Detailed information NTG0001412

Experimentally validated

Overview

• Name: pilN
• Type: Machinery gene
• Locus tag: SGL_RS10690
• Genome accession: NC_000911
• Coordinates: 1866843..1867697 (+)
• Length: 284 a.a.
• NCBI ID: WP_010872898.1
• Uniprot ID: -
• Organism: Synechocystis sp. PCC 6803
• Function: type IV pilus biogenesis and function DNA binding and uptake

Function

Competency of the natural transformation was lost in the slr0063, slr1274, slr1275, slr1276, slr1277 and sll1694 mutants and slr0197-disruptant. It was suggested that both DNA uptake for natural transformation and motility are mediated by a specific type IV-like pilus structure, while a putative DNA-binding protein encoded by slr0197 is additionally required for the DNA uptake.

Genomic Context

Location: 1861843..1872697

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SGL_RS10670 (slr1271)	-	1863247..1863987 (+)	741	WP_010872894.1	WecB/TagA/CpsF family glycosyltransferase	-
SGL_RS20030	-	1864239..1865003 (+)	765	WP_010872895.1	iron uptake porin	-
SGL_RS10680 (slr1273)	-	1865033..1865473 (+)	441	WP_010872896.1	hypothetical protein	-
SGL_RS10685 (slr1274)	pilM	1865658..1866761 (+)	1104	WP_010872897.1	type IV pilus biogenesis protein PilM	Machinery gene
SGL_RS10690 (slr1275)	pilN	1866843..1867697 (+)	855	WP_010872898.1	PilN domain-containing protein	Machinery gene
SGL_RS10695 (slr1276)	pilO	1867737..1868564 (+)	828	WP_010872899.1	pilus assembly protein	Machinery gene
SGL_RS10700 (slr1277)	pilQ	1868660..1871002 (+)	2343	WP_020862340.1	type IV pilus secretin PilQ	Machinery gene
SGL_RS10705 (sll1174)	-	1871011..1871835 (-)	825	WP_010872901.1	FkbM family methyltransferase	-
SGL_RS10710 (sll1173)	-	1871855..1872589 (-)	735	WP_010872902.1	FkbM family methyltransferase	-

Sequence

Protein

• Length: 284 a.a.
• Molecular weight: 30034.02 Da
• Isoelectric Point: 4.1639

>NTDB_id=1412 SGL_RS10690 WP_010872898.1 1866843..1867697(+) (pilN) [Synechocystis sp. PCC 6803]
MYSLDVNFLKDRNSDVVRSTQTTTITLTGDDLQKQMPLYIGAGVMVLLPALAGLGVLLINWQKGVAQTNIQQLETELSQL
QGEQQKIQEVEAKTAAAHQEADGLIGVFNQIKPWSALFQEITNQLPQEVQLQSIQQEGNVITLSGFAGNYGSLNDFLLTL
QSSKFLQANKTKLMTANAAALPITGEGATAETGGTEEPSADQAASGVVIPAGVSYTIQTEITNTPDKELITALIANGAAG
LVARFKFLELKGVLDRPAGIPEVAPILEPPAEGAPGAETPPANN

Nucleotide

• Length: 855 bp

>NTDB_id=1412 SGL_RS10690 WP_010872898.1 1866843..1867697(+) (pilN) [Synechocystis sp. PCC 6803]
ATGTATAGCCTAGATGTAAATTTTCTCAAGGATCGTAACTCTGACGTTGTTCGGTCGACCCAAACTACCACTATCACCCT
AACGGGGGACGATCTACAGAAGCAAATGCCGCTTTATATTGGGGCGGGGGTGATGGTACTTTTGCCTGCTTTGGCGGGGT
TGGGAGTACTGCTAATCAATTGGCAAAAGGGTGTTGCTCAAACCAATATCCAACAATTGGAAACGGAGTTATCCCAGCTA
CAGGGGGAACAGCAAAAAATCCAGGAAGTTGAGGCAAAAACGGCGGCGGCTCACCAAGAAGCAGATGGGCTAATTGGCGT
TTTTAATCAAATTAAACCTTGGTCAGCCCTGTTTCAGGAGATAACTAATCAGTTGCCCCAGGAAGTGCAACTCCAAAGTA
TCCAGCAGGAAGGAAATGTAATCACTCTTTCCGGTTTTGCTGGCAACTATGGCTCCCTCAATGATTTTCTGCTGACTTTG
CAAAGCTCGAAGTTTTTGCAGGCTAATAAAACTAAGCTGATGACAGCCAACGCGGCAGCCCTACCTATTACGGGGGAAGG
GGCAACGGCAGAAACCGGGGGCACAGAGGAGCCAAGTGCAGATCAAGCTGCTTCTGGTGTTGTCATTCCAGCGGGAGTGA
GTTATACCATCCAAACGGAAATTACCAATACCCCCGATAAGGAATTGATAACGGCATTGATCGCCAATGGGGCAGCCGGT
TTAGTGGCTCGTTTCAAGTTTCTGGAACTGAAGGGCGTCTTGGATCGACCTGCGGGAATACCGGAGGTTGCGCCTATTTT
GGAGCCACCGGCGGAGGGAGCTCCTGGAGCAGAAACCCCACCAGCTAATAATTGA

Domains

Predicted by InterproScan.

• Fimbrial assembly protein (PilN) (PilN) (116-220)

References

• [1]: S Yoshihara et al. (2001) Mutational analysis of genes involved in pilus structure, motility and transformation competency in the unicellular motile cyanobacterium Synechocystis sp. PCC 6803. Plant & Cell Physiology 42(1):63-73. [PMID: 11158445]