Detailed information NTG0119229

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: Q433_RS12960
• Genome accession: NZ_CP007409
• Coordinates: 2493362..2493979 (-)
• Length: 205 a.a.
• NCBI ID: WP_004398514.1
• Uniprot ID: A0AAE2SLR2
• Organism: Bacillus subtilis subsp. subtilis str. OH 131.1 strain OH131.1
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2488362..2498979

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Q433_RS12935 (Q433_13970)	rpsT	2488664..2488930 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
Q433_RS12940 (Q433_13975)	holA	2488945..2489988 (-)	1044	WP_003229987.1	DNA polymerase III subunit delta	-
Q433_RS21595	-	2490028..2490177 (-)	150	WP_003229985.1	hypothetical protein	-
Q433_RS12945 (Q433_13985)	yqzM	2490218..2490352 (+)	135	WP_003229983.1	YqzM family protein	-
Q433_RS12950 (Q433_13990)	comEC	2490392..2492722 (-)	2331	WP_009967776.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
Q433_RS12955 (Q433_13995)	comEB	2492726..2493295 (-)	570	WP_003229978.1	ComE operon protein 2	-
Q433_RS12960 (Q433_14000)	comEA	2493362..2493979 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
Q433_RS12965 (Q433_14005)	comER	2494063..2494884 (+)	822	WP_004398597.1	late competence protein ComER	-
Q433_RS12970 (Q433_14010)	yqeM	2494950..2495693 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
Q433_RS12975 (Q433_14015)	rsfS	2495690..2496046 (-)	357	WP_003229971.1	ribosome silencing factor	-
Q433_RS12980 (Q433_14020)	yqeK	2496064..2496624 (-)	561	WP_004399059.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
Q433_RS12985 (Q433_14025)	nadD	2496614..2497183 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
Q433_RS12990 (Q433_14030)	yhbY	2497195..2497485 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
Q433_RS12995 (Q433_14035)	aroE	2497479..2498321 (-)	843	WP_003229967.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21769.45 Da
• Isoelectric Point: 4.7220

>NTDB_id=119229 Q433_RS12960 WP_004398514.1 2493362..2493979(-) (comEA) [Bacillus subtilis subsp. subtilis str. OH 131.1 strain OH131.1]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=119229 Q433_RS12960 WP_004398514.1 2493362..2493979(-) (comEA) [Bacillus subtilis subsp. subtilis str. OH 131.1 strain OH131.1]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39