Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   CD58_RS24405 Genome accession   NZ_CP007410
Coordinates   5693481..5695070 (+) Length   529 a.a.
NCBI ID   WP_025215540.1    Uniprot ID   -
Organism   Pseudomonas brassicacearum strain DF41     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5688481..5700070
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CD58_RS24390 (CD58_25350) pgeF 5690237..5690962 (-) 726 WP_025215536.1 peptidoglycan editing factor PgeF -
  CD58_RS24395 (CD58_25355) rluD 5690959..5691921 (-) 963 WP_025215537.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  CD58_RS24400 (CD58_25365) - 5692073..5693089 (+) 1017 WP_025215538.1 outer membrane protein assembly factor BamD -
  CD58_RS29575 (CD58_25370) - 5693258..5693491 (+) 234 WP_025215539.1 PP0621 family protein -
  CD58_RS24405 (CD58_25375) pilS 5693481..5695070 (+) 1590 WP_025215540.1 two-component system sensor histidine kinase NtrB Regulator
  CD58_RS24410 (CD58_25380) pilR 5695067..5696413 (+) 1347 WP_025215541.1 sigma-54-dependent transcriptional regulator Regulator
  CD58_RS24415 (CD58_25385) - 5696600..5696965 (-) 366 WP_025215542.1 DUF6124 family protein -
  CD58_RS31145 (CD58_25395) - 5697987..5698151 (-) 165 WP_158482165.1 hypothetical protein -
  CD58_RS24425 (CD58_25400) thiO 5698297..5699397 (-) 1101 WP_025215544.1 glycine oxidase ThiO -
  CD58_RS24430 (CD58_25405) - 5699452..5699853 (-) 402 WP_025215545.1 type IV pilin protein -

Sequence

Protein


Download         Length: 529 a.a.        Molecular weight: 58718.41 Da        Isoelectric Point: 7.0772

>NTDB_id=119274 CD58_RS24405 WP_025215540.1 5693481..5695070(+) (pilS) [Pseudomonas brassicacearum strain DF41]
MSAEASSPRVKQAQRLLRLYHLYRLSIGITLVLLISSNMDNRLLEFANDDLLRSGSWLYLVLNILLVVFLENTRRPARLF
GLALTDVLLLSWLFFAAGGAPSAIGNLLIVSVAIGNTLLRGRIGLLIAAVATIGIVSSTFFLGLSDSNRPSSYLQAGTLG
ALCFAAALLVQGLTRRLEASETLAEQRASEVIGLEALNALILQRMRTGILVLDRERRVQLANESALNLLGMHDLVGQPID
DYSMALVERLQLWLNNPSLRPQSLTISGTGLTLQPSFIALGHNDQHQTLVFLEDLAQVAQHAQQLKLASLGRLTAGIAHE
IRNPLGAISHAAQLLRESEELNDADRRLTQIIQDHSQRMNRVIENVLQLSRRQQTTPQRLDLRAWLAQFVRQAHESAAEH
QQLHLSIDPGDYTTLMDPDQLTQVLDNLLRNAWRHSAMLHEQAEAWLKLFIDPHSQLSTLDILDNGPGVTAEQQAHLFEP
FFTTSNQGTGLGLYLSRELCESNQARLDFKPRQGGGCFRITFAHGRKQI

Nucleotide


Download         Length: 1590 bp        

>NTDB_id=119274 CD58_RS24405 WP_025215540.1 5693481..5695070(+) (pilS) [Pseudomonas brassicacearum strain DF41]
GTGAGCGCTGAGGCCTCAAGCCCGCGCGTCAAACAGGCGCAGCGCCTGCTGCGTCTCTATCACCTGTACCGCCTGAGCAT
CGGCATCACCCTGGTGCTGCTGATCTCCAGCAACATGGACAATCGCCTGCTGGAGTTCGCCAACGACGACCTGCTGCGCA
GCGGCAGCTGGTTGTACCTGGTGCTGAACATCCTGCTGGTGGTGTTCCTTGAAAACACCCGCCGCCCTGCCCGGCTGTTC
GGCCTCGCCCTCACCGACGTGCTGTTGCTGTCGTGGCTGTTCTTCGCCGCTGGCGGCGCCCCCAGTGCCATCGGCAACCT
GCTCATCGTCTCGGTCGCCATCGGCAACACGCTGTTGCGCGGTCGAATCGGCCTGTTGATCGCCGCCGTCGCCACGATTG
GTATCGTCAGCTCGACGTTCTTCCTCGGCCTGAGCGATTCGAACCGCCCCAGCAGTTACTTGCAGGCCGGCACCCTGGGG
GCGTTGTGTTTTGCTGCCGCCCTGCTGGTACAAGGCCTGACCCGGCGACTGGAAGCCAGTGAAACCCTGGCCGAGCAACG
CGCCAGCGAAGTGATCGGCCTCGAAGCGCTCAACGCGCTGATCCTGCAACGCATGCGCACCGGCATCCTGGTGCTGGATC
GCGAGCGCCGGGTGCAACTGGCCAACGAAAGCGCGCTGAACCTGCTGGGCATGCACGATCTGGTCGGCCAGCCGATCGAC
GACTATTCCATGGCCCTGGTGGAGCGCCTGCAACTCTGGCTGAACAACCCCAGCCTACGCCCGCAGAGCCTGACCATCAG
CGGCACCGGCCTCACCCTGCAACCGAGCTTCATCGCCCTGGGACACAACGACCAGCATCAGACCCTGGTATTCCTCGAAG
ACCTGGCCCAGGTCGCCCAGCACGCCCAGCAACTCAAACTCGCCTCCCTCGGGCGCCTGACCGCCGGTATCGCCCATGAA
ATCCGCAACCCTTTGGGGGCGATCAGTCATGCGGCGCAATTGCTGCGCGAATCCGAGGAACTGAACGACGCGGATCGACG
TCTGACGCAGATTATTCAAGACCACTCCCAACGAATGAATCGCGTCATTGAAAACGTCCTGCAACTGTCCCGCCGCCAGC
AAACCACGCCCCAACGCCTGGATCTGCGGGCCTGGCTCGCCCAGTTCGTCCGCCAAGCCCACGAAAGCGCAGCCGAGCAC
CAACAATTGCACCTGAGCATCGACCCGGGCGACTACACCACGCTGATGGATCCCGACCAGTTGACCCAGGTGCTCGACAA
CCTGTTGCGCAACGCCTGGCGCCACAGCGCCATGCTCCATGAACAGGCCGAGGCCTGGCTGAAACTGTTCATCGACCCCC
ATAGCCAGCTATCCACACTGGATATCCTCGACAACGGCCCCGGCGTCACAGCGGAGCAGCAAGCGCATCTGTTCGAGCCC
TTCTTCACCACCAGCAACCAGGGCACCGGCCTTGGGCTCTATCTGTCCCGTGAGCTGTGCGAAAGCAACCAGGCCCGCCT
AGACTTCAAACCACGCCAAGGCGGCGGCTGCTTTCGCATCACCTTTGCTCACGGACGGAAACAGATTTGA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(309-374)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-522)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

59.091

99.811

0.59

Multiple sequence alignment