Detailed information NTG0118241

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: AJ82_RS12050
• Genome accession: NZ_CP007244
• Coordinates: 2547597..2548211 (-)
• Length: 204 a.a.
• NCBI ID: WP_025285016.1
• Uniprot ID: -
• Organism: Bacillus velezensis TrigoCor1448
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2542597..2553211

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AJ82_RS12025 (AJ82_13470)	rpsT	2542900..2543166 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
AJ82_RS12030 (AJ82_13475)	holA	2543183..2544224 (-)	1042	Protein_2392	DNA polymerase III subunit delta	-
AJ82_RS20030	-	2544264..2544415 (-)	152	Protein_2393	hypothetical protein	-
AJ82_RS19495 (AJ82_13485)	-	2544456..2544590 (+)	135	WP_003152870.1	YqzM family protein	-
AJ82_RS12040 (AJ82_13490)	comEC	2544609..2546960 (-)	2352	WP_025285015.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
AJ82_RS12045 (AJ82_13495)	-	2546961..2547530 (-)	570	WP_003152868.1	ComE operon protein 2	-
AJ82_RS12050 (AJ82_13500)	comEA	2547597..2548211 (-)	615	WP_025285016.1	helix-hairpin-helix domain-containing protein	Machinery gene
AJ82_RS12055 (AJ82_13505)	comER	2548270..2549091 (+)	822	WP_015240246.1	late competence protein ComER	-
AJ82_RS12060 (AJ82_13510)	-	2549160..2549897 (-)	738	WP_015240247.1	class I SAM-dependent methyltransferase	-
AJ82_RS12065 (AJ82_13515)	rsfS	2549894..2550250 (-)	357	WP_007408260.1	ribosome silencing factor	-
AJ82_RS12070 (AJ82_13520)	yqeK	2550268..2550828 (-)	561	WP_025285017.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
AJ82_RS12075 (AJ82_13525)	-	2550818..2551387 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
AJ82_RS12080 (AJ82_13530)	yhbY	2551398..2551688 (-)	291	WP_025285018.1	ribosome assembly RNA-binding protein YhbY	-
AJ82_RS12085 (AJ82_13535)	aroE	2551682..2552524 (-)	843	WP_025285019.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22237.37 Da
• Isoelectric Point: 5.5211

>NTDB_id=118241 AJ82_RS12050 WP_025285016.1 2547597..2548211(-) (comEA) [Bacillus velezensis TrigoCor1448]
MTKAMMTERLRRNLKILGACLVAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=118241 AJ82_RS12050 WP_025285016.1 2547597..2548211(-) (comEA) [Bacillus velezensis TrigoCor1448]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGTAGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGACAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGCACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.639	93.627	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA	Latilactobacillus sakei subsp. sakei 23K	31.897	100	0.363
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363