Detailed information NTG0001414

Experimentally validated

Overview

• Name: pilQ
• Type: Machinery gene
• Locus tag: SGL_RS10700
• Genome accession: NC_000911
• Coordinates: 1868660..1871002 (+)
• Length: 780 a.a.
• NCBI ID: WP_020862340.1
• Uniprot ID: -
• Organism: Synechocystis sp. PCC 6803
• Function: type IV pilus biogenesis and function DNA binding and uptake

Function

Competency of the natural transformation was lost in the slr0063, slr1274, slr1275, slr1276, slr1277 and sll1694 mutants and slr0197-disruptant. It was suggested that both DNA uptake for natural transformation and motility are mediated by a specific type IV-like pilus structure, while a putative DNA-binding protein encoded by slr0197 is additionally required for the DNA uptake.

Genomic Context

Location: 1863660..1876002

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SGL_RS20030	-	1864239..1865003 (+)	765	WP_010872895.1	iron uptake porin	-
SGL_RS10680 (slr1273)	-	1865033..1865473 (+)	441	WP_010872896.1	hypothetical protein	-
SGL_RS10685 (slr1274)	pilM	1865658..1866761 (+)	1104	WP_010872897.1	type IV pilus biogenesis protein PilM	Machinery gene
SGL_RS10690 (slr1275)	pilN	1866843..1867697 (+)	855	WP_010872898.1	PilN domain-containing protein	Machinery gene
SGL_RS10695 (slr1276)	pilO	1867737..1868564 (+)	828	WP_010872899.1	pilus assembly protein	Machinery gene
SGL_RS10700 (slr1277)	pilQ	1868660..1871002 (+)	2343	WP_020862340.1	type IV pilus secretin PilQ	Machinery gene
SGL_RS10705 (sll1174)	-	1871011..1871835 (-)	825	WP_010872901.1	FkbM family methyltransferase	-
SGL_RS10710 (sll1173)	-	1871855..1872589 (-)	735	WP_010872902.1	FkbM family methyltransferase	-
SGL_RS10715 (slr1278)	tilS	1872656..1873648 (+)	993	WP_010872903.1	tRNA lysidine(34) synthetase TilS	-
SGL_RS10720 (sll1172)	thrC	1873698..1874846 (-)	1149	WP_010872904.1	threonine synthase	-
SGL_RS10725 (slr1279)	ndhC	1875129..1875491 (+)	363	WP_010872905.1	photosynthetic/respiratory NAD(P)H-quinone oxidoreductase subunit C	-

Sequence

Protein

• Length: 780 a.a.
• Molecular weight: 84098.34 Da
• Isoelectric Point: 4.3538

>NTDB_id=1414 SGL_RS10700 WP_020862340.1 1868660..1871002(+) (pilQ) [Synechocystis sp. PCC 6803]
MKNFRFWLTTEIATCCLLALAPAQAETVSQSNTLDGDLRTAIAGDSSRDWLQFEKSLEQSLKQKEEIDSWKPSLELMQAK
SLVKPGQKLTNIELLVQELEALSDPLALNFPEPNQTSVAQMAPPSRPMPPPPAGSGQVMFPNPEIIIQQQGGVPQRGASP
QVGNPSILSPAVPVAPVRSRAVPPPVGDLAISNINASFDMIDLGQRGQVNVPSLVLREAPAREVLAVLTRYAGMNLIFTD
NQNNEGTPTPGTPPGGQVAPPQAQSTITLDIQNESVQDVFNYVLMASGLKASRRGNTIFAGANLLPSARNIITRTIRLNQ
ASAESVASTLASQGAEVNILFEGQEDVQLAENAPPRVIKQPPTLVPLTVQKPANDSSVLILEGLVVSTDPRLNTVTLVGE
PRNVELASSMITQMDARRRQVAVNVKIIDINLNNIQDYDSSFSFGIGDSFFVQDSGSAVMRFGDTAPVQEIDINNNLGRI
TNPPAIVNPFQDGEIFFDLNRITNIEVPLGPGTIPINFFTSGSGAVSNNPLFNGVTEFPIVEVDEQGLLTITQPEFGLPS
FYQYPKKFQAQIDAQIRSGNAKILTDPTLIVQEGEAAQVKLTESVIASVDTQVDTQGDTAVRTITPVLEDVGLTLNVIVD
RIDDNGFITLRVNPIVASPAGTQVFDSGAGAINEITLINKRELTSGVVRLRDDQTFILSGIISELQRSTTSKVPILGDLP
VIGALFRQSTDTTDRSEVIILMTPKIIHDSTEAQFGFRYNPDAATAEFLRQKGFPVQAQP

Nucleotide

• Length: 2343 bp

>NTDB_id=1414 SGL_RS10700 WP_020862340.1 1868660..1871002(+) (pilQ) [Synechocystis sp. PCC 6803]
GTGAAAAATTTTCGGTTTTGGTTAACAACGGAAATAGCGACCTGTTGCTTGTTGGCCCTAGCGCCTGCCCAGGCAGAAAC
GGTTTCCCAGTCAAATACTTTGGATGGAGATTTGCGGACGGCGATCGCCGGTGACTCAAGTCGAGATTGGCTACAGTTTG
AAAAGAGTTTGGAGCAGTCTCTCAAGCAAAAAGAAGAAATTGATTCTTGGAAACCATCATTGGAGTTGATGCAGGCTAAA
TCCCTTGTCAAACCAGGGCAAAAGTTGACGAATATTGAGTTGCTGGTGCAGGAGTTGGAAGCCTTATCCGACCCACTGGC
ATTAAATTTTCCAGAACCGAACCAGACCAGTGTTGCTCAAATGGCTCCCCCCAGCCGCCCTATGCCTCCCCCTCCAGCGG
GCAGTGGGCAGGTCATGTTTCCCAATCCCGAAATAATCATCCAACAGCAGGGTGGTGTGCCCCAACGTGGTGCCAGCCCT
CAGGTGGGAAATCCTAGCATTTTATCTCCTGCGGTACCTGTGGCTCCGGTACGTTCCCGGGCTGTGCCGCCACCAGTGGG
GGATTTGGCCATTTCCAACATCAATGCTTCCTTCGACATGATTGATCTTGGCCAAAGGGGTCAGGTTAACGTACCAAGCT
TGGTACTACGGGAGGCCCCAGCAAGGGAAGTTTTGGCTGTGCTAACCCGTTATGCTGGCATGAATCTAATTTTTACCGAT
AACCAAAACAATGAAGGCACTCCTACCCCAGGTACACCTCCCGGTGGTCAGGTTGCTCCTCCCCAAGCTCAGTCAACCAT
TACTCTGGACATTCAAAATGAAAGTGTTCAGGACGTTTTTAACTACGTTCTGATGGCCTCAGGGCTAAAAGCTAGTCGTC
GGGGTAACACAATTTTCGCTGGGGCTAATCTTTTGCCTTCGGCTCGAAATATTATTACCCGCACTATCCGGTTGAACCAG
GCTAGTGCTGAGTCTGTGGCCAGTACTTTGGCTTCTCAGGGTGCGGAAGTTAACATCCTATTTGAAGGTCAGGAAGATGT
TCAGTTGGCAGAAAATGCGCCGCCAAGGGTAATTAAGCAGCCTCCTACTCTAGTTCCCTTAACTGTTCAAAAACCAGCCA
ACGATTCTTCAGTTTTAATTCTGGAAGGGCTAGTGGTTAGCACTGATCCCCGTTTAAACACTGTAACCCTGGTGGGTGAA
CCCCGTAATGTGGAATTGGCTAGTTCCATGATTACTCAAATGGATGCTCGCCGCCGCCAAGTGGCAGTAAACGTCAAAAT
TATTGACATCAATCTTAATAATATTCAAGATTATGACAGCAGTTTTTCCTTTGGCATAGGAGATAGTTTCTTTGTCCAAG
ACAGTGGTTCTGCGGTCATGCGCTTTGGTGACACGGCTCCAGTTCAGGAAATTGATATCAACAATAACCTGGGGCGAATC
ACTAATCCACCTGCCATTGTTAATCCTTTTCAAGACGGAGAGATTTTCTTTGATCTTAATAGAATAACTAACATTGAAGT
GCCTCTTGGCCCAGGGACCATCCCCATAAATTTCTTCACATCAGGTTCTGGAGCCGTTTCCAACAATCCATTGTTTAATG
GAGTTACTGAATTTCCAATTGTGGAGGTAGATGAACAAGGTCTGTTAACAATCACCCAACCTGAGTTTGGTTTGCCATCT
TTTTATCAATATCCTAAAAAGTTTCAGGCACAAATTGACGCTCAAATACGTAGTGGGAATGCGAAAATTCTCACCGATCC
AACTTTGATTGTTCAAGAAGGCGAGGCTGCTCAAGTTAAGTTAACAGAATCTGTTATTGCCAGCGTTGATACGCAAGTTG
ATACCCAGGGAGATACAGCAGTTAGAACTATTACGCCGGTTCTGGAAGATGTTGGATTAACACTAAATGTCATAGTTGAT
CGAATTGATGACAATGGCTTTATTACTTTGCGCGTTAATCCAATCGTTGCATCTCCTGCTGGAACACAAGTATTTGATAG
TGGTGCTGGCGCCATCAACGAAATAACACTGATAAACAAAAGGGAACTTACTTCCGGTGTAGTTCGGCTGAGGGATGATC
AGACATTCATTCTATCTGGCATTATTTCGGAACTTCAACGTTCTACCACAAGTAAAGTTCCAATTCTAGGGGATTTACCG
GTGATTGGAGCATTGTTTAGGCAGTCCACAGACACAACAGACCGTTCGGAAGTTATTATTCTCATGACCCCCAAGATCAT
TCATGATAGTACAGAGGCGCAGTTTGGCTTCCGTTACAATCCTGACGCGGCTACGGCGGAATTCCTGCGGCAGAAAGGAT
TTCCCGTTCAGGCTCAGCCATAG

Domains

Predicted by InterproScan.

• Bacterial type II and III secretion system protein (Secretin) (576-748)

• Bacterial type II/III secretion system short domain (Secretin_N) (313-420)

References

• [1]: S Yoshihara et al. (2001) Mutational analysis of genes involved in pilus structure, motility and transformation competency in the unicellular motile cyanobacterium Synechocystis sp. PCC 6803. Plant & Cell Physiology 42(1):63-73. [PMID: 11158445]