Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   D082_RS15130 Genome accession   NZ_CP007542
Coordinates   3322217..3323320 (-) Length   367 a.a.
NCBI ID   WP_028948287.1    Uniprot ID   A0A068N722
Organism   Synechocystis sp. PCC 6714     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3317217..3328320
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  D082_RS15115 (D082_33410) pilQ 3317906..3320263 (-) 2358 WP_238546753.1 type IV pilus secretin PilQ Machinery gene
  D082_RS15120 (D082_33420) pilO 3320360..3321202 (-) 843 WP_028948289.1 hypothetical protein Machinery gene
  D082_RS15125 (D082_33430) pilN 3321254..3322102 (-) 849 WP_028948288.1 PilN domain-containing protein Machinery gene
  D082_RS15130 (D082_33440) pilM 3322217..3323320 (-) 1104 WP_028948287.1 type IV pilus biogenesis protein PilM Machinery gene
  D082_RS15135 (D082_33460) - 3323519..3323959 (-) 441 WP_028948286.1 hypothetical protein -
  D082_RS15140 (D082_33470) - 3323988..3324752 (-) 765 WP_028948285.1 iron uptake porin -
  D082_RS15145 (D082_33480) - 3325018..3325758 (-) 741 WP_028948284.1 WecB/TagA/CpsF family glycosyltransferase -
  D082_RS15150 (D082_33490) - 3325953..3327539 (-) 1587 WP_028948283.1 TolC family protein -

Sequence

Protein


Download         Length: 367 a.a.        Molecular weight: 40872.64 Da        Isoelectric Point: 4.2651

>NTDB_id=120748 D082_RS15130 WP_028948287.1 3322217..3323320(-) (pilM) [Synechocystis sp. PCC 6714]
MFSRLTSLFGRKSSGVGLEITPERVNVAQLSAQGQSYKLRKFGHASIPEGIFEEGKIVDSPALAEIIQELLSEQKINSKQ
VATAVPMREAIIRMLPVPAELDENELRDMVLNHEAALYLPYPREEVDLDYQKLGLFEDEDGIEKVQVLLVATRREVTDSY
LDTFQQAGLQVDVLEINSFALIRTIREQLRQFGSREAAVLVDIEFDSTEIAIIVDGVPQFSRTVPIGTYQMQNALSRAMN
LPPSRNPEILQGMTIPVTQFDTMSTQGTAVNPGMNALMRVMGELTDELRRSINFYLNQSEELELVQLLLAGPGGGLIQLD
EYFTQRLSIPTVKVDPFEALAIDTDQNFSDIERPSLATVLGLGIREA

Nucleotide


Download         Length: 1104 bp        

>NTDB_id=120748 D082_RS15130 WP_028948287.1 3322217..3323320(-) (pilM) [Synechocystis sp. PCC 6714]
ATGTTTAGCCGTCTCACTAGCTTATTTGGTCGTAAATCCTCCGGGGTGGGGCTAGAAATCACCCCCGAAAGGGTCAATGT
GGCCCAGCTTTCGGCCCAGGGTCAGAGTTATAAACTGAGAAAATTTGGCCATGCTTCCATTCCAGAGGGTATTTTTGAGG
AAGGAAAGATCGTCGATTCCCCTGCCCTGGCGGAAATTATTCAGGAACTGTTGAGCGAACAAAAAATTAACAGTAAGCAA
GTGGCCACGGCGGTGCCCATGCGGGAAGCCATAATTCGTATGCTACCAGTGCCGGCGGAGTTGGACGAAAACGAGTTGCG
GGATATGGTGCTAAACCATGAGGCGGCCCTCTATTTGCCCTATCCCAGGGAAGAGGTGGATTTGGATTATCAAAAACTGG
GTTTATTTGAAGATGAAGACGGCATTGAAAAGGTACAGGTGTTGTTAGTGGCCACCCGTCGGGAGGTGACCGATTCCTAC
CTAGACACCTTTCAGCAGGCGGGGCTCCAGGTCGATGTACTAGAAATTAATAGTTTTGCCCTGATTAGGACTATTCGGGA
ACAACTCCGGCAATTTGGCTCCCGGGAAGCGGCGGTGCTGGTGGATATTGAGTTTGACAGCACGGAAATTGCCATTATCG
TCGATGGGGTGCCCCAGTTTTCCCGCACAGTGCCCATTGGCACTTATCAAATGCAGAATGCCCTTTCCCGGGCCATGAAT
TTACCCCCATCCCGGAATCCGGAAATTTTGCAGGGTATGACCATTCCTGTCACCCAGTTCGACACCATGAGCACCCAGGG
CACTGCAGTTAACCCCGGCATGAACGCCCTAATGCGGGTGATGGGGGAATTGACCGATGAGCTACGGCGATCGATTAACT
TTTACCTTAACCAGAGTGAGGAGTTGGAACTGGTGCAATTACTCCTGGCCGGTCCCGGTGGTGGCCTGATCCAATTGGAT
GAATATTTCACCCAACGCCTAAGCATTCCCACCGTTAAAGTTGATCCCTTTGAAGCCTTGGCGATCGATACGGACCAGAA
CTTTTCCGACATAGAGCGCCCTAGCCTAGCAACGGTTCTAGGTTTGGGCATACGGGAAGCCTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(17-241)

Type IV pilus assembly protein PilM; (PilM_2)

(282-365)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A068N722

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Synechocystis sp. PCC 6803

98.093

100

0.981

Multiple sequence alignment