Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AMD20_RS15255 Genome accession   NZ_CP009010
Coordinates   3407541..3408578 (-) Length   345 a.a.
NCBI ID   WP_003489919.1    Uniprot ID   -
Organism   Xanthomonas citri pv. citri strain JX5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3402541..3413578
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AMD20_RS15235 (J157_03085) - 3403110..3403682 (+) 573 WP_011051925.1 DNA-3-methyladenine glycosylase I -
  AMD20_RS15240 (J157_03086) - 3403690..3404616 (+) 927 WP_170913509.1 DUF72 domain-containing protein -
  AMD20_RS24925 - 3404907..3405158 (+) 252 WP_162010796.1 hypothetical protein -
  AMD20_RS15245 (J157_03088) - 3405194..3406198 (+) 1005 WP_005926112.1 pectate lyase -
  AMD20_RS15250 (J157_03089) pilU 3406288..3407418 (-) 1131 WP_003489921.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AMD20_RS15255 (J157_03090) pilT 3407541..3408578 (-) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  AMD20_RS15260 (J157_03091) - 3408990..3409682 (+) 693 WP_003489910.1 YggS family pyridoxal phosphate-dependent enzyme -
  AMD20_RS15265 (J157_03092) proC 3409726..3410580 (+) 855 WP_011051928.1 pyrroline-5-carboxylate reductase -
  AMD20_RS15270 (J157_03093) - 3411139..3411564 (+) 426 WP_003489904.1 HU family DNA-binding protein -
  AMD20_RS15275 (J157_03094) - 3411671..3412309 (+) 639 WP_015472435.1 M23 family metallopeptidase -
  AMD20_RS15280 (J157_03095) - 3412367..3412708 (+) 342 WP_011051930.1 DUF3861 family protein -
  AMD20_RS25640 (J157_03096) - 3412765..3413307 (+) 543 WP_005913301.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38643.30 Da        Isoelectric Point: 6.6652

>NTDB_id=126071 AMD20_RS15255 WP_003489919.1 3407541..3408578(-) (pilT) [Xanthomonas citri pv. citri strain JX5]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQHL
QDLVKRSLITRNQAREYAKDKRIFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=126071 AMD20_RS15255 WP_003489919.1 3407541..3408578(-) (pilT) [Xanthomonas citri pv. citri strain JX5]
ATGGATATCGCTGAACTATTGGCGTTTTCTGTCAAGAACAAGGCATCGGACCTGCACCTGTCTGCAGGGCTGCCGCCGAT
GATCCGTGTCGATGGCGATGTCCGCCGCATCAATATTCCGGCGTTGGACCACAAACAGGTGCACGCGCTGGTGTACGACA
TCATGTCGGACAAGCAGCGGCGCGATTACGAGGAATTCCTCGAAGTCGACTTTTCGTTCGAAATTCCGTCGCTGGCGCGT
TTTCGCGTCAATGCGTTCAACCAGAACCGCGGCGCCGGTGCGGTGTTCCGTACCATTCCGTCCGAAGTGCTGACGCTGGA
AGACCTTGGCTGCCCGCCGATCTTCCGTCAGCTGATCGATCAGCCGCAGGGCCTGATCCTGGTGACCGGCCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCCGGCATGATCGACTACATCAACAAGAATGAGTACGGGCACATCCTCACCGTCGAGGAC
CCGATCGAATTCGTCCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAGGTGCACCGCGACACGCACGGCTTCAACGA
AGCGCTGCGCTCGGCACTGCGCGAAGACCCGGACATCATCCTGGTCGGCGAATTGCGCGACCTGGAAACCATTCGCCTGG
CGCTCACCGCTGCGGAAACCGGTCACTTGGTGTTCGGCACCCTGCACACCAGCTCGGCCGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGCGCTGTGATTTCGCAGGC
GCTGCTGAAGAAGGTCGGCGGCGGCCGTACCGCAGCATGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCCTCGATCCAGACCGGCCAGCAATACGGCATGCAGACGCTGGACCAGCATCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACGCGCAACCAGGCTCGCGAGTACGCCAAGGACAAGCGGATATTCGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.942

99.71

0.797
  pilT Acinetobacter baumannii D1279779

79.942

99.71

0.797
  pilT Acinetobacter baumannii strain A118

79.942

99.71

0.797
  pilT Pseudomonas stutzeri DSM 10701

79.36

99.71

0.791
  pilT Acinetobacter baylyi ADP1

78.779

99.71

0.786
  pilT Pseudomonas aeruginosa PAK

79.056

98.261

0.777
  pilT Legionella pneumophila strain Lp02

75.291

99.71

0.751
  pilT Legionella pneumophila strain ERS1305867

75.291

99.71

0.751
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.125

95.362

0.678
  pilT Vibrio cholerae strain A1552

71.125

95.362

0.678
  pilT Neisseria meningitidis 8013

65.982

98.841

0.652
  pilT Neisseria gonorrhoeae MS11

65.689

98.841

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.499
  pilU Pseudomonas stutzeri DSM 10701

40.708

98.261

0.4
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397
  pilU Vibrio cholerae strain A1552

40.597

97.101

0.394

Multiple sequence alignment