Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilR   Type   Regulator
Locus tag   HW06_RS25330 Genome accession   NZ_CP008860
Coordinates   5369688..5371025 (+) Length   445 a.a.
NCBI ID   WP_017002067.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain H27930     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5364688..5376025
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HW06_RS25305 (HW06_00080) pgeF 5364831..5365559 (-) 729 WP_003102588.1 peptidoglycan editing factor PgeF -
  HW06_RS25310 (HW06_00085) rluD 5365556..5366518 (-) 963 WP_003094686.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  HW06_RS25315 (HW06_00090) - 5366664..5367689 (+) 1026 WP_023105576.1 outer membrane protein assembly factor BamD -
  HW06_RS25320 (HW06_00095) - 5367846..5368091 (+) 246 WP_003102591.1 PP0621 family protein -
  HW06_RS25325 (HW06_00100) pilS 5368081..5369673 (+) 1593 WP_059309305.1 two-component system sensor histidine kinase PilS Regulator
  HW06_RS25330 (HW06_00105) pilR 5369688..5371025 (+) 1338 WP_017002067.1 two-component system response regulator PilR Regulator
  HW06_RS25335 (HW06_00110) thiO 5371071..5372165 (-) 1095 WP_020750388.1 glycine oxidase ThiO -
  HW06_RS25340 (HW06_00115) - 5372300..5372809 (+) 510 WP_003102595.1 GspH/FimT family pseudopilin -
  HW06_RS25345 (HW06_00120) - 5372915..5373421 (+) 507 WP_003102598.1 GspH/FimT family pseudopilin -
  HW06_RS25350 (HW06_00125) pilV 5373412..5373969 (+) 558 WP_003119423.1 type 4a pilus minor pilin PilV Machinery gene
  HW06_RS25355 (HW06_00130) pilW 5373966..5374790 (+) 825 WP_003119422.1 type 4a pilus minor pilin PilW -
  HW06_RS25360 (HW06_00135) pilX 5374787..5375374 (+) 588 WP_003112826.1 type 4a pilus minor pilin PilX -

Sequence

Protein


Download         Length: 445 a.a.        Molecular weight: 49727.02 Da        Isoelectric Point: 5.8654

>NTDB_id=124940 HW06_RS25330 WP_017002067.1 5369688..5371025(+) (pilR) [Pseudomonas aeruginosa strain H27930]
MSRQKALIVDDEPDIRELLEITLGRMKLDTRSARNVKEARELLARESFDLCLTDMRLPDGSGLDLVQYIQQRHPQTPVAM
ITAYGSLDTAIQALKAGAFDFLTKPVDLGRLRELVATALRLRNPEAEEAPVDNRLLGESPPMRALRNQIGKLARSQAPVY
ISGESGSGKELVARLIHEQGPRIERPFVPVNCGAIPSELMESEFFGHKKGSFTGAIEDKQGLFQAASGGTLFLDEVADLP
MAMQVKLLRAIQEKAVRAVGGQQEVAVDVRILCATHKDLAAEVGAGRFRQDLYYRLNVIELRVPPLRERREDIPLLAERI
LKRLAGDTGLPAARLTGDAQEKLKNYRFPGNVRELENMLERAYTLCEDDQIQPHDLRLADAPGASQEGAASLSEIDNLED
YLEDIERKLIMQALEETRWNRTAAAQRLGLTFRSMRYRLKKLGID

Nucleotide


Download         Length: 1338 bp        

>NTDB_id=124940 HW06_RS25330 WP_017002067.1 5369688..5371025(+) (pilR) [Pseudomonas aeruginosa strain H27930]
ATGAGCCGACAAAAAGCCCTGATCGTCGACGATGAACCGGATATCCGCGAACTGCTGGAAATCACTCTCGGCCGCATGAA
GCTGGACACCCGCAGCGCCCGCAACGTCAAGGAAGCCCGCGAGTTGCTGGCCCGCGAGTCGTTCGACCTGTGCCTCACCG
ACATGCGCCTGCCGGACGGCAGCGGCCTCGATCTGGTCCAGTACATCCAGCAGCGCCATCCACAGACCCCGGTGGCCATG
ATCACCGCGTACGGCAGCCTGGACACCGCGATCCAGGCGCTCAAGGCCGGTGCCTTCGACTTCCTCACCAAACCGGTCGA
CCTCGGTCGCTTGCGGGAGCTGGTGGCAACCGCCCTGCGCTTGCGCAACCCGGAAGCCGAGGAAGCGCCGGTGGACAACC
GCCTGCTCGGCGAGTCGCCGCCGATGCGCGCCCTGCGCAACCAGATCGGCAAGCTGGCGCGCAGCCAGGCGCCGGTCTAC
ATCAGTGGCGAGTCCGGTAGCGGCAAGGAGCTGGTGGCGCGCCTGATCCACGAGCAGGGGCCACGTATCGAGCGGCCGTT
CGTGCCGGTGAACTGCGGCGCGATTCCCTCCGAACTGATGGAAAGCGAGTTCTTCGGCCACAAGAAAGGCAGCTTCACTG
GCGCTATCGAAGACAAGCAGGGCCTGTTCCAGGCCGCCAGCGGCGGCACCCTGTTCCTCGACGAAGTCGCCGACCTGCCG
ATGGCCATGCAGGTCAAACTGCTCCGGGCGATCCAGGAAAAGGCCGTGCGCGCGGTCGGCGGCCAGCAGGAGGTCGCCGT
CGACGTGCGCATCCTCTGCGCCACCCACAAGGACCTCGCCGCCGAAGTCGGCGCCGGGCGCTTCCGCCAGGACCTCTACT
ACCGCCTCAACGTCATCGAGCTGCGCGTACCGCCGCTGCGCGAACGCCGCGAGGACATCCCGCTGCTCGCCGAACGCATC
CTCAAGCGCCTGGCCGGCGACACCGGCCTGCCGGCCGCCAGGCTGACCGGCGACGCACAGGAGAAGCTGAAGAACTACCG
CTTCCCGGGCAACGTCCGCGAGCTGGAAAACATGCTGGAGCGCGCCTACACCCTGTGCGAAGACGACCAGATCCAGCCTC
ACGACCTGCGCCTGGCCGATGCGCCGGGTGCCAGCCAGGAAGGCGCCGCGAGCCTGAGCGAAATAGACAACCTCGAGGAC
TACCTGGAAGACATCGAGCGCAAGCTGATCATGCAGGCGCTCGAGGAGACCCGCTGGAACCGCACCGCCGCGGCCCAGCG
CCTGGGCCTGACGTTCCGCTCGATGCGCTACCGCCTGAAAAAGCTGGGCATCGACTGA

Domains

Predicted by InterproScan.

Sigma-54 interaction domain (Sigma54_activat)

(135-301)

Response regulator receiver domain (Response_reg)

(7-115)

Bacterial regulatory protein, Fis family (HTH_8)

(402-442)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilR Pseudomonas aeruginosa PAK

99.775

100

0.998
  pilR Acinetobacter baumannii strain A118

51.198

100

0.528
  luxO Vibrio cholerae strain A1552

38.17

100

0.384

Multiple sequence alignment