Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   NTHI477_RS06645 Genome accession   NZ_CP007470
Coordinates   1391017..1391709 (-) Length   230 a.a.
NCBI ID   WP_044332408.1    Uniprot ID   -
Organism   Haemophilus influenzae strain 477     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1386017..1396709
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NTHI477_RS06610 (NTHI477_01335) - 1387601..1387807 (-) 207 WP_005666693.1 heavy-metal-associated domain-containing protein -
  NTHI477_RS06615 (NTHI477_01336) - 1387881..1388087 (-) 207 WP_005666693.1 heavy-metal-associated domain-containing protein -
  NTHI477_RS06620 (NTHI477_01337) - 1388161..1388367 (-) 207 WP_005666693.1 heavy-metal-associated domain-containing protein -
  NTHI477_RS06625 (NTHI477_01338) - 1388453..1388659 (-) 207 WP_005666692.1 heavy-metal-associated domain-containing protein -
  NTHI477_RS06630 (NTHI477_01339) cueR 1388736..1389122 (+) 387 WP_005648963.1 Cu(I)-responsive transcriptional regulator -
  NTHI477_RS06635 (NTHI477_01340) metJ 1389136..1389453 (-) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  NTHI477_RS06640 (NTHI477_01341) rho 1389701..1390963 (+) 1263 WP_005648966.1 transcription termination factor Rho -
  NTHI477_RS06645 (NTHI477_01342) pilD 1391017..1391709 (-) 693 WP_044332408.1 prepilin peptidase Machinery gene
  NTHI477_RS06650 (NTHI477_01343) pilC 1391706..1392923 (-) 1218 WP_014550481.1 type II secretion system F family protein Machinery gene
  NTHI477_RS06655 (NTHI477_01344) pilB 1392920..1394314 (-) 1395 WP_011271972.1 GspE/PulE family protein Machinery gene
  NTHI477_RS06660 (NTHI477_01345) pilA 1394311..1394760 (-) 450 WP_011271973.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NTHI477_RS06665 (NTHI477_01346) ampD 1394875..1395435 (+) 561 WP_011271974.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 230 a.a.        Molecular weight: 26702.71 Da        Isoelectric Point: 8.8532

>NTDB_id=119699 NTHI477_RS06645 WP_044332408.1 1391017..1391709(-) (pilD) [Haemophilus influenzae strain 477]
MIYFTMFLLGGILGIALWFYLSGFITRLQQEIYATYIELFPQNFLSFQPSVSSIQQKKCGHILRYFLNVGVEFIFLQIAF
KDSTFAIWLGLTLLILWAISYLDWHYQLISTTLCLWLLTIGLFGADNNFSLLTLSESIKSAASFFIVFYAIYWIAKFYYG
KEAFGRGDYWLAMALGSFIHLETLPHFLLLASVLGICFSLIRKKKKEFIPFAPFMNLSAVIIYFVKYYGY

Nucleotide


Download         Length: 693 bp        

>NTDB_id=119699 NTHI477_RS06645 WP_044332408.1 1391017..1391709(-) (pilD) [Haemophilus influenzae strain 477]
ATGATTTACTTCACAATGTTTTTATTAGGCGGTATCTTAGGGATCGCATTGTGGTTCTACCTATCTGGTTTTATTACCCG
TTTACAGCAAGAGATTTATGCGACTTATATTGAATTATTTCCACAAAACTTTCTGTCATTTCAGCCTAGCGTTTCCTCTA
TTCAACAAAAAAAGTGCGGTCATATTTTGAGGTATTTTTTAAATGTTGGGGTAGAATTTATATTTTTACAAATTGCCTTC
AAGGACTCTACTTTCGCTATATGGCTAGGGCTCACACTTCTTATCCTTTGGGCAATCAGTTATCTTGATTGGCACTATCA
ACTTATTTCTACTACACTCTGTTTATGGCTACTTACAATCGGTTTATTTGGAGCAGACAATAATTTTTCATTGCTTACGT
TATCTGAAAGCATAAAAAGTGCGGCAAGTTTTTTTATTGTTTTCTACGCAATCTATTGGATTGCAAAATTCTATTACGGA
AAAGAAGCCTTTGGACGGGGAGATTATTGGCTAGCAATGGCATTAGGAAGTTTTATTCATTTAGAAACCTTACCGCACTT
TTTATTATTAGCCTCAGTTCTTGGAATATGTTTTTCGCTTATTCGTAAAAAGAAAAAAGAATTTATACCTTTTGCCCCTT
TTATGAACTTATCAGCTGTCATCATTTATTTCGTCAAATATTACGGATATTAA

Domains

Predicted by InterproScan.

Type IV leader peptidase family (Peptidase_A24)

(92-199)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Haemophilus influenzae 86-028NP

89.13

100

0.891
  pilD Haemophilus influenzae Rd KW20

88.696

100

0.887

Multiple sequence alignment