Detailed information    

experimental Experimentally validated

Overview

Name   lpp1978   Type   Machinery gene
Locus tag   GCO85_RS10220 Genome accession   NZ_CP048618
Coordinates   2269627..2269941 (-) Length   104 a.a.
NCBI ID   WP_027220533.1    Uniprot ID   -
Organism   Legionella pneumophila strain ERS1305867     
Function   DNA uptake   
DNA binding and uptake

Function

The mutant with the deletion of the entire operon (Δlpp1976–lpp1978) was unable to take up DNA, indicative of a defect in type IV pilus-mediated DNA import.


Genomic Context

Location: 2264627..2274941
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GCO85_RS10195 (GCO85_10205) - 2265052..2265552 (-) 501 WP_027220529.1 hypothetical protein -
  GCO85_RS10200 (GCO85_10210) - 2266044..2266904 (+) 861 WP_014844284.1 polysaccharide deacetylase family protein -
  GCO85_RS10205 (GCO85_10215) - 2267022..2268218 (-) 1197 WP_011214245.1 murein transglycosylase A -
  GCO85_RS10210 (GCO85_10220) lpp1976 2268328..2268885 (-) 558 WP_061637207.1 hypothetical protein Machinery gene
  GCO85_RS10215 (GCO85_10225) lpp1977 2268882..2269688 (-) 807 WP_061637208.1 hypothetical protein Machinery gene
  GCO85_RS10220 (GCO85_10230) lpp1978 2269627..2269941 (-) 315 WP_027220533.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  GCO85_RS10225 (GCO85_10235) hisC 2270101..2271210 (+) 1110 WP_027220534.1 histidinol-phosphate transaminase -
  GCO85_RS10230 (GCO85_10240) - 2271247..2271588 (+) 342 WP_010947715.1 4a-hydroxytetrahydrobiopterin dehydratase -
  GCO85_RS10235 (GCO85_10245) secF 2271766..2272683 (-) 918 WP_027220536.1 protein translocase subunit SecF -
  GCO85_RS10240 (GCO85_10250) secD 2272696..2274552 (-) 1857 WP_027220537.1 protein translocase subunit SecD -
  GCO85_RS10245 (GCO85_10255) yajC 2274574..2274909 (-) 336 WP_010947718.1 preprotein translocase subunit YajC -

Sequence

Protein


Download         Length: 104 a.a.        Molecular weight: 12249.39 Da        Isoelectric Point: 10.0591

>NTDB_id=1267 GCO85_RS10220 WP_027220533.1 2269627..2269941(-) (lpp1978) [Legionella pneumophila strain ERS1305867]
MSKHKGFTLMEVLVSLMLMTTVVLMLVEQQLKTRDLINHLIWRIQGSQYLDQVDEALYISENRLPLPPSNYHFDIRRSQK
KIILNVTWFKKSQSIIRSYDLGAN

Nucleotide


Download         Length: 315 bp        

>NTDB_id=1267 GCO85_RS10220 WP_027220533.1 2269627..2269941(-) (lpp1978) [Legionella pneumophila strain ERS1305867]
ATGAGCAAGCATAAGGGTTTCACTCTAATGGAAGTACTTGTGTCATTAATGCTAATGACAACGGTAGTTCTGATGTTAGT
GGAACAACAGTTGAAAACCAGAGATCTAATCAATCATCTTATCTGGCGAATCCAAGGTTCCCAATATCTAGATCAAGTAG
ATGAAGCATTATATATTAGTGAGAATAGACTCCCTCTGCCGCCTTCAAATTATCATTTTGATATCAGGCGATCTCAAAAG
AAAATCATTTTGAATGTTACGTGGTTTAAAAAAAGCCAGTCAATTATTCGCTCCTATGATCTGGGTGCTAATTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  lpp1978 Legionella pneumophila str. Paris

98.077

100

0.981

Multiple sequence alignment    



References

[1] Léo Hardy et al. (2021) Transposon Insertion Sequencing in a Clinical Isolate of Legionella pneumophila Identifies Essential Genes and Determinants of Natural Transformation. Journal of Bacteriology 203(3):e00548-20. [PMID: 33168636]