Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   IX91_RS13900 Genome accession   NZ_CP009354
Coordinates   3033073..3034068 (-) Length   331 a.a.
NCBI ID   WP_004743296.1    Uniprot ID   -
Organism   Vibrio tubiashii ATCC 19109     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3028073..3039068
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IX91_RS13875 (IX91_14160) aroK 3029050..3029568 (-) 519 WP_004743301.1 shikimate kinase AroK -
  IX91_RS13880 (IX91_14165) pilQ 3029745..3031439 (-) 1695 WP_050809758.1 type IV pilus secretin PilQ Machinery gene
  IX91_RS13885 (IX91_14170) pilP 3031453..3031956 (-) 504 WP_004743299.1 pilus assembly protein PilP Machinery gene
  IX91_RS13890 (IX91_14175) pilO 3031946..3032533 (-) 588 WP_004743298.1 type 4a pilus biogenesis protein PilO Machinery gene
  IX91_RS13895 (IX91_14180) pilN 3032520..3033083 (-) 564 WP_004743297.1 PilN domain-containing protein Machinery gene
  IX91_RS13900 (IX91_14185) pilM 3033073..3034068 (-) 996 WP_004743296.1 type IV pilus assembly protein PilM Machinery gene
  IX91_RS13905 (IX91_14190) - 3034209..3036728 (+) 2520 WP_004743295.1 penicillin-binding protein 1A -
  IX91_RS13910 (IX91_14195) oxyR 3036797..3037687 (-) 891 WP_004743294.1 DNA-binding transcriptional regulator OxyR -
  IX91_RS13915 (IX91_14200) - 3037860..3038591 (+) 732 WP_004743293.1 glutathione peroxidase -
  IX91_RS13920 (IX91_14205) - 3038659..3039006 (-) 348 WP_004743292.1 RidA family protein -

Sequence

Protein


Download         Length: 331 a.a.        Molecular weight: 36774.28 Da        Isoelectric Point: 6.8208

>NTDB_id=128874 IX91_RS13900 WP_004743296.1 3033073..3034068(-) (pilM) [Vibrio tubiashii ATCC 19109]
MGKSLVTGIEIGHHSIKAVVLKPNKGTFALVDYRELPIEAGIFSDNHMSNYQKIVKKLKELRKSLPRFSTKVALAIPDNA
VISKVLQIDSGLSDNEIEYAISDAFSHQTPFSIDELYLDYVPLTADRQSSQTHYQVYATKKELVDPRVEVFKSAAFQPVV
VDLRAHALVHIWQLAARQQVKADWLMIDVGFDHASLCMDLADKAPFCKDIVLAIHEKEVDSNLCLSLAQSREEFTAELLD
KIQRNLQLIASMHGKSVKGIWLSGVGATMPALANEISTRFAIECELLNPLTLFSTTSQQPSDNLLTHGHRFTSAAGIALR
ALTWLESRHAA

Nucleotide


Download         Length: 996 bp        

>NTDB_id=128874 IX91_RS13900 WP_004743296.1 3033073..3034068(-) (pilM) [Vibrio tubiashii ATCC 19109]
ATGGGTAAATCATTAGTTACAGGCATTGAAATTGGACACCACAGTATTAAAGCGGTGGTTCTCAAGCCAAACAAGGGCAC
ATTTGCTCTTGTCGACTATCGAGAATTGCCAATCGAAGCGGGCATTTTCTCTGATAACCATATGTCAAATTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTAAGGAAGAGTTTGCCGCGATTTAGTACGAAAGTTGCATTGGCGATACCAGATAATGCG
GTAATAAGTAAGGTATTACAGATAGATAGCGGGCTTAGCGACAATGAAATTGAGTATGCTATTTCTGACGCATTTTCCCA
TCAAACTCCGTTTTCAATTGATGAACTCTACTTAGATTATGTCCCGCTGACCGCGGATAGACAGTCATCGCAAACTCACT
ACCAAGTATACGCGACGAAAAAAGAGCTGGTTGACCCTAGAGTAGAAGTGTTCAAAAGCGCTGCCTTTCAACCCGTAGTG
GTTGATTTACGTGCCCATGCTTTAGTGCATATTTGGCAGCTTGCTGCACGACAACAGGTTAAAGCCGACTGGCTTATGAT
AGATGTTGGTTTTGACCACGCTTCGTTGTGTATGGACCTTGCTGATAAAGCGCCTTTTTGCAAAGACATCGTTTTGGCAA
TCCATGAAAAAGAGGTGGATTCAAACCTTTGCTTAAGTCTGGCGCAAAGCCGTGAAGAGTTCACCGCTGAGTTACTAGAT
AAAATCCAACGCAATTTACAGCTTATTGCTTCAATGCACGGCAAGAGTGTTAAAGGGATTTGGCTGTCAGGTGTAGGGGC
TACCATGCCTGCTCTTGCGAATGAAATCAGCACGCGCTTTGCCATTGAGTGTGAGCTTCTTAACCCGCTTACTCTTTTTT
CAACAACTAGCCAACAGCCATCCGATAATCTGTTGACCCACGGGCATAGGTTCACATCGGCGGCTGGGATTGCTCTGCGC
GCGCTCACTTGGTTGGAGAGTCGTCATGCTGCATAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(234-321)

Type IV pilus assembly protein PilM; (PilM_2)

(8-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

51.917

100

0.532
  pilM Vibrio campbellii strain DS40M4

41.108

100

0.426

Multiple sequence alignment