Detailed information    

experimental Experimentally validated

Overview

Name   lpp1977   Type   Machinery gene
Locus tag   LPP_RS09970 Genome accession   NC_006368
Coordinates   2240332..2241138 (-) Length   268 a.a.
NCBI ID   WP_011214247.1    Uniprot ID   A0A3A6V0B9
Organism   Legionella pneumophila str. Paris     
Function   DNA uptake   
DNA binding and uptake

Function

The mutant with the deletion of the entire operon (Δlpp1976–lpp1978) was unable to take up DNA, indicative of a defect in type IV pilus-mediated DNA import.


Genomic Context

Location: 2235332..2246138
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LPP_RS09945 (lpp1972) - 2235773..2236423 (-) 651 WP_011214242.1 lysoplasmalogenase -
  LPP_RS09950 (lpp1973) - 2236503..2237003 (-) 501 WP_011214243.1 hypothetical protein -
  LPP_RS09955 (lpp1974) - 2237495..2238355 (+) 861 WP_011214244.1 polysaccharide deacetylase family protein -
  LPP_RS09960 (lpp1975) - 2238473..2239669 (-) 1197 WP_011214245.1 murein transglycosylase A -
  LPP_RS09965 (lpp1976) lpp1976 2239778..2240335 (-) 558 WP_011214246.1 hypothetical protein Machinery gene
  LPP_RS09970 (lpp1977) lpp1977 2240332..2241138 (-) 807 WP_011214247.1 PilW family protein Machinery gene
  LPP_RS09975 (lpp1978) lpp1978 2241077..2241391 (-) 315 WP_011214248.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  LPP_RS09980 (lpp1979) hisC 2241551..2242660 (+) 1110 WP_011214249.1 histidinol-phosphate transaminase -
  LPP_RS09985 (lpp1980) - 2242697..2243038 (+) 342 WP_010947715.1 4a-hydroxytetrahydrobiopterin dehydratase -
  LPP_RS09995 (lpp1981) secF 2243216..2244133 (-) 918 WP_010947716.1 protein translocase subunit SecF -
  LPP_RS10000 (lpp1982) secD 2244146..2246002 (-) 1857 WP_011214250.1 protein translocase subunit SecD -

Sequence

Protein


Download         Length: 268 a.a.        Molecular weight: 31491.67 Da        Isoelectric Point: 9.7469

>NTDB_id=1280 LPP_RS09970 WP_011214247.1 2240332..2241138(-) (lpp1977) [Legionella pneumophila str. Paris]
MLRGLKKASQLFAPMIWVLIEMGRQTGFSLSEMLISLFLASIITITLTQFYIRSKPQYLKAQERLEINFDLLWIGDLLRD
SIRKAGFTPCVSVEKLKTFDSRHAERKIINFQIENMPQVQLRINRMSDVYTEVTNFMSPTQIVIEESVISNIKHPLIISD
CRQGEVHTVSKIDKLSNGYQITLKKPLRFRYDEITYLGEWLEETWFTKQNEKGEKTLHYKLFQTEELSPFIHEFYVTRRL
IKQRSVLDFSIGSDKETLRKISVSVRNL

Nucleotide


Download         Length: 807 bp        

>NTDB_id=1280 LPP_RS09970 WP_011214247.1 2240332..2241138(-) (lpp1977) [Legionella pneumophila str. Paris]
ATGTTACGTGGTTTAAAAAAAGCCAGTCAATTATTCGCTCCTATGATCTGGGTGCTAATTGAAATGGGAAGACAAACAGG
ATTTAGTTTATCTGAGATGCTAATTAGTCTTTTTCTGGCAAGTATTATAACGATAACCCTTACTCAGTTTTATATAAGAA
GCAAGCCTCAATACCTAAAGGCACAAGAACGGTTAGAAATTAATTTTGATTTGCTGTGGATTGGTGATTTACTAAGAGAC
AGTATTAGAAAGGCTGGATTTACTCCATGCGTAAGTGTCGAAAAATTAAAAACTTTTGATTCTCGACATGCAGAGAGGAA
AATAATTAATTTTCAAATTGAAAATATGCCACAAGTTCAACTTCGTATTAACAGAATGAGTGATGTTTATACCGAAGTGA
CAAATTTTATGAGCCCAACACAAATAGTGATTGAAGAGTCAGTAATATCAAATATAAAACACCCATTAATTATTTCTGAT
TGCCGGCAAGGAGAAGTACATACAGTTTCAAAAATAGATAAACTAAGCAATGGATATCAAATTACTTTAAAAAAACCACT
GCGGTTTCGATACGACGAAATTACTTACTTGGGAGAGTGGCTTGAAGAAACATGGTTTACTAAACAAAATGAAAAGGGTG
AAAAGACATTACATTACAAACTGTTTCAAACAGAAGAATTAAGTCCATTCATTCATGAATTTTATGTTACCAGACGTCTT
ATCAAACAGAGGTCAGTATTGGATTTTTCAATTGGTAGCGATAAGGAAACGCTACGTAAAATCAGTGTTTCGGTACGAAA
TTTATGA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3A6V0B9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  lpp1977 Legionella pneumophila strain ERS1305867

99.254

100

0.993

Multiple sequence alignment    



References

[1] Léo Hardy et al. (2021) Transposon Insertion Sequencing in a Clinical Isolate of Legionella pneumophila Identifies Essential Genes and Determinants of Natural Transformation. Journal of Bacteriology 203(3):e00548-20. [PMID: 33168636]