Detailed information    

experimental Experimentally validated

Overview

Name   pilO   Type   Machinery gene
Locus tag   SGL_RS10695 Genome accession   NC_000911
Coordinates   1867737..1868564 (+) Length   275 a.a.
NCBI ID   WP_010872899.1    Uniprot ID   -
Organism   Synechocystis sp. PCC 6803     
Function   type IV pilus biogenesis and function   
DNA binding and uptake

Function

Competency of the natural transformation was lost in the slr0063, slr1274, slr1275, slr1276, slr1277 and sll1694 mutants and slr0197-disruptant. It was suggested that both DNA uptake for natural transformation and motility are mediated by a specific type IV-like pilus structure, while a putative DNA-binding protein encoded by slr0197 is additionally required for the DNA uptake.


Genomic Context

Location: 1862737..1873564
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SGL_RS10670 (slr1271) - 1863247..1863987 (+) 741 WP_010872894.1 WecB/TagA/CpsF family glycosyltransferase -
  SGL_RS20030 - 1864239..1865003 (+) 765 WP_010872895.1 iron uptake porin -
  SGL_RS10680 (slr1273) - 1865033..1865473 (+) 441 WP_010872896.1 hypothetical protein -
  SGL_RS10685 (slr1274) pilM 1865658..1866761 (+) 1104 WP_010872897.1 type IV pilus biogenesis protein PilM Machinery gene
  SGL_RS10690 (slr1275) pilN 1866843..1867697 (+) 855 WP_010872898.1 PilN domain-containing protein Machinery gene
  SGL_RS10695 (slr1276) pilO 1867737..1868564 (+) 828 WP_010872899.1 pilus assembly protein Machinery gene
  SGL_RS10700 (slr1277) pilQ 1868660..1871002 (+) 2343 WP_020862340.1 type IV pilus secretin PilQ Machinery gene
  SGL_RS10705 (sll1174) - 1871011..1871835 (-) 825 WP_010872901.1 FkbM family methyltransferase -
  SGL_RS10710 (sll1173) - 1871855..1872589 (-) 735 WP_010872902.1 FkbM family methyltransferase -

Sequence

Protein


Download         Length: 275 a.a.        Molecular weight: 30066.97 Da        Isoelectric Point: 3.9599

>NTDB_id=1413 SGL_RS10695 WP_010872899.1 1867737..1868564(+) (pilO) [Synechocystis sp. PCC 6803]
MTSYSEEYSSTTTIIDEEFDAGGNYPTAFGITFTPQVSGISLGILGLLAMGYVLLNFVMPALGEYQQLRQDEEAKQAQVD
QQSPEVLMRRLANAEVQLQEAQQRKATVLELYANSEDLNTILYNFNTLFVNRNVKLVSFLPQGDPVVISDDSLGANAKNQ
LKRQTYTISMTGGFSETLALIRDIERLQPLILMKNLRTSLVNPEFPVNVVRDGDTVQVQTDAPDILTTDLTLEVFMPLTP
EEIAALTPPPPPPGQEGQPPAEGQPPAEGQAAPAP

Nucleotide


Download         Length: 828 bp        

>NTDB_id=1413 SGL_RS10695 WP_010872899.1 1867737..1868564(+) (pilO) [Synechocystis sp. PCC 6803]
ATGACCAGCTACAGCGAAGAGTATTCATCAACGACAACAATTATTGATGAGGAATTTGATGCGGGAGGAAATTATCCAAC
TGCATTTGGGATAACCTTCACCCCCCAGGTAAGTGGCATTAGTTTAGGTATTCTTGGCTTGCTGGCCATGGGTTATGTTC
TGCTCAATTTTGTCATGCCAGCTTTGGGAGAATATCAACAACTAAGACAGGACGAAGAAGCTAAACAAGCTCAAGTGGAT
CAGCAGTCCCCGGAAGTGTTGATGCGTCGGCTAGCCAATGCGGAGGTGCAGTTACAGGAAGCTCAACAGCGTAAAGCGAC
AGTGTTGGAACTCTACGCCAATAGCGAGGACTTGAACACAATCCTCTATAACTTCAATACTCTATTCGTAAATAGAAACG
TGAAGTTAGTCAGCTTTTTGCCCCAGGGAGATCCGGTGGTAATCTCCGATGATAGTCTGGGGGCAAACGCAAAAAATCAG
CTTAAACGACAAACTTATACCATTTCCATGACGGGCGGTTTTAGTGAAACCCTGGCATTAATTCGGGATATTGAACGGCT
GCAACCGTTAATTTTGATGAAAAATTTGCGGACTAGCTTGGTTAATCCAGAATTTCCAGTCAATGTGGTTAGGGATGGTG
ACACTGTACAAGTGCAAACTGATGCGCCAGATATCCTAACTACGGATTTAACGTTGGAGGTGTTTATGCCATTAACCCCT
GAGGAGATTGCCGCCCTGACTCCTCCCCCGCCGCCGCCTGGACAGGAGGGTCAACCACCGGCTGAGGGACAACCTCCAGC
GGAAGGACAAGCGGCCCCGGCCCCCTAA

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value

References

[1] S Yoshihara et al. (2001) Mutational analysis of genes involved in pilus structure, motility and transformation competency in the unicellular motile cyanobacterium Synechocystis sp. PCC 6803. Plant & Cell Physiology 42(1):63-73. [PMID: 11158445]