Detailed information NTG0001266

Experimentally validated

Overview

• Name: lpp1977
• Type: Machinery gene
• Locus tag: GCO85_RS10215
• Genome accession: NZ_CP048618
• Coordinates: 2268882..2269688 (-)
• Length: 268 a.a.
• NCBI ID: WP_061637208.1
• Uniprot ID: -
• Organism: Legionella pneumophila strain ERS1305867
• Function: DNA uptake DNA binding and uptake

Function

The mutant with the deletion of the entire operon (Δlpp1976–lpp1978) was unable to take up DNA, indicative of a defect in type IV pilus-mediated DNA import.

Genomic Context

Location: 2263882..2274688

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GCO85_RS10190 (GCO85_10200)	-	2264322..2264972 (-)	651	WP_027220528.1	lysoplasmalogenase	-
GCO85_RS10195 (GCO85_10205)	-	2265052..2265552 (-)	501	WP_027220529.1	hypothetical protein	-
GCO85_RS10200 (GCO85_10210)	-	2266044..2266904 (+)	861	WP_014844284.1	polysaccharide deacetylase family protein	-
GCO85_RS10205 (GCO85_10215)	-	2267022..2268218 (-)	1197	WP_011214245.1	murein transglycosylase A	-
GCO85_RS10210 (GCO85_10220)	lpp1976	2268328..2268885 (-)	558	WP_061637207.1	hypothetical protein	Machinery gene
GCO85_RS10215 (GCO85_10225)	lpp1977	2268882..2269688 (-)	807	WP_061637208.1	hypothetical protein	Machinery gene
GCO85_RS10220 (GCO85_10230)	lpp1978	2269627..2269941 (-)	315	WP_027220533.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	Machinery gene
GCO85_RS10225 (GCO85_10235)	hisC	2270101..2271210 (+)	1110	WP_027220534.1	histidinol-phosphate transaminase	-
GCO85_RS10230 (GCO85_10240)	-	2271247..2271588 (+)	342	WP_010947715.1	4a-hydroxytetrahydrobiopterin dehydratase	-
GCO85_RS10235 (GCO85_10245)	secF	2271766..2272683 (-)	918	WP_027220536.1	protein translocase subunit SecF	-
GCO85_RS10240 (GCO85_10250)	secD	2272696..2274552 (-)	1857	WP_027220537.1	protein translocase subunit SecD	-

Sequence

Protein

• Length: 268 a.a.
• Molecular weight: 31532.72 Da
• Isoelectric Point: 9.7469

>NTDB_id=1266 GCO85_RS10215 WP_061637208.1 2268882..2269688(-) (lpp1977) [Legionella pneumophila strain ERS1305867]
MLRGLKKASQLFAPMIWVLIEMGRQTGFSLSEMLISLFLASIITITLTQFYIRSKPQYLKAQERLEINFDLLWIGDLLRD
SIRKAGFTPCVSVEKLKTFDSRHAERKIINFQIENMPQVQLRINRMSDVYTEVTNFMSPTQIVIEESVISNIKHPLIISD
CRQGEVHTVSKIDKLRNGYQITLKKPLQFRYDEITYLGEWLEETWFTKQNEKGEKTLHYKLFQTEELSPFIHEFYVTRRL
IKQRSVLDFSIGSDKETLRKISVSVRNL

Nucleotide

• Length: 807 bp

>NTDB_id=1266 GCO85_RS10215 WP_061637208.1 2268882..2269688(-) (lpp1977) [Legionella pneumophila strain ERS1305867]
ATGTTACGTGGTTTAAAAAAAGCCAGTCAATTATTCGCTCCTATGATCTGGGTGCTAATTGAAATGGGAAGACAAACAGG
ATTTAGTTTATCTGAGATGCTAATTAGTCTTTTTCTGGCAAGTATTATAACGATAACCCTTACTCAGTTTTATATAAGAA
GCAAGCCTCAATACCTAAAGGCACAAGAACGGTTAGAAATTAATTTTGATTTGCTGTGGATTGGTGATTTACTAAGAGAC
AGTATTAGAAAGGCTGGATTTACTCCATGCGTAAGTGTCGAAAAATTAAAAACTTTTGATTCTCGACATGCAGAGAGGAA
AATAATTAATTTTCAAATTGAAAATATGCCACAAGTTCAACTTCGTATTAACAGAATGAGTGATGTTTATACCGAAGTGA
CAAATTTTATGAGCCCAACACAAATAGTGATTGAAGAGTCAGTAATATCAAATATAAAACACCCATTAATTATTTCTGAT
TGCCGGCAAGGAGAAGTACATACAGTTTCAAAAATAGATAAACTACGCAATGGATATCAAATTACATTAAAAAAACCACT
GCAGTTTCGATACGACGAAATTACTTACTTGGGAGAGTGGCTTGAAGAAACATGGTTTACTAAACAAAATGAAAAGGGTG
AAAAGACATTACATTACAAACTGTTTCAAACAGAAGAATTAAGTCCATTCATTCATGAATTTTATGTTACCAGACGTCTT
ATCAAACAGAGGTCAGTATTGGATTTTTCAATTGGTAGCGATAAGGAAACGCTACGTAAAATCAGTGTTTCGGTACGAAA
TTTATGA

Domains

No domain identified.

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
lpp1977	Legionella pneumophila str. Paris	99.254	100	0.993

References

• [1]: Léo Hardy et al. (2021) Transposon Insertion Sequencing in a Clinical Isolate of Legionella pneumophila Identifies Essential Genes and Determinants of Natural Transformation. Journal of Bacteriology 203(3):e00548-20. [PMID: 33168636]