Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   D082_RS15120 Genome accession   NZ_CP007542
Coordinates   3320360..3321202 (-) Length   280 a.a.
NCBI ID   WP_028948289.1    Uniprot ID   A0A068N172
Organism   Synechocystis sp. PCC 6714     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3315360..3326202
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D082_RS15105 (D082_33390) - 3316303..3317055 (+) 753 WP_028948292.1 FkbM family methyltransferase -
  D082_RS15110 (D082_33400) - 3317073..3317897 (+) 825 WP_028948291.1 FkbM family methyltransferase -
  D082_RS15115 (D082_33410) pilQ 3317906..3320263 (-) 2358 WP_238546753.1 type IV pilus secretin PilQ Machinery gene
  D082_RS15120 (D082_33420) pilO 3320360..3321202 (-) 843 WP_028948289.1 hypothetical protein Machinery gene
  D082_RS15125 (D082_33430) pilN 3321254..3322102 (-) 849 WP_028948288.1 PilN domain-containing protein Machinery gene
  D082_RS15130 (D082_33440) pilM 3322217..3323320 (-) 1104 WP_028948287.1 type IV pilus biogenesis protein PilM Machinery gene
  D082_RS15135 (D082_33460) - 3323519..3323959 (-) 441 WP_028948286.1 hypothetical protein -
  D082_RS15140 (D082_33470) - 3323988..3324752 (-) 765 WP_028948285.1 iron uptake porin -
  D082_RS15145 (D082_33480) - 3325018..3325758 (-) 741 WP_028948284.1 WecB/TagA/CpsF family glycosyltransferase -

Sequence

Protein


Download         Length: 280 a.a.        Molecular weight: 30599.57 Da        Isoelectric Point: 4.0147

>NTDB_id=120746 D082_RS15120 WP_028948289.1 3320360..3321202(-) (pilO) [Synechocystis sp. PCC 6714]
MTSYSEEYSSTTTIIDEEFDAGGNYPTAFGITFTPQVSGISLGILGLLAMVYVVLNFLLPALGEYQQLKQDEQGKQAQVD
QQSPEILMRRLANAEVQLQEAQQRKATVLELYANSEDLNTILYNFNTLFVNRNMKLLSFLPQGEPAVISDDSLGANVNNQ
LKRQTYTISMAGSFRDTLGVIRDIERLQPLILMKNLRTSLVNAEFPVNVVRDGNTVQVRTDAPDTLTTDLTLEVLMPLTP
EEIAALTPPPPPPGQEGQPAEGQPPAEGQPPAEGEAPPAP

Nucleotide


Download         Length: 843 bp        

>NTDB_id=120746 D082_RS15120 WP_028948289.1 3320360..3321202(-) (pilO) [Synechocystis sp. PCC 6714]
ATGACTAGCTACAGCGAAGAGTATTCGTCAACCACAACGATTATTGATGAGGAATTTGATGCGGGGGGCAATTATCCAAC
GGCATTTGGTATCACATTCACTCCCCAGGTGAGCGGCATCAGCCTAGGAATTCTTGGTTTATTGGCCATGGTCTATGTTG
TGCTCAATTTTTTGCTTCCGGCTTTAGGGGAGTATCAGCAACTCAAACAGGACGAACAGGGTAAGCAGGCCCAGGTAGAC
CAACAATCCCCAGAAATCCTGATGCGCCGGTTGGCCAATGCAGAGGTCCAGTTGCAGGAAGCACAGCAAAGGAAGGCAAC
GGTGCTAGAACTCTATGCCAATAGTGAGGATTTGAATACAATCCTATATAACTTCAATACTTTGTTTGTTAACAGAAACA
TGAAGTTATTAAGTTTTTTGCCCCAGGGGGAACCAGCGGTGATCTCCGATGATAGTTTGGGAGCCAATGTCAATAACCAG
CTTAAACGCCAAACCTACACCATTTCTATGGCCGGTAGCTTTAGGGATACTTTGGGGGTAATTCGGGACATTGAAAGGCT
ACAACCATTGATTTTGATGAAAAATTTACGAACTAGCCTGGTCAATGCGGAGTTTCCCGTCAATGTGGTGAGGGATGGAA
ACACGGTGCAGGTACGAACTGATGCTCCGGATACTCTGACCACAGACCTGACGTTGGAGGTCCTTATGCCCTTGACTCCC
GAGGAAATCGCGGCCTTGACTCCCCCGCCCCCGCCCCCTGGTCAGGAGGGACAACCGGCGGAAGGACAGCCTCCCGCAGA
AGGACAGCCCCCTGCGGAAGGGGAAGCGCCTCCGGCCCCTTAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A068N172

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Synechocystis sp. PCC 6803

87.5

100

0.875

Multiple sequence alignment