Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYF   Type   Machinery gene
Locus tag   DK93_RS00675 Genome accession   NZ_CP007562
Coordinates   105026..105460 (+) Length   144 a.a.
NCBI ID   WP_002992738.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain NGAS327     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 100026..110460
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DK93_RS00645 (DK93_00660) - 101694..102059 (+) 366 WP_002986560.1 DUF1033 family protein -
  DK93_RS00650 (DK93_00665) comYA 102152..103090 (+) 939 WP_002993471.1 competence type IV pilus ATPase ComGA Machinery gene
  DK93_RS00655 (DK93_00670) comGB 103026..104061 (+) 1036 Protein_83 competence type IV pilus assembly protein ComGB -
  DK93_RS00660 (DK93_00675) comYC 104063..104389 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  DK93_RS00665 (DK93_00680) comGD 104364..104792 (+) 429 WP_023605232.1 competence type IV pilus minor pilin ComGD -
  DK93_RS00670 (DK93_00685) comGE 104749..105033 (+) 285 WP_011284422.1 competence type IV pilus minor pilin ComGE -
  DK93_RS00675 (DK93_00690) comYF 105026..105460 (+) 435 WP_002992738.1 competence type IV pilus minor pilin ComGF Machinery gene
  DK93_RS00680 (DK93_00695) comGG 105444..105770 (+) 327 WP_002992739.1 competence type IV pilus minor pilin ComGG -
  DK93_RS00685 (DK93_00700) comYH 105868..106821 (+) 954 WP_023610092.1 class I SAM-dependent methyltransferase Machinery gene
  DK93_RS00690 (DK93_00705) - 106880..108076 (+) 1197 WP_038431193.1 acetate kinase -
  DK93_RS00695 (DK93_00710) - 108263..108571 (+) 309 WP_032467031.1 hypothetical protein -
  DK93_RS00700 (DK93_00715) proC 108654..109424 (-) 771 WP_002992745.1 pyrroline-5-carboxylate reductase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16645.27 Da        Isoelectric Point: 10.2526

>NTDB_id=120922 DK93_RS00675 WP_002992738.1 105026..105460(+) (comYF) [Streptococcus pyogenes strain NGAS327]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIEQSQICITLKWKSGLERTFYYAFQD

Nucleotide


Download         Length: 435 bp        

>NTDB_id=120922 DK93_RS00675 WP_002992738.1 105026..105460(+) (comYF) [Streptococcus pyogenes strain NGAS327]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACCCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAAACCCTCCTTAAACGTAGCCATTATCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGATTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAA
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTTCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGAGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(41-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

51.079

96.528

0.493
  comYF Streptococcus mutans UA159

50.36

96.528

0.486
  comGF Lactococcus lactis subsp. cremoris KW2

46.715

95.139

0.444
  comGF/cglF Streptococcus mitis NCTC 12261

44.776

93.056

0.417
  comGF/cglF Streptococcus pneumoniae Rx1

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae D39

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae R6

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae TIGR4

43.609

92.361

0.403
  comGF/cglF Streptococcus mitis SK321

42.857

92.361

0.396

Multiple sequence alignment