Detailed information    

experimental Experimentally validated

Overview

Name   lpp1976   Type   Machinery gene
Locus tag   GCO85_RS10210 Genome accession   NZ_CP048618
Coordinates   2268328..2268885 (-) Length   185 a.a.
NCBI ID   WP_061637207.1    Uniprot ID   -
Organism   Legionella pneumophila strain ERS1305867     
Function   DNA uptake   
DNA binding and uptake

Function

The mutant with the deletion of the entire operon (Δlpp1976–lpp1978) was unable to take up DNA, indicative of a defect in type IV pilus-mediated DNA import.


Genomic Context

Location: 2263328..2273885
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GCO85_RS10185 (GCO85_10195) - 2263623..2264162 (-) 540 WP_061637206.1 hypothetical protein -
  GCO85_RS10190 (GCO85_10200) - 2264322..2264972 (-) 651 WP_027220528.1 lysoplasmalogenase -
  GCO85_RS10195 (GCO85_10205) - 2265052..2265552 (-) 501 WP_027220529.1 hypothetical protein -
  GCO85_RS10200 (GCO85_10210) - 2266044..2266904 (+) 861 WP_014844284.1 polysaccharide deacetylase family protein -
  GCO85_RS10205 (GCO85_10215) - 2267022..2268218 (-) 1197 WP_011214245.1 murein transglycosylase A -
  GCO85_RS10210 (GCO85_10220) lpp1976 2268328..2268885 (-) 558 WP_061637207.1 hypothetical protein Machinery gene
  GCO85_RS10215 (GCO85_10225) lpp1977 2268882..2269688 (-) 807 WP_061637208.1 hypothetical protein Machinery gene
  GCO85_RS10220 (GCO85_10230) lpp1978 2269627..2269941 (-) 315 WP_027220533.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  GCO85_RS10225 (GCO85_10235) hisC 2270101..2271210 (+) 1110 WP_027220534.1 histidinol-phosphate transaminase -
  GCO85_RS10230 (GCO85_10240) - 2271247..2271588 (+) 342 WP_010947715.1 4a-hydroxytetrahydrobiopterin dehydratase -
  GCO85_RS10235 (GCO85_10245) secF 2271766..2272683 (-) 918 WP_027220536.1 protein translocase subunit SecF -

Sequence

Protein


Download         Length: 185 a.a.        Molecular weight: 21763.15 Da        Isoelectric Point: 6.7381

>NTDB_id=1265 GCO85_RS10210 WP_061637207.1 2268328..2268885(-) (lpp1976) [Legionella pneumophila strain ERS1305867]
MKKTDSGFILLMTLCITFLMSLLVLASLQQIFLQYKALNSQETFHQNFYQLEHWMMRLAHSPLLYAGMDCYLQKDYGANK
IANELVNNKGCLLILNQRKYRYFIEDLNEFSCLVLNKDGQKYASHHFRFTVLQDEEHGEYTIMQLRFIKLGSNLSSCPEE
EIQVKEGVSSWRYLPDYKNFEILTG

Nucleotide


Download         Length: 558 bp        

>NTDB_id=1265 GCO85_RS10210 WP_061637207.1 2268328..2268885(-) (lpp1976) [Legionella pneumophila strain ERS1305867]
ATGAAAAAAACCGATTCCGGTTTCATTCTGTTAATGACGCTCTGTATAACTTTCTTGATGAGCTTATTGGTTTTGGCAAG
CCTCCAACAGATATTCTTGCAATACAAAGCACTCAATTCACAGGAGACATTTCATCAAAATTTCTATCAGTTGGAACATT
GGATGATGCGACTCGCTCATTCACCGTTATTGTATGCAGGTATGGATTGTTATCTGCAAAAGGATTATGGAGCTAATAAA
ATAGCAAACGAGTTAGTTAACAATAAAGGATGTTTGCTCATATTAAATCAGAGAAAATACAGATATTTTATTGAGGACCT
GAACGAATTTTCTTGTTTGGTTCTTAATAAGGATGGGCAAAAATATGCTTCTCACCATTTTAGATTCACCGTATTACAAG
ACGAGGAGCATGGCGAATATACTATCATGCAACTAAGGTTTATCAAATTGGGTAGCAATTTGTCCTCTTGTCCAGAGGAG
GAAATACAGGTGAAGGAAGGGGTAAGCAGCTGGAGATACTTGCCAGATTATAAAAATTTTGAAATATTAACTGGTTGA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  lpp1976 Legionella pneumophila str. Paris

98.919

100

0.989

Multiple sequence alignment    



References

[1] Léo Hardy et al. (2021) Transposon Insertion Sequencing in a Clinical Isolate of Legionella pneumophila Identifies Essential Genes and Determinants of Natural Transformation. Journal of Bacteriology 203(3):e00548-20. [PMID: 33168636]