP_003130558.1 50S ribosomal protein L17 -
  LG36_RS10550 (LG36_2015) - 2195714..2196652 (-) 939 WP_003130557.1 DNA-directed RNA polymerase subunit alpha -
  LG36_RS10555 (LG36_2016) rpsK 2196700..2197083 (-) 384 WP_010906297.1 30S ribosomal protein S11 -
  LG36_RS10560 (LG36_2017) rpsM 2197104..2197469 (-) 366 WP_003130555.1 30S ribosomal protein S13 -
  LG36_RS10565 rpmJ 2197487..2197603 (-) 117 WP_001808836.1 50S ribosomal protein L36 -
  LG36_RS10570 (LG36_2018) infA 2197657..2197875 (-) 219 WP_003130554.1 translation initiation factor IF-1 -
  LG36_RS10575 (LG36_2019) - 2198175..2198822 (-) 648 WP_010906298.1 adenylate kinase -
  LG36_RS10580 (LG36_2020) secY 2198962..2200281 (-) 1320 WP_003129919.1 preprotein translocase subunit SecY -
  LG36_RS10585 (LG36_2021) rplO 2200359..2200802 (-) 444 WP_003129920.1 50S ribosomal protein L15 -
  LG36_RS10590 (LG36_2022) rpmD 2201076..2201255 (-) 180 WP_003129921.1 50S ribosomal protein L30 -
  LG36_RS10595 (LG36_2023) rpsE 2201266..2201772 (-) 507 WP_003129922.1 30S ribosomal protein S5 -
  LG36_RS10600 (LG36_2024) rplR 2201791..2202138 (-) 348 WP_003129924.1 50S ribosomal protein L18 -
  LG36_RS10605 (LG36_2025) rplF 2202354..2202890 (-) 537 WP_003129925.1 50S ribosomal protein L6 -
  LG36_RS10610 (LG36_2026) rpsH 2203108..2203506 (-) 399 WP_003129932.1 30S ribosomal protein S8 -
  LG36_RS10615 (LG36_2027) - 2203721..2204353 (-) 633 WP_038602333.1 DUF6287 domain-containing protein -
  LG36_RS10620 (LG36_2028) - 2204504..2204689 (-) 186 WP_003129946.1 type Z 30S ribosomal protein S14 -
  LG36_RS10625 (LG36_2029) rplE 2204708..2205250 (-) 543 WP_003129948.1 50S ribosomal protein L5 -
  LG36_RS10630 (LG36_2030) rplX 2205270..2205575 (-) 306 WP_003129951.1 50S ribosomal protein L24 -
  LG36_RS10635 (LG36_2031) rplN 2205709..2206077 (-) 369 WP_003129953.1 50S ribosomal protein L14 -
  LG36_RS10640 (LG36_2032) rpsQ 2206100..2206360 (-) 261 WP_003129955.1 30S ribosomal protein S17 -
  LG36_RS10645 (LG36_2033) rpmC 2206381..2206590 (-) 210 WP_003129957.1 50S ribosomal protein L29 -
  LG36_RS10650 (LG36_2034) rplP 2206590..2207003 (-) 414 WP_003129958.1 50S ribosomal protein L16 -
  LG36_RS10655 (LG36_2035) rpsC 2207007..2207660 (-) 654 WP_003129960.1 30S ribosomal protein S3 -
  LG36_RS10660 (LG36_2036) rplV 2207673..2208020 (-) 348 WP_010906302.1 50S ribosomal protein L22 -
  LG36_RS10665 (LG36_2037) rpsS 2208036..2208314 (-) 279 WP_003129963.1 30S ribosomal protein S19 -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49893.28 Da        Isoelectric Point: 5.1748

>NTDB_id=129656 LG36_RS10535 WP_003130563.1 2193327..2194688(-) (radA) [Lactococcus lactis strain AI06]
MAKKKSSFVCQNCGYKSAKYLGRCPNCGEWSSFVEEVEVQEVKNQRVSMTGERSKPLKLDEVELFDTPRIETDLDEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLASRGRVLYVSGEESAQQIKLRAERLGDIDRDFYLYAETNMQSIRAEVE
RLKPNFLIIDSIQTIMTPEIQSTQGSVSQVREVTGELMQIAKTNDIATFIVGHVTKEGQLAGPRMLEHMVDTVLYFEGER
NNTFRILRAVKNRFGSTNEIGIFEMQGHGLVEVTNPSEVFLEERLEGSTGSAIVCALEGTRPILVEIQALTTPTMFGNAK
RTTSGLDFNRVSLIMAVLEKRTGLLMQNQDAYLKSAGGVKLDEPAIDLAVAVAVASSYKELPTDARECFIGEIGLTGEIR
RVTRIEQRLNEAAKLGFKKVYAPKNSIVGIDIPEQIQVIGVTTLTECLKLVFG

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=129656 LG36_RS10535 WP_003130563.1 2193327..2194688(-) (radA) [Lactococcus lactis strain AI06]
ATGGCGAAGAAAAAATCATCATTTGTTTGTCAAAATTGTGGCTATAAGTCTGCAAAATACTTAGGACGTTGTCCAAACTG
TGGCGAATGGTCTTCTTTTGTTGAAGAAGTTGAAGTCCAAGAAGTTAAAAATCAACGTGTTTCAATGACTGGCGAACGTT
CAAAACCATTGAAGCTAGATGAAGTTGAATTATTTGATACTCCTCGGATTGAAACGGACTTAGATGAATTTAATCGTGTA
TTAGGAGGGGGTGTTGTTCCTGGTTCATTAGTTCTGATTGGGGGAGACCCTGGGATAGGTAAATCAACCTTACTTTTACA
GGTTTCTACTCAATTGGCTTCTAGAGGTCGTGTGCTTTATGTGAGTGGTGAAGAATCAGCCCAACAAATTAAACTAAGAG
CTGAGCGTTTGGGAGATATTGACCGAGACTTCTATCTCTATGCTGAAACAAATATGCAGTCGATTCGTGCAGAAGTTGAG
CGTTTAAAACCTAACTTCTTAATTATTGATTCCATTCAAACAATTATGACACCAGAAATTCAATCAACTCAAGGCTCAGT
AAGTCAAGTTCGTGAAGTGACTGGAGAATTGATGCAAATTGCTAAAACGAATGATATTGCGACCTTTATTGTTGGTCATG
TGACTAAAGAAGGTCAGCTGGCTGGGCCAAGGATGTTAGAGCATATGGTTGATACGGTGCTGTATTTTGAAGGAGAACGA
AATAATACATTTAGAATTCTTCGTGCTGTAAAAAACCGTTTTGGTTCAACTAATGAAATTGGAATTTTTGAGATGCAGGG
TCATGGCCTGGTCGAAGTGACTAATCCCTCAGAGGTCTTTTTAGAAGAACGTTTAGAGGGCTCAACTGGATCTGCAATTG
TTTGTGCACTTGAAGGAACACGGCCCATATTAGTTGAAATACAAGCACTGACGACACCGACAATGTTTGGAAATGCTAAA
AGGACGACCTCAGGCCTAGATTTTAACCGTGTAAGTTTAATTATGGCGGTGCTTGAGAAGAGAACGGGGCTTTTGATGCA
AAATCAAGATGCCTACCTCAAATCTGCAGGGGGAGTAAAATTGGATGAGCCAGCCATTGACCTCGCGGTTGCAGTTGCTG
TGGCTTCAAGTTATAAAGAACTTCCAACAGATGCGCGTGAATGTTTTATTGGTGAAATTGGTTTAACGGGTGAAATTCGT
CGGGTGACCCGAATTGAACAAAGATTAAATGAAGCTGCAAAACTAGGCTTTAAAAAAGTATATGCTCCAAAAAATTCAAT
TGTAGGGATTGATATTCCTGAACAGATTCAAGTGATTGGAGTGACAACTTTAACTGAATGTTTAAAACTGGTCTTTGGCT
AA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(70-218)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

81.236

100

0.812
  radA Streptococcus pneumoniae D39

81.236

100

0.812
  radA Streptococcus pneumoniae R6

81.236

100

0.812
  radA Streptococcus pneumoniae TIGR4

81.236

100

0.812
  radA Streptococcus mitis SK321

81.236

100

0.812
  radA Streptococcus mitis NCTC 12261

81.015

100

0.81
  radA Bacillus subtilis subsp. subtilis str. 168

61.488

100

0.62

Multiple sequence alignment