Detailed information NTG0124754

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: EP13_RS14025
• Genome accession: NZ_CP008849
• Coordinates: 3181076..3181957 (+)
• Length: 293 a.a.
• NCBI ID: WP_044057825.1
• Uniprot ID: A0A075P481
• Organism: Alteromonas australica strain H 17
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3176076..3186957

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EP13_RS14000 (EP13_14325)	ampD	3176849..3177391 (-)	543	WP_044057820.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
EP13_RS14005 (EP13_14330)	-	3177503..3178021 (+)	519	WP_044057821.1	TIGR02281 family clan AA aspartic protease	-
EP13_RS14010 (EP13_14335)	nadC	3178040..3178903 (+)	864	WP_044057822.1	carboxylating nicotinate-nucleotide diphosphorylase	-
EP13_RS14015 (EP13_14340)	-	3179219..3179650 (+)	432	WP_044057823.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
EP13_RS14020 (EP13_14345)	pilC	3179765..3180976 (+)	1212	WP_044057824.1	type II secretion system F family protein	Machinery gene
EP13_RS14025 (EP13_14350)	pilD	3181076..3181957 (+)	882	WP_044057825.1	A24 family peptidase	Machinery gene
EP13_RS14030 (EP13_14355)	coaE	3181957..3182565 (+)	609	WP_044057826.1	dephospho-CoA kinase	-
EP13_RS14035 (EP13_14360)	zapD	3182695..3183447 (+)	753	WP_044057827.1	cell division protein ZapD	-
EP13_RS14040 (EP13_14365)	yacG	3183484..3183711 (+)	228	WP_044057828.1	DNA gyrase inhibitor YacG	-

Sequence

Protein

• Length: 293 a.a.
• Molecular weight: 32581.79 Da
• Isoelectric Point: 7.6329

>NTDB_id=124754 EP13_RS14025 WP_044057825.1 3181076..3181957(+) (pilD) [Alteromonas australica strain H 17]
MEALISLSQLYPPFFYGLVFLVSLMVGSFLNVVIHRLPIMMENSWKHEYSAYFTNENIPQNDASGPFNLVKPDSTCPACG
HKIRPWENIPVISYLCLKGKCASCKTPISLRYPFVELFTALTCTFAAFYFGPSPQALWAMVLTYFLVAMLFIDLDKMLLP
DQLTLPLLWLGLLLSTHSVFVDMQSAIWGAAIGYVSLWLVYWMFKIATGKEGMGYGDFKLLAALGAFVGWQGLPIIILLS
SLVGAIAGVAIMMFQNKGKSVAIPFGPYLAVAGWITLLFKSHITQAYLDWILG

Nucleotide

• Length: 882 bp

>NTDB_id=124754 EP13_RS14025 WP_044057825.1 3181076..3181957(+) (pilD) [Alteromonas australica strain H 17]
ATGGAAGCGCTTATTTCACTAAGCCAGCTTTATCCCCCTTTCTTTTATGGCCTTGTTTTTCTCGTCAGTTTGATGGTGGG
AAGCTTTTTGAATGTGGTGATTCATCGCCTGCCTATCATGATGGAAAATAGCTGGAAGCACGAATATAGCGCTTATTTTA
CGAATGAAAACATCCCCCAAAACGACGCTTCTGGCCCCTTCAATTTAGTTAAGCCTGATAGCACCTGCCCTGCCTGCGGA
CATAAAATTCGCCCTTGGGAGAACATTCCCGTTATCAGTTATTTATGCTTGAAAGGAAAATGTGCATCGTGCAAAACCCC
TATTTCGTTGCGCTATCCCTTTGTTGAGTTATTCACTGCCCTCACCTGTACATTCGCGGCTTTTTATTTCGGCCCTTCTC
CTCAAGCGCTTTGGGCAATGGTATTGACTTACTTTTTGGTGGCCATGCTATTTATTGACCTAGATAAAATGCTGCTACCG
GATCAGCTCACACTGCCCTTGCTATGGTTAGGGTTACTCTTGAGTACACACTCTGTCTTTGTTGATATGCAATCAGCCAT
TTGGGGCGCAGCCATTGGCTACGTGAGTTTGTGGTTAGTGTATTGGATGTTTAAAATTGCCACTGGAAAAGAAGGTATGG
GCTATGGCGATTTTAAGCTACTGGCAGCGCTGGGCGCCTTTGTAGGCTGGCAAGGCTTACCTATCATTATTTTACTTTCT
TCTTTAGTGGGCGCCATTGCCGGTGTGGCAATTATGATGTTCCAAAACAAAGGTAAGTCGGTGGCGATTCCATTTGGCCC
TTACTTAGCCGTTGCAGGGTGGATAACTTTGCTGTTTAAATCACACATTACTCAAGCCTACTTAGATTGGATATTAGGTT
GA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (140-248)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (22-129)

3D structure

• AlphaFold DB: A0A075P481

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	51.399	97.611	0.502
pilD	Vibrio cholerae strain A1552	50.704	96.928	0.491
pilD	Acinetobacter baumannii D1279779	51.46	93.515	0.481
pilD	Acinetobacter nosocomialis M2	51.095	93.515	0.478
pilD	Neisseria gonorrhoeae MS11	48.936	96.246	0.471