Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   DA70_RS20385 Genome accession   NZ_CP007506
Coordinates   4604976..4605497 (-) Length   173 a.a.
NCBI ID   WP_023594269.1    Uniprot ID   A0A5E4U5T4
Organism   Pandoraea pnomenusa strain RB38     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4599976..4610497
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DA70_RS20365 (DA70_19805) - 4600234..4601784 (-) 1551 WP_025250273.1 hypothetical protein -
  DA70_RS20370 (DA70_19800) - 4601824..4601994 (-) 171 WP_006400971.1 rubredoxin -
  DA70_RS20375 (DA70_19795) thiD 4602205..4603044 (+) 840 WP_023872296.1 bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase -
  DA70_RS20380 (DA70_19790) - 4603122..4604891 (-) 1770 WP_144347216.1 O-antigen ligase family protein -
  DA70_RS20385 (DA70_19785) pilA 4604976..4605497 (-) 522 WP_023594269.1 pilin Machinery gene
  DA70_RS20390 (DA70_19780) - 4605735..4606463 (-) 729 WP_023594268.1 TerC family protein -
  DA70_RS20395 (DA70_19775) sucD 4606666..4607547 (-) 882 WP_023594267.1 succinate--CoA ligase subunit alpha -
  DA70_RS20400 (DA70_19770) sucC 4607629..4608795 (-) 1167 WP_023594266.1 ADP-forming succinate--CoA ligase subunit beta -
  DA70_RS20405 (DA70_19765) - 4608792..4609478 (-) 687 WP_023594265.1 DUF2889 domain-containing protein -
  DA70_RS20410 (DA70_19760) recX 4609798..4610340 (-) 543 WP_023872300.1 recombination regulator RecX -

Sequence

Protein


Download         Length: 173 a.a.        Molecular weight: 17654.27 Da        Isoelectric Point: 9.8858

>NTDB_id=120100 DA70_RS20385 WP_023594269.1 4604976..4605497(-) (pilA) [Pandoraea pnomenusa strain RB38]
MRRFGHRTTGLAKSRGFTLIELMIVLAIIGVLVTAGVPYLQNYLVRARVVEGLGAAASAKALVSENAMHGAPFNSGWQAP
SATDNVNAVTIDSVSGNVTVAYTQRAGDGALVLVPTSAGPDGKPMALTVGKAPEGQIVWTCYAQGREGAPGGATLPAKLA
PPECRGAATQRGA

Nucleotide


Download         Length: 522 bp        

>NTDB_id=120100 DA70_RS20385 WP_023594269.1 4604976..4605497(-) (pilA) [Pandoraea pnomenusa strain RB38]
ATGCGTCGTTTCGGCCACCGGACCACCGGTCTCGCCAAATCCCGGGGATTCACCCTGATCGAGCTGATGATCGTGCTGGC
GATCATCGGCGTGTTGGTCACGGCAGGGGTTCCGTATCTGCAAAACTATCTGGTGCGTGCGCGTGTCGTCGAGGGACTGG
GCGCGGCCGCGTCGGCCAAGGCGCTCGTGAGCGAGAACGCCATGCACGGCGCCCCGTTCAATAGCGGTTGGCAAGCGCCG
AGTGCGACCGACAACGTGAACGCCGTGACCATCGACTCGGTGAGCGGCAACGTCACGGTCGCCTACACCCAGCGCGCTGG
CGACGGTGCTCTGGTGCTGGTGCCCACGAGCGCCGGTCCCGACGGCAAGCCCATGGCGCTGACGGTCGGCAAGGCGCCTG
AAGGCCAGATCGTATGGACGTGTTACGCACAAGGTCGCGAGGGCGCACCCGGCGGTGCCACGCTGCCCGCGAAGCTCGCG
CCTCCCGAGTGCCGTGGCGCGGCGACCCAGCGGGGCGCCTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(45-165)

Prokaryotic N-terminal methylation motif (N_methyl)

(13-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5E4U5T4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

48

100

0.486
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

34.375

100

0.382
  pilA2 Legionella pneumophila str. Paris

39.873

91.329

0.364

Multiple sequence alignment