Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   VAB027_RS17275 Genome accession   NZ_CP010812
Coordinates   3720038..3721765 (-) Length   575 a.a.
NCBI ID   WP_046127160.1    Uniprot ID   -
Organism   Vibrio cholerae strain 10432-62     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3715038..3726765
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VAB027_RS17240 (VAB027_3418) - 3715304..3716074 (-) 771 WP_046127156.1 PilW family protein -
  VAB027_RS17245 (VAB027_3419) - 3716074..3716640 (-) 567 WP_046127157.1 type II secretion system protein -
  VAB027_RS17250 (VAB027_3420) - 3716630..3717142 (-) 513 WP_046127381.1 type II secretion system protein -
  VAB027_RS17255 (VAB027_3421) - 3717203..3717682 (-) 480 WP_052724884.1 type II secretion system protein -
  VAB027_RS17260 (VAB027_3422) - 3717717..3718271 (-) 555 WP_000738708.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  VAB027_RS17265 (VAB027_3423) - 3718352..3718804 (-) 453 WP_046127159.1 hypothetical protein -
  VAB027_RS17270 (VAB027_3424) - 3718807..3720029 (-) 1223 Protein_3324 type II secretion system F family protein -
  VAB027_RS17275 (VAB027_3426) pilB/pilB1 3720038..3721765 (-) 1728 WP_046127160.1 GspE/PulE family protein Machinery gene
  VAB027_RS17280 (VAB027_3427) - 3721755..3722906 (-) 1152 WP_046127161.1 lipopolysaccharide assembly protein LapB -
  VAB027_RS17285 (VAB027_3428) - 3722903..3723748 (-) 846 WP_046127162.1 ExeA family protein -
  VAB027_RS17290 (VAB027_3429) mshL 3723753..3725417 (-) 1665 WP_001227128.1 pilus (MSHA type) biogenesis protein MshL -
  VAB027_RS17295 (VAB027_3430) - 3725432..3725749 (-) 318 WP_046127163.1 MSHA biogenesis protein MshK -
  VAB027_RS17300 (VAB027_3431) gspM 3725742..3726392 (-) 651 WP_000835144.1 type II secretion system protein GspM -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63871.36 Da        Isoelectric Point: 6.1676

>NTDB_id=124552 VAB027_RS17275 WP_046127160.1 3720038..3721765(-) (pilB/pilB1) [Vibrio cholerae strain 10432-62]
MPINKLRKRLGDLLVEEGIVSEAQLEQALNAQKNTGRRLGDTLISLGFLSETQLLNFLAQQLSLPVIDLSRAHVDIDAVP
LLPEVHARRLRALVIGRSGDTLRIAMSDPADLFAQEALLNQLPDYGFEFVIAPEKQLVDGFDRYYRRTKEIVSFAEQLHA
EHKTNDSFDFEITDSDSDEVTVVKLINSLFEDAIQVGASDIHIEPDANVLRLRQRIDGVLHETLLHEVNIASALVLRLKL
MANLDISEKRLPQDGRFNIRVKGQSVDIRMSTMPIQHGESVVMRLLNQSAGVRKLEESGIPPHLLLRLRHQLKRPHGMIL
VTGPTGSGKTTTLYGALNELNTSGKKIITAEDPVEYRISRINQVQVNPKINLDFSTLLRTFLRQDPDIILVGEMRDQETV
EIGLRAALTGHLVLSTLHTNDAVDSALRLIDMGAPGYLVASAVRAVVAQRLVRKVCPDCSGHDEVDEARRQWLVTRFPNQ
VAAKFTRGRGCQNCNLTGYRGRIGVFEMLELDQPMMDCLRANDAVAFSKAARSNTDYKPLLASAMELALQGIVSLDEVMS
LGEGDSSGLVQPIYL

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=124552 VAB027_RS17275 WP_046127160.1 3720038..3721765(-) (pilB/pilB1) [Vibrio cholerae strain 10432-62]
GTGCCAATTAATAAACTGCGTAAACGGCTTGGTGACTTGCTGGTTGAAGAGGGGATTGTCAGCGAAGCGCAGCTTGAACA
AGCGCTCAATGCCCAGAAAAATACCGGACGCCGCTTAGGGGATACCTTAATTTCGCTTGGCTTTTTAAGCGAAACCCAGT
TGCTGAACTTTTTGGCGCAGCAATTGAGTTTACCTGTGATCGATTTGAGCCGCGCTCATGTCGATATTGATGCGGTTCCT
TTACTGCCTGAAGTTCATGCCCGCCGTTTGCGTGCGCTGGTGATAGGCCGCAGTGGTGATACGCTGCGCATAGCCATGAG
TGATCCTGCGGATCTGTTTGCTCAAGAAGCCTTGCTCAACCAGTTGCCTGATTACGGTTTTGAGTTTGTCATCGCTCCTG
AAAAACAGTTGGTCGATGGCTTCGATCGTTACTATCGCCGCACCAAAGAAATTGTTTCGTTCGCCGAACAATTGCATGCC
GAACACAAAACCAATGATAGTTTTGATTTTGAGATCACCGATTCAGATAGCGATGAAGTGACGGTTGTTAAACTGATCAA
CTCGCTGTTTGAAGATGCGATTCAAGTAGGAGCCTCGGATATACACATTGAGCCGGATGCCAACGTACTGCGTTTGCGGC
AACGGATCGATGGGGTATTGCATGAAACCTTACTTCATGAAGTGAATATCGCCTCTGCCTTAGTGCTGCGCTTAAAATTA
ATGGCGAATCTGGATATTTCAGAAAAACGCCTTCCGCAAGATGGCCGCTTTAACATCCGTGTAAAAGGACAGTCCGTCGA
TATCCGGATGTCAACCATGCCTATCCAGCACGGTGAATCAGTGGTGATGCGTTTGCTCAACCAATCGGCGGGGGTTAGAA
AGCTCGAAGAGTCCGGTATTCCGCCTCATTTATTGCTGCGTTTGCGCCATCAGTTGAAACGCCCTCATGGCATGATTCTG
GTGACTGGGCCTACCGGCTCGGGGAAAACCACCACCTTGTATGGCGCACTCAACGAGCTCAATACGTCCGGCAAAAAAAT
CATTACTGCGGAAGATCCGGTGGAATACCGGATTTCACGGATCAATCAGGTGCAAGTGAACCCGAAAATCAATCTCGATT
TCTCGACGTTACTGCGCACTTTCTTGCGCCAAGACCCAGATATTATTTTGGTCGGTGAGATGCGTGACCAAGAGACGGTC
GAAATTGGCCTGCGCGCCGCATTAACCGGTCACTTAGTCTTAAGTACTCTGCACACCAATGATGCCGTCGATAGCGCGCT
GCGTTTGATTGATATGGGGGCGCCCGGTTATCTGGTTGCCAGTGCGGTACGTGCCGTGGTTGCGCAGCGTTTAGTACGAA
AAGTCTGTCCGGATTGTAGTGGACATGATGAGGTGGATGAAGCGCGTCGCCAATGGCTTGTGACGCGTTTCCCTAATCAA
GTTGCCGCCAAATTCACTCGTGGACGCGGCTGTCAAAACTGTAACTTAACTGGTTATCGCGGCCGGATCGGTGTATTTGA
AATGCTTGAGCTGGATCAGCCGATGATGGATTGCTTGCGGGCAAATGATGCAGTGGCCTTCTCTAAAGCCGCACGCAGTA
ACACAGATTATAAACCGCTACTGGCTTCGGCGATGGAGTTAGCGCTACAAGGCATCGTCAGTCTTGATGAAGTGATGTCG
CTGGGTGAAGGTGATTCCTCTGGTTTGGTTCAACCGATTTATCTGTAG

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-149)

Type II/IV secretion system protein (T2SSE)

(189-454)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

35.596

100

0.374
  pilB Vibrio campbellii strain DS40M4

36.796

98.783

0.363
  pilB Vibrio cholerae strain A1552

36.945

97.913

0.362
  pilF Thermus thermophilus HB27

37.209

97.217

0.362
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.477

96.522

0.362
  pilB Legionella pneumophila strain ERS1305867

39.057

92.174

0.36