Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   HW08_RS25510 Genome accession   NZ_CP008858
Coordinates   5299194..5300411 (+) Length   405 a.a.
NCBI ID   WP_003116250.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain F63912     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5294194..5305411
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HW08_RS25485 (HW08_00210) nadC 5294944..5295792 (+) 849 WP_016562338.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HW08_RS25495 (HW08_00200) - 5295957..5296655 (-) 699 WP_023122772.1 hypothetical protein -
  HW08_RS25500 (HW08_00195) - 5296719..5297258 (-) 540 WP_003116248.1 pilin -
  HW08_RS25505 (HW08_00190) pilB 5297487..5299190 (+) 1704 WP_003116249.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HW08_RS25510 (HW08_00185) pilC 5299194..5300411 (+) 1218 WP_003116250.1 type II secretion system F family protein Machinery gene
  HW08_RS25515 (HW08_00180) pilD 5300414..5301286 (+) 873 WP_016562340.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  HW08_RS25520 (HW08_00175) coaE 5301283..5301894 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  HW08_RS25525 (HW08_00170) yacG 5301891..5302091 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  HW08_RS25530 (HW08_00165) - 5302128..5302337 (-) 210 WP_003094660.1 hypothetical protein -
  HW08_RS25535 (HW08_00160) - 5302443..5303132 (-) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  HW08_RS25540 (HW08_00155) - 5303129..5303599 (-) 471 WP_003094664.1 hypothetical protein -
  HW08_RS25545 (HW08_00150) - 5303596..5304021 (-) 426 WP_016562341.1 GNAT family N-acetyltransferase -
  HW08_RS25550 (HW08_00145) - 5304154..5304783 (+) 630 WP_003094668.1 DUF1780 domain-containing protein -
  HW08_RS25555 (HW08_00140) - 5304780..5305229 (+) 450 WP_003094670.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44051.94 Da        Isoelectric Point: 9.8417

>NTDB_id=124867 HW08_RS25510 WP_003116250.1 5299194..5300411(+) (pilC) [Pseudomonas aeruginosa strain F63912]
MADKALKTSVFIWEGTDKKGSKVKGELAGQNPMLVKAQLRKQGINPLKVRKKGITLFGAGKKIKPMDIALFTRQMSTMMG
AGVPLLQSFDIIGEGFENPNMRKLVDEIKQDVAAGNSLASSLRKKPIYFDDLYCNLVDAGEQSGALETLLDRVATYKEKT
ESLKAKIKKAMTYPIAVIVVALVVSAILLIKVVPQFQSVFANFGAELPAFTQMVINLSEILQEWWLIVLIGLFAAAFAFR
EVHRRSQKLRDSVDRGLLKLPIIGGILYKSAIARYARTLSTTFAAGVPLVEALDSVSGATGNVVFKNAVTKIKQDVSSGM
QLNFSMRTTNVFPSMAIQMAAIGEESGSLDEMLGKVAGFYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGSVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=124867 HW08_RS25510 WP_003116250.1 5299194..5300411(+) (pilC) [Pseudomonas aeruginosa strain F63912]
ATGGCGGACAAAGCGTTAAAGACCAGTGTCTTCATCTGGGAAGGCACTGATAAGAAAGGCAGCAAGGTCAAAGGAGAGTT
GGCCGGGCAAAACCCTATGCTGGTGAAGGCTCAACTGCGCAAACAGGGTATCAACCCACTAAAGGTCCGCAAGAAAGGTA
TCACCCTGTTTGGGGCAGGGAAGAAGATTAAGCCCATGGACATCGCCCTGTTCACTCGGCAGATGTCTACCATGATGGGT
GCCGGCGTACCGTTACTGCAATCTTTTGACATCATCGGCGAAGGATTCGAAAATCCAAACATGCGCAAGCTAGTCGATGA
GATCAAGCAGGATGTTGCCGCCGGTAACAGCTTAGCCAGTTCACTTCGAAAGAAACCCATTTACTTCGATGATCTCTACT
GCAACCTAGTCGATGCTGGCGAACAGTCCGGTGCTTTGGAGACATTATTGGATCGGGTAGCAACTTATAAAGAAAAGACA
GAATCCCTGAAAGCCAAAATTAAAAAGGCCATGACTTATCCCATTGCAGTAATTGTAGTGGCCCTTGTGGTGTCGGCGAT
CCTTCTGATAAAAGTGGTCCCACAGTTCCAGTCCGTATTTGCAAATTTTGGCGCCGAGTTGCCGGCCTTTACTCAAATGG
TCATCAATCTTTCCGAGATTCTTCAAGAGTGGTGGCTCATAGTGCTTATTGGTCTTTTTGCCGCAGCTTTTGCATTTAGG
GAAGTTCATCGGCGCTCGCAAAAACTGAGAGATTCAGTAGATCGGGGCCTGCTGAAACTACCTATCATCGGCGGGATACT
TTACAAATCAGCTATCGCCCGCTACGCCCGAACGCTATCCACTACCTTTGCGGCTGGAGTGCCTCTGGTAGAAGCTCTGG
ACTCCGTTTCCGGAGCAACTGGCAACGTGGTATTCAAGAATGCCGTTACCAAGATCAAACAAGACGTGTCCAGCGGCATG
CAATTGAACTTTTCCATGCGCACCACCAATGTTTTCCCCAGTATGGCTATCCAGATGGCTGCAATTGGCGAGGAATCAGG
TTCGCTAGATGAGATGTTAGGAAAAGTTGCCGGTTTCTATGAGGAAGAAGTCGATAACGCCGTCGACAACCTGACAACGT
TGATGGAGCCGATGATCATGGCCGTGCTCGGCGTACTGGTCGGCGGCCTGATCATCGCTATGTATCTACCGATCTTCCAA
CTGGGTTCCGTTGTGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

79.506

100

0.795
  pilC Acinetobacter baumannii D1279779

60.644

99.753

0.605
  pilC Acinetobacter baylyi ADP1

59.606

100

0.598
  pilC Legionella pneumophila strain ERS1305867

55.051

97.778

0.538
  pilG Neisseria meningitidis 44/76-A

46.269

99.259

0.459
  pilG Neisseria gonorrhoeae MS11

46.02

99.259

0.457
  pilC Vibrio cholerae strain A1552

42.222

100

0.422
  pilC Vibrio campbellii strain DS40M4

40.909

97.778

0.4
  pilC Thermus thermophilus HB27

38.404

99.012

0.38

Multiple sequence alignment