Detailed information NTG0124126

Bioinformatically predicted

Overview

• Name: amiE
• Type: Regulator
• Locus tag: SP5448_RS01350
• Genome accession: NZ_CP008776
• Coordinates: 255911..256981 (+)
• Length: 356 a.a.
• NCBI ID: WP_010921893.1
• Uniprot ID: A0A4U9C068
• Organism: Streptococcus pyogenes strain 5448
• Function: internalize XIP (predicted from homology) Competence regulation

Genomic Context

Location: 250911..261981

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SP5448_RS01335 (SP5448_01400)	amiA	251439..253409 (+)	1971	WP_011285389.1	peptide ABC transporter substrate-binding protein	Regulator
SP5448_RS01340 (SP5448_01405)	amiC	253474..254976 (+)	1503	WP_010921891.1	ABC transporter permease	Regulator
SP5448_RS01345 (SP5448_01410)	amiD	254976..255902 (+)	927	WP_010921892.1	oligopeptide ABC transporter permease OppC	Regulator
SP5448_RS01350 (SP5448_01415)	amiE	255911..256981 (+)	1071	WP_010921893.1	ABC transporter ATP-binding protein	Regulator
SP5448_RS01355 (SP5448_01420)	amiF	256974..257897 (+)	924	WP_002986000.1	ABC transporter ATP-binding protein	Regulator
SP5448_RS09980	-	257935..258024 (-)	90	WP_077707078.1	IS3 family transposase	-

Sequence

Protein

• Length: 356 a.a.
• Molecular weight: 39703.77 Da
• Isoelectric Point: 5.6707

>NTDB_id=124126 SP5448_RS01350 WP_010921893.1 255911..256981(+) (amiE) [Streptococcus pyogenes strain 5448]
MTKENNVILTAKDVVVEFDVRDRVLTAIRNVSLELVEGEVLAFVGESGSGKSVLTKTFTGMLESNGRIANGSIVYRGQEL
TDLKTNKEWAKIRGSKIATIFQDPMTSLSPIKTIGSQITEVIIKHQKVSHAKAKEMALDYMNKVGIPNAKKRFEDYPFEY
SGGMRQRIVIAIALACRPDILICDEPTTALDVTIQAQIVELLKSLQREYHFTIIFITHDLGVVASIADKVAVMYAGEIVE
FGTVEEIFYDPRHPYTWSLLSSLPQLADESGELYAIPGTPPSLYSPIIGDAFALRSEYAMVLDFEKAPPAINVSETHWAK
TWLLHPEAPKVQKPEVIQDLHQKILRKMSQQEEGNV

Nucleotide

• Length: 1071 bp

>NTDB_id=124126 SP5448_RS01350 WP_010921893.1 255911..256981(+) (amiE) [Streptococcus pyogenes strain 5448]
ATGACAAAAGAAAATAATGTAATCTTAACTGCCAAAGATGTGGTGGTAGAATTTGATGTGCGTGATCGTGTTTTAACAGC
TATCCGTAACGTCTCACTGGAACTTGTTGAAGGAGAAGTCCTTGCTTTTGTAGGGGAATCAGGCTCAGGTAAATCTGTTT
TAACAAAGACCTTTACAGGGATGTTGGAGTCTAATGGACGCATTGCTAATGGTTCAATTGTCTATCGTGGGCAAGAATTG
ACAGATTTAAAAACAAATAAAGAGTGGGCAAAGATTCGCGGCTCAAAAATCGCAACGATTTTCCAAGACCCAATGACCAG
TCTTAGTCCCATTAAAACTATCGGTAGCCAAATCACAGAAGTGATTATTAAGCACCAAAAAGTAAGTCATGCCAAAGCTA
AAGAAATGGCCCTTGATTACATGAATAAAGTGGGTATCCCAAATGCCAAAAAACGCTTTGAAGATTACCCATTTGAGTAT
TCAGGAGGAATGCGTCAACGTATTGTTATCGCTATTGCTTTAGCTTGTCGCCCAGATATTCTTATCTGTGATGAGCCAAC
AACAGCCCTTGATGTGACTATTCAAGCTCAAATCGTTGAGTTATTGAAGTCGCTTCAACGAGAATATCATTTCACCATTA
TCTTTATTACGCACGATTTAGGTGTTGTGGCAAGCATTGCAGATAAAGTGGCTGTCATGTATGCAGGAGAAATTGTTGAA
TTTGGAACAGTCGAAGAGATTTTCTATGATCCAAGACACCCCTATACATGGAGTTTGCTGTCTAGCTTACCGCAGTTGGC
AGATGAATCTGGTGAACTTTACGCTATTCCAGGAACGCCTCCATCACTTTATTCACCAATTATCGGAGATGCCTTTGCAC
TTCGCTCAGAATATGCTATGGTTTTAGACTTTGAAAAAGCACCTCCGGCGATTAACGTATCTGAGACTCATTGGGCCAAA
ACATGGCTTTTACACCCAGAGGCTCCAAAAGTTCAAAAACCAGAAGTCATTCAAGATTTGCATCAAAAAATCTTAAGAAA
AATGTCACAACAGGAGGAAGGAAATGTCTGA

Domains

Predicted by InterproScan.

• ABC transporter (ABC_tran) (29-187)

• Oligopeptide/dipeptide transporter, C-terminal region (oligo_HPY) (239-299)

3D structure

• AlphaFold DB: A0A4U9C068

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
amiE	Streptococcus salivarius strain HSISS4	76.667	100	0.775
amiE	Streptococcus thermophilus LMG 18311	75.556	100	0.764
amiE	Streptococcus thermophilus LMD-9	75.556	100	0.764
oppD	Streptococcus mutans UA159	56.677	94.663	0.537